Proteintech

DGKA Polyclonal Antibody

DGKA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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DGKA Antibody (11547-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 11547-1-AP ( DGKA antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

DGKA Antibody in Immunoprecipitation (IP)

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Product Details

11547-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 14 publications 14 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

DGKA Fusion Protein Ag2075 (115-501 aa encoded by BC023523)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: LEFTFKLYD TDRNGILDSS EVDKIILQMM RVAEYLDWDV SELRPILQEM MKEIDYDGSG SVSQAEWVRA GATTVPLLVL LGLEMTLKDD GQHMWRPKRF PRPVYCNLCE SSIGLGKQGL SCNLCKYTVH DQCAMKALPC EVSTYAKSRK DIGVQSHVWV RGGCESGRCD RCQKKIRIYH SLTGLHCVWC HLEIHDDCLQ AVGHECDCGL LRDHILPPSS IYPSVLASGP DRKNSKTSQK TMDDLNLSTS EALRIDPVPN THPLLVFVNP KSGGKQGQRV LWKFQYILNP RQVFNLLKDG PEIGLRLFKD VPDSRILVCG GDGTVGWILE TIDKANLPVL PPVAVLPLGT GNDLARCLRW GGGYEGQNLA KILKDLEMSK VVHMDRWS (115-501 aa encoded by BC023523)

Target Information

The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Wiskott-Aldrich syndrome protein interacts and inhibits diacylglycerol kinase alpha promoting IL-2 induction.

Frontiers in immunology

Velnati S,Centonze S,Rossino G,Purghè B,Antona A,Racca L,Mula S,Ruffo E,Malacarne V,Malerba M,Manfredi M,Graziani A,Baldanzi G

Fri May 19 00:00:00 UTC 2023

Wiskott-Aldrich syndrome protein interacts and inhibits diacylglycerol kinase alpha promoting IL-2 induction.

Frontiers in immunology

Velnati S,Centonze S,Rossino G,Purghè B,Antona A,Racca L,Mula S,Ruffo E,Malacarne V,Malerba M,Manfredi M,Graziani A,Baldanzi G

Fri May 19 00:00:00 UTC 2023

Targeting diacylglycerol kinase α impairs lung tumorigenesis by inhibiting cyclin D3.

Thoracic cancer

Zhou D,Liu T,Rao X,Jie X,Chen Y,Wu Z,Deng H,Zhang D,Wang J,Wu G

Mon May 01 00:00:00 UTC 2023

Targeting diacylglycerol kinase α impairs lung tumorigenesis by inhibiting cyclin D3.

Thoracic cancer

Zhou D,Liu T,Rao X,Jie X,Chen Y,Wu Z,Deng H,Zhang D,Wang J,Wu G

Mon May 01 00:00:00 UTC 2023

Combination therapy for hepatocellular carcinoma with diacylglycerol kinase alpha inhibition and anti-programmed cell death-1 ligand blockade.

Cancer immunology, immunotherapy : CII

Okada N,Sugiyama K,Shichi S,Shirai Y,Goto K,Sakane F,Kitamura H,Taketomi A

Fri Apr 01 00:00:00 UTC 2022

Selective inhibitors of bromodomain BD1 and BD2 of BET proteins modulate radiation-induced profibrotic fibroblast responses.

International journal of cancer

Liu CS,Rioja I,Bakr A,Veldwijk MR,Sperk E,Herskind C,Weichenhan D,Prinjha RK,Plass C,Schmezer P,Popanda O

Fri Jul 15 00:00:00 UTC 2022

Loss of Diacylglycerol Kinase α Enhances Macrophage Responsiveness.

Frontiers in immunology

Manigat LC,Granade ME,Taori S,Miller CA,Vass LR,Zhong XP,Harris TE,Purow BW

Fri Jan 07 00:00:00 UTC 2022

Diacylglycerol kinase α inhibition cooperates with PD-1-targeted therapies to restore the T cell activation program.

