Proteintech

DEK Polyclonal Antibody

View all (22) DEK antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (13)

DEK Antibody in Immunocytochemistry (ICC/IF)

DEK Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 13

DEK Antibody (16448-1-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DEK antibody (Product # 16448-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

DEK Antibody in Immunocytochemistry (ICC/IF)

DEK Antibody in Immunocytochemistry (ICC/IF)

DEK Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DEK Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DEK Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DEK Antibody in Western Blot (WB)

DEK Antibody in Western Blot (WB)

DEK Antibody in Western Blot (WB)

DEK Antibody in Western Blot (WB)

DEK Antibody in Western Blot (WB)

DEK Antibody in Western Blot (WB)

DEK Antibody in Western Blot (WB)

DEK Antibody in Immunoprecipitation (IP)

Product Image Gallery

DEK Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DEK antibody (Product # 16448-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

DEK Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Hela cells, using DEK antibody 16448-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

DEK Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded Mouse brain tissue slide using (Product # 16448-1-AP) DEK antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DEK Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human brain using 16448-1-AP (DEK antibody) at dilution of 1:100 (under 40x lens).

DEK Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human brain using 16448-1-AP (DEK antibody) at dilution of 1:100 (under 10x lens).

DEK Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with (Product # 16448-1-AP) DEK antibody at dilution of 1:1,500 incubated at room temperature for 1.5 hours.

DEK Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 16448-1-AP (DEK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

DEK Antibody in Western Blot (WB) — Rat brain tissue were subjected to SDS PAGE followed by western blot with 16448-1-AP (DEK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

DEK Antibody in Western Blot (WB) — K-562 cells were subjected to SDS PAGE followed by western blot with 16448-1-AP (DEK antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

DEK Antibody in Western Blot (WB) — A431 cells were subjected to SDS PAGE followed by western blot with 16448-1-AP (DEK antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

DEK Antibody in Western Blot (WB) — A375 cells were subjected to SDS PAGE followed by western blot with 16448-1-AP (DEK antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

DEK Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 16448-1-AP (DEK antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

DEK Antibody in Immunoprecipitation (IP) — IP result of anti-DEK (IP:16448-1-AP, 4ug; Detection:16448-1-AP 1:500) with HeLa cells lysate 1200ug.

Product Details

16448-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 15 publications 15 publications

Immunohistochemistry (IHC)

-

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

-

Immunocytochemistry (ICC/IF)

1:10-1:100

View 5 publications 5 publications

Immunoprecipitation (IP)

0.5-4.0 µg

-

ChIP assay (ChIP)

-

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

-

View 2 publications 2 publications

Not applicable (N/A)

-

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

DEK Fusion Protein Ag8225 (1-375 aa encoded by BC035259)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.29 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MSASAPAAE GEGTPTQPAS EKEPEMPGPR EESEEEEDED DEEEEEEEKE KSLIVEGKRE KKKVERLTMQ VSSLQREPFT IAQGKGQKLC EIERIHFFLS KKKTDELRNL HKLLYNRPGT VSSLKKNVGQ FSGFPFEKGS VQYKKKEEML KKFRNAMLKS ICEVLDLERS GVNSELVKRI LNFLMHPKPS GKPLPKSKKT CSKGSKKERN SSGMARKAKR TKCPEILSDE SSSDEDEKKN KEESSDDEDK ESEEEPPKKT AKREKPKQKA TSKSKKSVKS ANVKKADSST TKKNQNSSKK ESESEDSSDD EPLIKKLKKP PTDEELKETI KKLLASANLE EVTMKQICKK VYENYPTYDL TERKDFIKTT VKELIS (1-375 aa encoded by B C035259)

Target Information

DEK is a protein with one SAP domain. This protein binds to cruciform and superhelical DNA and induces positive supercoils into closed circular DNA, and is also involved in splice site selection during mRNA processing. Chromosomal aberrations involving this region, increased expression of this gene, and the presence of antibodies against this protein are all associated with various diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Exosomal DEK removes chemoradiotherapy resistance by triggering quiescence exit of breast cancer stem cells.

