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Invitrogen
DENND5A Recombinant Rabbit Monoclonal Antibody (14H6L8)
FIGURE: 1 / 3
DENND5A Antibody (702789) in WB
Western blot analysis was performed on Membrane enriched extracts (30µg lysate) of IMR-32 (Lane 1), SH-SY5Y (Lane 2), SK-N-AS (Lane 3), PC12 (Lane 4) and tissue extracts of Mouse Brain (Lane 5) and Rat Brain (Lane6). The blots were probed with Anti-DENND5A Recombinant Rabbit Monoclonal Antibody (Product # 702789, 1:5,000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:4,000 dilution). A 160 kDa band corresponding to DENND5A was observed... View More
Product Details
702789
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
14H6L8
Immunogen
Peptide corresponding to human DENND5A [aa828-aa847]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2734803
Product Specific Information
This antibody is predicted to react with Monkey, Mouse, Rat.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
DENND5A (DENN domain-containing protein 5A) is a guanine nucleotide exchange factor (GEF) which activates RAB6A and RAB39A/RAB39B. It promotes the exchange of GDP to GTP by converting inactive GDP-bound Rab proteins to their active GTP form. DENND5A is also involved in the negative regulation of neurtie outgrowth. Mutations affecting this gene can result in epileptic encephalopathy, early infantile, 49 (EIEE49).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DENN domain-containing protein 5A; DENN/MADD domain containing 5A; RAB6 interacting protein 1; Rab6-interacting protein 1; Rab6IP1
Gene Aliases: 1500012B19Rik; AJ245569; AW496491; DENND5A; KIAA1091; mKIAA1091; ORF37; RAB6IP1
UniProt ID: (Human) Q6IQ26, (Mouse) Q6PAL8, (Rat) G3V7Q0
Entrez Gene ID: (Human) 23258, (Mouse) 19347, (Rat) 308942
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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