Proteintech

DHFR Polyclonal Antibody

DHFR Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 13

DHFR Antibody (15194-1-AP) in ICC/IF

Immunofluorescent analysis of HeLa cells using 15194-1-AP (DHFR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

DHFR Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DHFR Antibody in Immunoprecipitation (IP)

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Product Details

15194-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 16 publications 16 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

DHFR Fusion Protein Ag7331 (1-187 aa encoded by BC000192)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MVGSLNCIV AVSQNMGIGK NGDLPWPPLR NEFRYFQRMT TTSSVEGKQN LVIMGKKTWF SIPEKNRPLK GRINLVLSRE LKEPPQGAHF LSRSLDDALK LTEQPELANK VDMVWIVGGS SVYKEAMNHP GHLKLFVTRI MQDFESDTFF PEIDLEKYKL LPEYPGVLSD VQEEKGIKYK FEVYEKND (1-187 aa encoded by BC000192)

Target Information

Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, and is required for the synthesis of purines, thymidylic acid and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Disease-linked mutations cause exposure of a protein quality control degron.

Structure (London, England : 1993)

Kampmeyer C,Larsen-Ledet S,Wagnkilde MR,Michelsen M,Iversen HKM,Nielsen SV,Lindemose S,Caregnato A,Ravid T,Stein A,Teilum K,Lindorff-Larsen K,Hartmann-Petersen R

Thu Sep 01 00:00:00 UTC 2022

ENO1 suppresses cancer cell ferroptosis by degrading the mRNA of iron regulatory protein 1.

Nature cancer

Zhang T,Sun L,Hao Y,Suo C,Shen S,Wei H,Ma W,Zhang P,Wang T,Gu X,Li ST,Chen Z,Yan R,Zhang Y,Cai Y,Zhou R,Jia W,Huang F,Gao P,Zhang H

Sat Jan 01 00:00:00 UTC 2022

Proteasome granule formation is regulated through mitochondrial respiration and kinase signaling.

Journal of cell science

Waite KA,Roelofs J

Thu Sep 01 00:00:00 UTC 2022

A Proximal-to-Distal Survey of Healthy Adult Human Small Intestine and Colon Epithelium by Single-Cell Transcriptomics.

Cellular and molecular gastroenterology and hepatology

Burclaff J,Bliton RJ,Breau KA,Ok MT,Gomez-Martinez I,Ranek JS,Bhatt AP,Purvis JE,Woosley JT,Magness ST

Mon Apr 18 00:00:00 UTC 2022

Metformin sensitises hepatocarcinoma cells to methotrexate by targeting dihydrofolate reductase.

Cell death & disease

Wang Y,Lu H,Sun L,Chen X,Wei H,Suo C,Feng J,Yuan M,Shen S,Jia W,Wang Y,Zhang H,Li Z,Zhong X,Gao P

Sat Oct 02 00:00:00 UTC 2021

Metformin sensitises hepatocarcinoma cells to methotrexate by targeting dihydrofolate reductase.

Cell death & disease

Wang Y,Lu H,Sun L,Chen X,Wei H,Suo C,Feng J,Yuan M,Shen S,Jia W,Wang Y,Zhang H,Li Z,Zhong X,Gao P

Sat Oct 02 00:00:00 UTC 2021

Establishment of a Dihydrofolate Reductase Gene Knock-In Zebrafish Strain to Aid Preliminary Analysis of Congenital Heart Disease Mechanisms.

Frontiers in cardiovascular medicine

Gong K,Xie T,Yang Y,Luo Y,Deng Y,Chen K,Tan Z,Guo H,Xie L

Tue Jan 04 00:00:00 UTC 2022

mTORC1 stimulates cell growth through SAM synthesis and m⁶A mRNA-dependent control of protein synthesis.

Molecular cell

Villa E,Sahu U,O'Hara BP,Ali ES,Helmin KA,Asara JM,Gao P,Singer BD,Ben-Sahra I

Thu May 20 00:00:00 UTC 2021

Distinct Classes of Flavonoids and Epigallocatechin Gallate, Polyphenol Affects an Oncogenic Mutant p53 Protein, Cell Growth and Invasion in a TNBC Breast Cancer Cell Line.

Cells

Kollareddy M,Martinez LA

Fri Apr 02 00:00:00 UTC 2021

Thymidylate synthase inhibitor raltitrexed can induce high levels of DNA damage in MYCN-amplified neuroblastoma cells.

Cancer science

Yamashita K,Kiyonari S,Tsubota S,Kishida S,Sakai R,Kadomatsu K

Wed Jul 01 00:00:00 UTC 2020

A Simplified Thermal Proteome Profiling Approach to Screen Protein Targets of a Ligand.

Proteomics

Zhang X,Ruan C,Zhu H,Li K,Zhang W,Wang K,Hu L,Ye M

Sat Aug 01 00:00:00 UTC 2020

Pemetrexed inhibits Kaposi's sarcoma-associated herpesvirus replication through blocking dTMP synthesis.

Antiviral research

Chen J,Zhang H,Chen X

Sat Aug 01 00:00:00 UTC 2020

CXCR4 regulates Plasmodium development in mouse and human hepatocytes.

