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DHFR Antibody (PA5-79152) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of DHFR in U20S cells using DHFR Polyclonal Antibody (Product # PA5-79152). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL. DyLight 550 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets ... View More
Product Details
PA5-79152
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human DHFR recombinant protein (Position: V2-D187).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746268
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human Jurkat whole cell, rat liver tissue, rat kidney tissue, mouse liver tissue, mouse kidney tissue. IHC: human colorectal cancer tissue, mouse small intestine tissue, rat small intestine tissue. ICC/IF: U20S cell. Flow: PC-3 cell.
Target Information
Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, and is required for the synthesis of purines, thymidylic acid and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Dihydrofolate reductase; Dihydrofolate reductase 1 (active)
Gene Aliases: 8430436I03Rik; AA607882; AI662710; AW555094; DHFR; Dhfr1; DHFRP1; DYR
UniProt ID: (Human) P00374, (Mouse) P00375, (Rat) Q920D2
Entrez Gene ID: (Human) 1719, (Mouse) 13361, (Rat) 24312
G1/S transition of mitotic cell cycle
regulation of transcription involved in G1/S transition of mitotic cell cycle
glycine biosynthetic process
tetrahydrobiopterin biosynthetic process
one-carbon metabolic process
nucleotide biosynthetic process
axon regeneration
response to methotrexate
dihydrofolate metabolic process
tetrahydrofolate metabolic process
tetrahydrofolate biosynthetic process
folic acid metabolic process
positive regulation of nitric-oxide synthase activity
oxidation-reduction process
regulation of removal of superoxide radicals
response to nicotine
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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