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Invitrogen
DIAPH3 Polyclonal Antibody
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FIGURE: 1 / 7
DIAPH3 Antibody (PA5-115123) in ICC/IF
Immunocytochemistry analysis of DIAPH3 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115123) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-115123
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human DIAPH3(Accession Q9NSV4), corresponding to amino acid residues T825-S875.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2899759
Product Specific Information
Antibody detects endogenous levels of total DIAPH3.
Target Information
This gene encodes a member of the diaphanous subfamily of the formin family. Members of this family are involved in actin remodeling and regulate cell movement and adhesion. Mutations in this gene are associated with autosomal dominant auditory neuropathy 1. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: diaphanous homolog 3; diaphanous-related formin; diaphanous-related formin 3; Diaphanous-related formin-3; DKFZp434C0931; DKFZp686A13178; DRF3; FLJ34705; MDia2; p134mDIA2; Protein diaphanous homolog 3
Gene Aliases: 4930417P13Rik; AN; AUNA1; DIA2; DIAP3; DIAPH3; DRF3; mDia2; mKIAA4117; NSDAN; p134MDia2
UniProt ID: (Human) Q9NSV4, (Mouse) Q9Z207, (Rat) F1LVW7
Entrez Gene ID: (Human) 81624, (Mouse) 56419, (Rat) 290396
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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