The Journal of experimental medicine

Bando H,Pradipta A,Iwanaga S,Okamoto T,Okuzaki D,Tanaka S,Vega-Rodríguez J,Lee Y,Ma JS,Sakaguchi N,Soga A,Fukumoto S,Sasai M,Matsuura Y,Yuda M,Jacobs-Lorena M,Yamamoto M

Mon Aug 05 00:00:00 UTC 2019

Establishment of DHFR-deficient HEK293 cells for high yield of therapeutic glycoproteins.

Journal of bioscience and bioengineering

Mensah EO,Guo XY,Gao XD,Fujita M

Tue Oct 01 00:00:00 UTC 2019

The protective effect of polyunsaturated fatty acid intake during pregnancy against embryotoxicity of sodium valproate in mice.

Food & function

Li K,Li J,Gu J,Guo X,Gao T,Li D

Wed May 23 00:00:00 UTC 2018

'Rod and ring' formation from IMP dehydrogenase is regulated through the one-carbon metabolic pathway.

Journal of cell science

Calise SJ,Purich DL,Nguyen T,Saleem DA,Krueger C,Yin JD,Chan EK

Mon Aug 01 00:00:00 UTC 2016

'Rod and ring' formation from IMP dehydrogenase is regulated through the one-carbon metabolic pathway.

Journal of cell science

Calise SJ,Purich DL,Nguyen T,Saleem DA,Krueger C,Yin JD,Chan EK

Mon Aug 01 00:00:00 UTC 2016

'Rod and ring' formation from IMP dehydrogenase is regulated through the one-carbon metabolic pathway.

Journal of cell science

Calise SJ,Purich DL,Nguyen T,Saleem DA,Krueger C,Yin JD,Chan EK

Mon Aug 01 00:00:00 UTC 2016

'Rod and ring' formation from IMP dehydrogenase is regulated through the one-carbon metabolic pathway.

Journal of cell science

Calise SJ,Purich DL,Nguyen T,Saleem DA,Krueger C,Yin JD,Chan EK

Mon Aug 01 00:00:00 UTC 2016

Down-regulation of dihydrofolate reductase inhibits the growth of endothelial EA.hy926 cell through induction of G1 cell cycle arrest via up-regulating p53 and p21(waf1/cip1) expression.

Journal of clinical biochemistry and nutrition

Fei Z,Gao Y,Qiu M,Qi X,Dai Y,Wang S,Quan Z,Liu Y,Ou J

Tue Mar 01 00:00:00 UTC 2016

Aspirin antagonizes the cytotoxic effect of methotrexate in lung cancer cells.

Oncology reports

Yan KH,Lee LM,Hsieh MC,Yan MD,Yao CJ,Chang PY,Chen TL,Chang HY,Cheng AL,Lai GM,Chuang SE

Sun Sep 01 00:00:00 UTC 2013

Aspirin antagonizes the cytotoxic effect of methotrexate in lung cancer cells.

Oncology reports

Yan KH,Lee LM,Hsieh MC,Yan MD,Yao CJ,Chang PY,Chen TL,Chang HY,Cheng AL,Lai GM,Chuang SE

Sun Sep 01 00:00:00 UTC 2013

Identification and characterization of an inborn error of metabolism caused by dihydrofolate reductase deficiency.

American journal of human genetics

Banka S,Blom HJ,Walter J,Aziz M,Urquhart J,Clouthier CM,Rice GI,de Brouwer AP,Hilton E,Vassallo G,Will A,Smith DE,Smulders YM,Wevers RA,Steinfeld R,Heales S,Crow YJ,Pelletier JN,Jones S,Newman WG

Fri Feb 11 00:00:00 UTC 2011

Identification and characterization of an inborn error of metabolism caused by dihydrofolate reductase deficiency.

American journal of human genetics

Banka S,Blom HJ,Walter J,Aziz M,Urquhart J,Clouthier CM,Rice GI,de Brouwer AP,Hilton E,Vassallo G,Will A,Smith DE,Smulders YM,Wevers RA,Steinfeld R,Heales S,Crow YJ,Pelletier JN,Jones S,Newman WG

Fri Feb 11 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: Dihydrofolate reductase; Dihydrofolate reductase 1 (active)

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Gene Aliases: 8430436I03Rik; AA607882; AI662710; AW555094; DHFR; Dhfr1; DHFRP1; DYR

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UniProt ID: (Human) P00374, (Mouse) P00375, (Rat) Q920D2

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Entrez Gene ID: (Human) 1719, (Mouse) 13361, (Rat) 24312

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Function(s)

mRNA binding dihydrofolate reductase activity folic acid binding drug binding folate reductase activity methotrexate binding NADPH binding RNA binding oxidoreductase activity NADP binding dihydrofolic acid binding

Process(es)

G1/S transition of mitotic cell cycle regulation of transcription involved in G1/S transition of mitotic cell cycle glycine biosynthetic process tetrahydrobiopterin biosynthetic process one-carbon metabolic process nucleotide biosynthetic process axon regeneration response to methotrexate dihydrofolate metabolic process tetrahydrofolate metabolic process tetrahydrofolate biosynthetic process folic acid metabolic process positive regulation of nitric-oxide synthase activity oxidation-reduction process regulation of removal of superoxide radicals response to nicotine

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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DHFR Polyclonal Antibody

DHFR Antibody (15194-1-AP) in ICC/IF

Product Details

15194-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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