Oncogene

Yang YS,Jia XZ,Lu QY,Cai SL,Huang XT,Yang SH,Wood C,Wang YH,Zhou JJ,Chen YD,Yang JS,Yang WJ

Fri Apr 01 00:00:00 UTC 2022

Exosomal DEK removes chemoradiotherapy resistance by triggering quiescence exit of breast cancer stem cells.

Oncogene

Yang YS,Jia XZ,Lu QY,Cai SL,Huang XT,Yang SH,Wood C,Wang YH,Zhou JJ,Chen YD,Yang JS,Yang WJ

Fri Apr 01 00:00:00 UTC 2022

Exosomal DEK removes chemoradiotherapy resistance by triggering quiescence exit of breast cancer stem cells.

Oncogene

Yang YS,Jia XZ,Lu QY,Cai SL,Huang XT,Yang SH,Wood C,Wang YH,Zhou JJ,Chen YD,Yang JS,Yang WJ

Fri Apr 01 00:00:00 UTC 2022

Exosomal DEK removes chemoradiotherapy resistance by triggering quiescence exit of breast cancer stem cells.

Oncogene

Yang YS,Jia XZ,Lu QY,Cai SL,Huang XT,Yang SH,Wood C,Wang YH,Zhou JJ,Chen YD,Yang JS,Yang WJ

Fri Apr 01 00:00:00 UTC 2022

Exosomal DEK removes chemoradiotherapy resistance by triggering quiescence exit of breast cancer stem cells.

Oncogene

Yang YS,Jia XZ,Lu QY,Cai SL,Huang XT,Yang SH,Wood C,Wang YH,Zhou JJ,Chen YD,Yang JS,Yang WJ

Fri Apr 01 00:00:00 UTC 2022

Exosomal DEK removes chemoradiotherapy resistance by triggering quiescence exit of breast cancer stem cells.

Oncogene

Yang YS,Jia XZ,Lu QY,Cai SL,Huang XT,Yang SH,Wood C,Wang YH,Zhou JJ,Chen YD,Yang JS,Yang WJ

Fri Apr 01 00:00:00 UTC 2022

ATRX limits the accessibility of histone H3-occupied HSV genomes during lytic infection.

PLoS pathogens

Cabral JM,Cushman CH,Sodroski CN,Knipe DM

Thu Apr 01 00:00:00 UTC 2021

Nuclear DEK preserves hematopoietic stem cells potential via NCoR1/HDAC3-Akt1/2-mTOR axis.

The Journal of experimental medicine

Chen Z,Huo D,Li L,Liu Z,Li Z,Xu S,Huang Y,Wu W,Zhou C,Liu Y,Kuang M,Wu F,Li H,Qian P,Song G,Wu X,Chen J,Hou Y

Mon May 03 00:00:00 UTC 2021

DEK is highly expressed in breast cancer and is associated with malignant phenotype and progression.

Oncology letters

Yang MQ,Bai LL,Wang Z,Lei L,Zheng YW,Li ZH,Huang WJ,Liu CC,Xu HT

Tue Jun 01 00:00:00 UTC 2021

DEK is highly expressed in breast cancer and is associated with malignant phenotype and progression.

Oncology letters

Yang MQ,Bai LL,Wang Z,Lei L,Zheng YW,Li ZH,Huang WJ,Liu CC,Xu HT

Tue Jun 01 00:00:00 UTC 2021

DEK Expression in Breast Cancer Cells Leads to the Alternative Activation of Tumor Associated Macrophages.

Cancers

Pease NA,Shephard MS,Sertorio M,Waltz SE,Vinnedge LMP

Fri Jul 17 00:00:00 UTC 2020

Protocol for Isolation and Characterization of In Situ Fixed Quiescent Muscle Stem Cells.

STAR protocols

Yue L,Cheung TH

Fri Dec 18 00:00:00 UTC 2020

Loss of DEK Expression Induces Alzheimer's Disease Phenotypes in Differentiated SH-SY5Y Cells.

Frontiers in molecular neuroscience

Greene AN,Parks LG,Solomon MB,Privette Vinnedge LM

Fri Mar 11 00:00:00 UTC 2022

DEK promotes the proliferation and invasion of lung cancers and indicates poor prognosis in lung adenocarcinomas.