Cancer immunology, immunotherapy : CII

Arranz-Nicolás J,Martin-Salgado M,Adán-Barrientos I,Liébana R,Del Carmen Moreno-Ortíz M,Leitner J,Steinberger P,Ávila-Flores A,Merida I

Mon Nov 01 00:00:00 UTC 2021

Diacylglycerol kinase ζ promotes actin cytoskeleton remodeling and mechanical forces at the B cell immune synapse.

Science signaling

Merino-Cortés SV,Gardeta SR,Roman-Garcia S,Martínez-Riaño A,Pineau J,Liebana R,Merida I,Dumenil AL,Pierobon P,Husson J,Alarcon B,Carrasco YR

Tue Apr 14 00:00:00 UTC 2020

DGKA Provides Platinum Resistance in Ovarian Cancer Through Activation of c-JUN-WEE1 Signaling.

Clinical cancer research : an official journal of the American Association for Cancer Research

Li J,Pan C,Boese AC,Kang J,Umano AD,Magliocca KR,Yang W,Zhang Y,Lonial S,Jin L,Kang S

Wed Jul 15 00:00:00 UTC 2020

DGKA Provides Platinum Resistance in Ovarian Cancer Through Activation of c-JUN-WEE1 Signaling.

Clinical cancer research : an official journal of the American Association for Cancer Research

Li J,Pan C,Boese AC,Kang J,Umano AD,Magliocca KR,Yang W,Zhang Y,Lonial S,Jin L,Kang S

Wed Jul 15 00:00:00 UTC 2020

Reprogramming fatty acyl specificity of lipid kinases via C1 domain engineering.

Nature chemical biology

Ware TB,Franks CE,Granade ME,Zhang M,Kim KB,Park KS,Gahlmann A,Harris TE,Hsu KL

Sat Feb 01 00:00:00 UTC 2020

Diacylglycerol kinase ζ limits IL-2-dependent control of PD-1 expression in tumor-infiltrating T lymphocytes.

Journal for immunotherapy of cancer

Arranz-Nicolás J,Martin-Salgado M,Rodríguez-Rodríguez C,Liébana R,Moreno-Ortiz MC,Leitner J,Steinberger P,Ávila-Flores A,Merida I

Sun Nov 01 00:00:00 UTC 2020

Mutant p53s generate pro-invasive niches by influencing exosome podocalyxin levels.

Nature communications

Novo D,Heath N,Mitchell L,Caligiuri G,MacFarlane A,Reijmer D,Charlton L,Knight J,Calka M,McGhee E,Dornier E,Sumpton D,Mason S,Echard A,Klinkert K,Secklehner J,Kruiswijk F,Vousden K,Macpherson IR,Blyth K,Bailey P,Yin H,Carlin LM,Morton J,Zanivan S,Norman JC

Thu Nov 29 00:00:00 UTC 2018

Mutant p53s generate pro-invasive niches by influencing exosome podocalyxin levels.

Nature communications

Thu Nov 29 00:00:00 UTC 2018

Decreased LIPF expression is correlated with DGKA and predicts poor outcome of gastric cancer.

Oncology reports

Kong Y,Zheng Y,Jia Y,Li P,Wang Y

Sat Oct 01 00:00:00 UTC 2016

Decreased LIPF expression is correlated with DGKA and predicts poor outcome of gastric cancer.

Oncology reports

Kong Y,Zheng Y,Jia Y,Li P,Wang Y

Sat Oct 01 00:00:00 UTC 2016

Epigenetic regulation of diacylglycerol kinase alpha promotes radiation-induced fibrosis.

Nature communications

Weigel C,Veldwijk MR,Oakes CC,Seibold P,Slynko A,Liesenfeld DB,Rabionet M,Hanke SA,Wenz F,Sperk E,Benner A,Rösli C,Sandhoff R,Assenov Y,Plass C,Herskind C,Chang-Claude J,Schmezer P,Popanda O

Fri Mar 11 00:00:00 UTC 2016

Epigenetic regulation of diacylglycerol kinase alpha promotes radiation-induced fibrosis.

