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DEDD2 Antibody (BS-7063R) in IHC (P)
Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with DEDD2 Polyclonal Antibody, Unconjugated (bs-7063R) at 1:400 overnight at 4°C, DAB staining.
Product Details
BS-7063R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human DEDD2, amino acids 201-300.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Apoptotic signals are often triggered by cell surface death receptors through protein-protein interactions mediated by conserved domains such as the death effector domain. A novel death effector domain (DED)-containing protein, DEDD2, has been recently identified. Over-expression of DEDD2 in transfected cells induces moderate apoptosis and results in substantial sensitization to apoptosis induced by Fas, TRAIL, and FADD. More recently, it has been shown that DEDD2 can bind caspase-8 and -10 in addition to FLIP but not FADD. Like the related protein DEDD, DEDD2 translocates from the cytosol to the nucleus upon induction of apoptosis, and it has been suggested that DEDD2 may target caspase-8 to the nucleus and that DEDD2 thus plays a critical role in death receptor-induced apoptosis. At least two alternatively spliced transcript variants encoding distinct isoforms have been found for DEDD2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: death effector domain containing 2; death effector domain-containing DNA-binding protein 2; DED-containing protein FLAME-3; DNA-binding death effector domain-containing protein 2; FADD-like anti-apoptotic molecule 3
Gene Aliases: 2410050E11Rik; DEDD2; FLAME-3; FLAME3; PSEC0004
UniProt ID: (Human) Q8WXF8, (Mouse) Q8QZV0
Entrez Gene ID: (Human) 162989, (Mouse) 67379, (Rat) 687118
transcription, DNA-templated
RNA processing
extrinsic apoptotic signaling pathway via death domain receptors
rRNA catabolic process
cellular homeostasis
apoptotic nuclear changes
intracellular signal transduction
negative regulation of transcription, DNA-templated
positive regulation of extrinsic apoptotic signaling pathway
regulation of transcription, DNA-templated
apoptotic process
regulation of apoptotic process
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