Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 3
DHX9 Antibody (MA5-35534) in ICC/IF
Confocal imaging (Immunocytochemistry) of DHX9 in U-2 OS cells. Samples were incubated with DHX9 Monoclonal antibody (Product # MA5-35534) using a dilution of 1:100 (Red). The cells were counterstained with alpha Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
Product Details
MA5-35534
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
2I6C5
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1100-1200 of human DHX9/RNA Helicase A (Q08211)
Conjugate
Form
Liquid
Concentration
0.08 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2849434
Product Specific Information
Immunogen sequence: ITGLRAAMEA LVVEVTKQPA IISQLDPVNE RMLNMIRQIS RPSAAGINLM IGSTRYGDGP RPPKMARYDN GSGYRRGGSS YSGGGYGGGY SSGGYGSGGY G
Target Information
The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: ATP-dependent RNA helicase A; D-E-A-D (aspartate-glutamate-alanine-aspartate) box polypeptide 9; DEAD (aspartate-glutamate-alanine-aspartate) box polypeptide 9; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 9; DEAH (Asp-Glu-Ala-His) box helicase 9; DEAH (Asp-Glu-Ala-His) box polypeptide 9; DEAH box protein 9; DExH-box helicase 9; FLJ17406; Leukophysin; LKP; mHEL-5; NDH II; Nuclear DNA helicase II; OTTHUMP00000033287; RHA; RNA helicase; RNA helicase A
Gene Aliases: AI326842; DDX9; DHX9; HEL-5; LKP; mHEL-5; NDH2; NDHII; RHA
UniProt ID: (Human) Q08211, (Mouse) O70133
Entrez Gene ID: (Human) 1660, (Mouse) 13211, (Rat) 304859
RNA polymerase II transcription factor binding
DNA binding
RNA helicase activity
ATP-dependent DNA helicase activity
ATP-dependent RNA helicase activity
protein binding
ATP binding
poly(A) RNA binding
nucleotide binding
nucleic acid binding
RNA binding
helicase activity
ATP-dependent helicase activity
hydrolase activity
molecular_function
osteoblast differentiation
RNA processing
circadian rhythm
cellular response to heat
rhythmic process
CRD-mediated mRNA stabilization
biological_process
mRNA splicing, via spliceosome
positive regulation of type I interferon production
DNA duplex unwinding
protein localization to cytoplasmic stress granule
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support