Oncology reports

Yang MQ,Bai LL,Lei L,Zheng YW,Wang Z,Li ZH,Liu CC,Huang WJ,Xu HT

Wed Apr 01 00:00:00 UTC 2020

Dek Modulates Global Intron Retention during Muscle Stem Cells Quiescence Exit.

Developmental cell

Yue L,Wan R,Luan S,Zeng W,Cheung TH

Mon Jun 22 00:00:00 UTC 2020

Overexpression of miR-489 derails mammary hierarchy structure and inhibits HER2/neu-induced tumorigenesis.

Oncogene

Patel Y,Soni M,Awgulewitsch A,Kern MJ,Liu S,Shah N,Singh UP,Chen H

Tue Jan 01 00:00:00 UTC 2019

Overexpression of miR-489 derails mammary hierarchy structure and inhibits HER2/neu-induced tumorigenesis.

Oncogene

Patel Y,Soni M,Awgulewitsch A,Kern MJ,Liu S,Shah N,Singh UP,Chen H

Tue Jan 01 00:00:00 UTC 2019

DEK Is a Potential Biomarker Associated with Malignant Phenotype in Gastric Cancer Tissues and Plasma.

International journal of molecular sciences

Lee KF,Tsai MM,Tsai CY,Huang CG,Ou YH,Hsieh CC,Hsieh HL,Wang CS,Lin KH

Wed Nov 13 00:00:00 UTC 2019

Argininosuccinate synthetase 1 contributes to gastric cancer invasion and progression by modulating autophagy.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Tsai CY,Chi HC,Chi LM,Yang HY,Tsai MM,Lee KF,Huang HW,Chou LF,Cheng AJ,Yang CW,Wang CS,Lin KH

Tue May 01 00:00:00 UTC 2018

Argininosuccinate synthetase 1 contributes to gastric cancer invasion and progression by modulating autophagy.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Tsai CY,Chi HC,Chi LM,Yang HY,Tsai MM,Lee KF,Huang HW,Chou LF,Cheng AJ,Yang CW,Wang CS,Lin KH

Tue May 01 00:00:00 UTC 2018

Neuroanatomical Distribution of DEK Protein in Corticolimbic Circuits Associated with Learning and Memory in Adult Male and Female Mice.

Neuroscience

Ghisays V,Nguyen ET,Streicher J,Pease NA,Fitzgerald M,Estrada CM,Franco-Villanueva A,Privette Vinnedge L,Solomon MB

Sat Feb 10 00:00:00 UTC 2018

Neuroanatomical Distribution of DEK Protein in Corticolimbic Circuits Associated with Learning and Memory in Adult Male and Female Mice.

Neuroscience

Ghisays V,Nguyen ET,Streicher J,Pease NA,Fitzgerald M,Estrada CM,Franco-Villanueva A,Privette Vinnedge L,Solomon MB

Sat Feb 10 00:00:00 UTC 2018

Neuroanatomical Distribution of DEK Protein in Corticolimbic Circuits Associated with Learning and Memory in Adult Male and Female Mice.

Neuroscience

Ghisays V,Nguyen ET,Streicher J,Pease NA,Fitzgerald M,Estrada CM,Franco-Villanueva A,Privette Vinnedge L,Solomon MB

Sat Feb 10 00:00:00 UTC 2018

Oncogene DEK is highly expressed in lung cancerous tissues and positively regulates cell proliferation as well as invasion.

Oncology letters

Zhou QC,Deng XF,Yang J,Jiang H,Qiao MX,Liu HH,Qian Z,Hou LL,Hu HG

Fri Jun 01 00:00:00 UTC 2018

Oncogene DEK is highly expressed in lung cancerous tissues and positively regulates cell proliferation as well as invasion.

Oncology letters

Zhou QC,Deng XF,Yang J,Jiang H,Qiao MX,Liu HH,Qian Z,Hou LL,Hu HG

Fri Jun 01 00:00:00 UTC 2018

Oncogene DEK is highly expressed in lung cancerous tissues and positively regulates cell proliferation as well as invasion.