Nature communications

Weigel C,Veldwijk MR,Oakes CC,Seibold P,Slynko A,Liesenfeld DB,Rabionet M,Hanke SA,Wenz F,Sperk E,Benner A,Rösli C,Sandhoff R,Assenov Y,Plass C,Herskind C,Chang-Claude J,Schmezer P,Popanda O

Fri Mar 11 00:00:00 UTC 2016

Diacylglycerol kinase α is a critical signaling node and novel therapeutic target in glioblastoma and other cancers.

Cancer discovery

Dominguez CL,Floyd DH,Xiao A,Mullins GR,Kefas BA,Xin W,Yacur MN,Abounader R,Lee JK,Wilson GM,Harris TE,Purow BW

Mon Jul 01 00:00:00 UTC 2013

Diacylglycerol kinase α is a critical signaling node and novel therapeutic target in glioblastoma and other cancers.

Cancer discovery

Dominguez CL,Floyd DH,Xiao A,Mullins GR,Kefas BA,Xin W,Yacur MN,Abounader R,Lee JK,Wilson GM,Harris TE,Purow BW

Mon Jul 01 00:00:00 UTC 2013

A miR-297/hypoxia/DGK-α axis regulating glioblastoma survival.

Neuro-oncology

Kefas B,Floyd DH,Comeau L,Frisbee A,Dominguez C,Dipierro CG,Guessous F,Abounader R,Purow B

Sun Dec 01 00:00:00 UTC 2013

A miR-297/hypoxia/DGK-α axis regulating glioblastoma survival.

Neuro-oncology

Kefas B,Floyd DH,Comeau L,Frisbee A,Dominguez C,Dipierro CG,Guessous F,Abounader R,Purow B

Sun Dec 01 00:00:00 UTC 2013

Diacylglycerol kinase regulates tyrosinase expression and function in human melanocytes.

The Journal of investigative dermatology

Kawaguchi M,Valencia JC,Namiki T,Suzuki T,Hearing VJ

Sat Dec 01 00:00:00 UTC 2012

Diacylglycerol kinase regulates tyrosinase expression and function in human melanocytes.

The Journal of investigative dermatology

Kawaguchi M,Valencia JC,Namiki T,Suzuki T,Hearing VJ

Sat Dec 01 00:00:00 UTC 2012

Biosynthesis of alkyl lysophosphatidic acid by diacylglycerol kinases.

Biochemical and biophysical research communications

Gellett AM,Kharel Y,Sunkara M,Morris AJ,Lynch KR

Fri Jun 15 00:00:00 UTC 2012

Biosynthesis of alkyl lysophosphatidic acid by diacylglycerol kinases.

Biochemical and biophysical research communications

Gellett AM,Kharel Y,Sunkara M,Morris AJ,Lynch KR

Fri Jun 15 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: 80 kDa diacylglycerol kinase; DAG kinase alpha; DGK-alpha; Diacylglycerol kinase alpha; diacylglycerol kinase alpha Flt2-Pr2; diacylglycerol kinase, alpha 80kDa; Diglyceride kinase; Diglyceride kinase alpha; MGC12821; MGC42356; truncated diacylglycerol kinase alpha Flt1-Pr1; truncated diacylglycerol kinase alpha Flt1-Pr2

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Gene Aliases: 80kDa; AW146112; DAGK; DAGK1; DGK-alpha; DGKA

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UniProt ID: (Human) P23743, (Mouse) O88673, (Rat) P51556

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Entrez Gene ID: (Human) 1606, (Mouse) 13139, (Rat) 140866

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Function(s)

diacylglycerol kinase activity calcium ion binding ATP binding phospholipid binding nucleotide binding kinase activity transferase activity metal ion binding NAD+ kinase activity

Process(es)

phosphatidic acid biosynthetic process protein kinase C-activating G-protein coupled receptor signaling pathway phosphorylation platelet activation intracellular signal transduction

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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DGKA Polyclonal Antibody

DGKA Antibody (11547-1-AP) in IHC (P)

Product Details

11547-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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