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Proteintech
DFF45/DFFA Polyclonal Antibody
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Product Details
10191-2-AP
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
DFF45/DFFA Fusion Protein Ag0244 (8-328 aa encoded by BC000037)
Conjugate
Form
Liquid
Concentration
0.4 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Product Specific Information
Immunogen sequence: GVPESGEIR TLKPCLLRRN YSREQHGVAA SCLEDLRSKA CDILAIDKSL TPVTLVLAED GTIVDDDDYF LCLPSNTKFV ALASNEKWAY NNSDGGTAWI SQESFDVDET DSGAGLKWKN VARQLKEDLS SIILLSEEDL QMLVDAPCSD LAQELRQSCA TVQRLQHTLQ QVLDQREEVR QSKQLLQLYL QALEKEGSLL SKQEESKAAF GEEVDAVDTG ISRETSSDVA LASHILTALR EKQAPELSLS SQDLELVTKE DPKALAVALN WDIKKTETVQ EACERELALR LQQTQSLHSL RSISASKASP PGDLQNPKRA RQ (8-328 aa encoded by BC000037)
Target Information
ICAD is the inhibitor of caspase-3-activated DNase which is a substrate that controls nuclear apoptosis. ICAD has two isoforms: a functional isoform of 45kDa, ICAD-L/DNA fragmentation factor (DFF) 45; and a 35kDa isoform, ICAD-S/DFF35. Although both ICAD-L and ICAD-S can bind and inhibit CAD, only ICAD-L was reported to be functional. ICAD is cleaved to be inactivated and allow caspase-activated DNase (CAD) to execute nuclear internucleosomal apoptotic DNA fragmentation. In non-apoptotic cells, CAD is complexed with its inhibitor, ICAD. The activation of the CAD/ICAD complex occurs through the caspase 3-mediated cleavage of ICAD at residues 117 and 224, which results in three ICAD fragments that are then released from CAD. In addition to its DNase inhibitory activity, ICAD acts as a CAD specific folding chaperone. There are recent reports that ICAD is a potential target for restoring a normal apoptotic signal transduction pathway in colon and brain cancer cells. Two alternatively spliced transcript variants encoding distinct isoforms have been found for the ICAD gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DFF; DFF-45; DFF45; DNA fragmentation factor 45 kDa subunit; DNA fragmentation factor subunit alpha; DNA fragmentation factor, 45kDa, alpha polypeptide; ICAD; Inhibitor of CAD; RP23-121D17.3
Gene Aliases: DFF-45; DFF1; DFF45; DFFA; H13; ICAD
UniProt ID: (Human) O00273
Entrez Gene ID: (Human) 1676
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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