Oncology letters

Zhou QC,Deng XF,Yang J,Jiang H,Qiao MX,Liu HH,Qian Z,Hou LL,Hu HG

Fri Jun 01 00:00:00 UTC 2018

Oncogene DEK is highly expressed in lung cancerous tissues and positively regulates cell proliferation as well as invasion.

Oncology letters

Zhou QC,Deng XF,Yang J,Jiang H,Qiao MX,Liu HH,Qian Z,Hou LL,Hu HG

Fri Jun 01 00:00:00 UTC 2018

SPOP promotes ATF2 ubiquitination and degradation to suppress prostate cancer progression.

Journal of experimental & clinical cancer research : CR

Ma J,Chang K,Peng J,Shi Q,Gan H,Gao K,Feng K,Xu F,Zhang H,Dai B,Zhu Y,Shi G,Shen Y,Zhu Y,Qin X,Li Y,Zhang P,Ye D,Wang C

Wed Jul 11 00:00:00 UTC 2018

SPOP promotes ATF2 ubiquitination and degradation to suppress prostate cancer progression.

Journal of experimental & clinical cancer research : CR

Ma J,Chang K,Peng J,Shi Q,Gan H,Gao K,Feng K,Xu F,Zhang H,Dai B,Zhu Y,Shi G,Shen Y,Zhu Y,Qin X,Li Y,Zhang P,Ye D,Wang C

Wed Jul 11 00:00:00 UTC 2018

Dek overexpression in murine epithelia increases overt esophageal squamous cell carcinoma incidence.

PLoS genetics

Matrka MC,Cimperman KA,Haas SR,Guasch G,Ehrman LA,Waclaw RR,Komurov K,Lane A,Wikenheiser-Brokamp KA,Wells SI

Thu Mar 01 00:00:00 UTC 2018

Dek overexpression in murine epithelia increases overt esophageal squamous cell carcinoma incidence.

PLoS genetics

Matrka MC,Cimperman KA,Haas SR,Guasch G,Ehrman LA,Waclaw RR,Komurov K,Lane A,Wikenheiser-Brokamp KA,Wells SI

Thu Mar 01 00:00:00 UTC 2018

DEK is required for homologous recombination repair of DNA breaks.

Scientific reports

Smith EA,Gole B,Willis NA,Soria R,Starnes LM,Krumpelbeck EF,Jegga AG,Ali AM,Guo H,Meetei AR,Andreassen PR,Kappes F,Vinnedge LM,Daniel JA,Scully R,Wiesmüller L,Wells SI

Mon Mar 20 00:00:00 UTC 2017

DEK is required for homologous recombination repair of DNA breaks.

Scientific reports

Smith EA,Gole B,Willis NA,Soria R,Starnes LM,Krumpelbeck EF,Jegga AG,Ali AM,Guo H,Meetei AR,Andreassen PR,Kappes F,Vinnedge LM,Daniel JA,Scully R,Wiesmüller L,Wells SI

Mon Mar 20 00:00:00 UTC 2017

Fbw7 regulates apoptosis in activated B-cell like diffuse large B-cell lymphoma by targeting Stat3 for ubiquitylation and degradation.

Journal of experimental & clinical cancer research : CR

Yao S,Xu F,Chen Y,Ge Y,Zhang F,Huang H,Li L,Lin D,Luo X,Xu J,Luo D,Zhu X,Liu Y

Tue Jan 10 00:00:00 UTC 2017

Fbw7 regulates apoptosis in activated B-cell like diffuse large B-cell lymphoma by targeting Stat3 for ubiquitylation and degradation.

Journal of experimental & clinical cancer research : CR

Yao S,Xu F,Chen Y,Ge Y,Zhang F,Huang H,Li L,Lin D,Luo X,Xu J,Luo D,Zhu X,Liu Y

Tue Jan 10 00:00:00 UTC 2017

Regulation of DEK expression by AP-2α and methylation level of DEK promoter in hepatocellular carcinoma.

Oncology reports

Qiao MX,Li C,Zhang AQ,Hou LL,Yang J,Hu HG

Sat Oct 01 00:00:00 UTC 2016

Regulation of DEK expression by AP-2α and methylation level of DEK promoter in hepatocellular carcinoma.

