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DGKE Antibody (PA5-36250) in IHC (P)
Immunohistochemistry analysis of DGKE in paraffin-embedded human rectum carcinoma tissue (cytoplasmic staining) and negative control (right, with PBS only). Samples were incubated with DGKE polyclonal antibody (Product # PA5-36250) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.
Product Details
PA5-36250
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to amino acids 180-230 of human DGK- epsilon
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2553440
Product Specific Information
This antibody detects endogenous protein at a molecular weight of 64 kDa.
Purity is >95% by SDS-PAGE.
Target Information
Diacylglycerol (DAG) influences numerous cell signaling cascades by functioning as an intracellular, allosteric activator of protein kinase C (PKC), and as a potent activator of guanine nucleotide exchange factors. In order to maintain cellular homeostasis, intracellular DAG levels are tightly regulated by diacylglycerol kinases (DGKs, DAGKs), which phosphorylate DAG to phosphatidic acid, thus removing DAG. Human DGK-alpha (80 kDa), -beta (90 kDa), and - gamma (90 kDa) have calcium-binding EF-hand motifs at their N termini and are classified as type I DGKs. Human DGK- delta (130 kDa) and DGK-i (130 kDa) contain N-terminal pleckstrin homology (PH) domains and are classified as type II. Human DGK- epsilon (64 kDa) contains no identifiable regulatory domains and is classified as a type III DGK. Human DGK- zeta (104 kDa) and -iota (130 kDa) possess C-terminal ankyrin repeats and are classified as type IV DGKs. Human DGK-theta (110 kDa) contains 3 cysteine-rich domains and a PH domain and is classified as a type V DGK.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DAG kinase epsilon; DGK-epsilon; Diacylglycerol kinase epsilon; diacylglycerol kinase, epsilon 64kDa; Diglyceride kinase epsilon; EC 2.7.1.107
Gene Aliases: AHUS7; C87606; DAGK5; DAGK6; DGK; DGKE; NPHS7
UniProt ID: (Human) P52429, (Mouse) Q9R1C6
Entrez Gene ID: (Human) 8526, (Mouse) 56077, (Rat) 497978
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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