Oncology reports

Qiao MX,Li C,Zhang AQ,Hou LL,Yang J,Hu HG

Sat Oct 01 00:00:00 UTC 2016

DEK over-expression promotes mitotic defects and micronucleus formation.

Cell cycle (Georgetown, Tex.)

Matrka MC,Hennigan RF,Kappes F,DeLay ML,Lambert PF,Aronow BJ,Wells SI

Mon Sep 26 00:00:00 UTC 2016

DEK over-expression promotes mitotic defects and micronucleus formation.

Cell cycle (Georgetown, Tex.)

Matrka MC,Hennigan RF,Kappes F,DeLay ML,Lambert PF,Aronow BJ,Wells SI

Mon Sep 26 00:00:00 UTC 2016

Hira-mediated H3.3 incorporation is required for DNA replication and ribosomal RNA transcription in the mouse zygote.

Developmental cell

Lin CJ,Koh FM,Wong P,Conti M,Ramalho-Santos M

Mon Aug 11 00:00:00 UTC 2014

Hira-mediated H3.3 incorporation is required for DNA replication and ribosomal RNA transcription in the mouse zygote.

Developmental cell

Lin CJ,Koh FM,Wong P,Conti M,Ramalho-Santos M

Mon Aug 11 00:00:00 UTC 2014

DEK depletion negatively regulates Rho/ROCK/MLC pathway in non-small cell lung cancer.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Wang J,Sun L,Yang M,Luo W,Gao Y,Liu Z,Qiu X,Wang E

Mon Jul 01 00:00:00 UTC 2013

DEK depletion negatively regulates Rho/ROCK/MLC pathway in non-small cell lung cancer.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Wang J,Sun L,Yang M,Luo W,Gao Y,Liu Z,Qiu X,Wang E

Mon Jul 01 00:00:00 UTC 2013

DEK depletion negatively regulates Rho/ROCK/MLC pathway in non-small cell lung cancer.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Wang J,Sun L,Yang M,Luo W,Gao Y,Liu Z,Qiu X,Wang E

Mon Jul 01 00:00:00 UTC 2013

DEK depletion negatively regulates Rho/ROCK/MLC pathway in non-small cell lung cancer.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Wang J,Sun L,Yang M,Luo W,Gao Y,Liu Z,Qiu X,Wang E

Mon Jul 01 00:00:00 UTC 2013

Maintenance of muscle stem-cell quiescence by microRNA-489.

Nature

Cheung TH,Quach NL,Charville GW,Liu L,Park L,Edalati A,Yoo B,Hoang P,Rando TA

Thu Feb 23 00:00:00 UTC 2012

Maintenance of muscle stem-cell quiescence by microRNA-489.

Nature

Cheung TH,Quach NL,Charville GW,Liu L,Park L,Edalati A,Yoo B,Hoang P,Rando TA

Thu Feb 23 00:00:00 UTC 2012

The histone chaperone Spt6 is required for activation-induced cytidine deaminase target determination through H3K4me3 regulation.

The Journal of biological chemistry

Begum NA,Stanlie A,Nakata M,Akiyama H,Honjo T

Fri Sep 21 00:00:00 UTC 2012

The histone chaperone Spt6 is required for activation-induced cytidine deaminase target determination through H3K4me3 regulation.

The Journal of biological chemistry

Begum NA,Stanlie A,Nakata M,Akiyama H,Honjo T

Fri Sep 21 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: DEK oncogene (DNA binding); Protein DEK

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Gene Aliases: 1810019E15Rik; D13H6S231E; D6S231E; DEK

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UniProt ID: (Human) P35659, (Rat) Q6AXS3, (Mouse) Q7TNV0

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Entrez Gene ID: (Human) 7913, (Rat) 306817, (Mouse) 110052

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DNA binding histone binding poly(A) RNA binding molecular_function

biological_process chromatin modification regulation of double-strand break repair regulation of double-strand break repair via nonhomologous end joining regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter signal transduction covalent chromatin modification viral genome replication positive regulation of gene expression, epigenetic cell quiescence

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