Invitrogen

DISC1 Polyclonal Antibody

DISC1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 12

DISC1 Antibody (40-6800) in ICC/IF

Immunofluorescence analysis of DISC1 (C-TERM) was done on 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with DISC1 (C-TERM) Rabbit Polyclonal Antibody (Product # 40-6800) at 1 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (... View More

DISC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DISC1 Antibody in Immunohistochemistry (IHC)

DISC1 Antibody in Western Blot, Immunoprecipitation (WB, IP)

DISC1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

DISC1 Antibody — Figure 7 DISC1 knockdown and rescue in striatal neurons. ( A ) Example immunoblot showing depletion of endogenous DISC1 detected in PC12 cells using the C-terminal antibody (see Materials and Methods ). The immunoreactive band corresponding to the major full length DISC1 species is shown. ( B ) Example of DISC1 depletion in striatal cultures, detected using the C-terminal (C-term) antibody and verified using the midi antibody, as indicated. ( C ) Quantification of knockdown and rescue phenotypes, based on count of cilia number compiled from 8 independent experiments. ( D ) Examples of the phenotypes observed in the indicated conditions (indicated above each column). The rescue condition (right column of images) shows the localization of DISC1-GFP rather than GFP. Insets indicate relevant regions of the cell body at higher magnification. {KD}

Product Details

40-6800

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

1 µg/mL

Immunoprecipitation (IP)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from the C-terminal region of the mouse DISC1 (disrupted in schizophrenia 1) protein

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533476

Target Information

DISC1 (Disrupted in Schizophrenia 1) is a multifunctional protein that interacts with multiple proteins of the centrosome and cytoskeletal system, and is involved in neuronal functions that are dependent upon proper cytoskeletal regulation, including neuronal migration, neurite architecture and intracellular transport. Regions of the brain which express DISC1 include the hippocampus, lateral septum, amygdala, cerebral cortex, cerebellum and paraventricular hypothalamus, and loss of DISC1 function results in differences in structural organization.The DISC1 gene locus is associated with patients afflicted with schizophrenia as a result of chromosomal translocations. DISC-1 encodes a large protein predicted to contain a globular N-terminal domain and a helical C-terminal domain, both of which have the potential to form interactions with other proteins. DISC-1 interacts with proteins involved in the centrosome and cytoskeletal system, including MIPT3, MAP1A and NUDEL; proteins which localize receptors to membranes, including alpha-actinin 2, beta 4-spectrin; and proteins which transduce signals from membrane receptors, including ATF-4 and ATF-5. Therefore, DISC-1 is thought to be involved in intracellular transport, neurite architecture and/or neuronal migration, all of which are thought to be pathogenic in the schizophrenic brain. Decreased expression of DISC1 in neurons caused an accelerated rate of neuronal integration, resulting in aberrant morphological development, suggesting that DISC1 plays a role in dendritic development and synapse formation. DISC-1 is thought to be involved in intracellular transport, neurite architecture and/or neuronal migration, all of which are thought to be pathogenic in the schizophrenic brain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Knock-Down of Hippocampal DISC1 in Immune-Challenged Mice Impairs the Prefrontal-Hippocampal Coupling and the Cognitive Performance Throughout Development.

Cerebral cortex (New York, N.Y. : 1991)

Xu X,Song L,Hanganu-Opatz IL

40-6800 was used in Immunohistochemistry to fill this knowledge gap by combining in vivo electrophysiology and optogenetics with morphological and behavioral assessment of immune-challenged mice with DISC1 knock-down either in the whole brain (GE) or restricted to pyramidal neurons in hippocampal CA1 area (GHPE).

Tue Jan 05 00:00:00 UTC 2021

Transient Knock-Down of Prefrontal DISC1 in Immune-Challenged Mice Causes Abnormal Long-Range Coupling and Cognitive Dysfunction throughout Development.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Xu X,Chini M,Bitzenhofer SH,Hanganu-Opatz IL

40-6800 was used in Immunohistochemistry to assess to which extent impaired prefrontal development contributes to the early cognitive dysfunction.

Wed Feb 13 00:00:00 UTC 2019

NMDA receptors are selectively partitioned into complexes and supercomplexes during synapse maturation.

Nature communications

Frank RA,Komiyama NH,Ryan TJ,Zhu F,O'Dell TJ,Grant SG

40-6800 was used in western blot to analyze selective partitioning into complexes and supercomplexes during synapse maturation by NMDA receptors

Wed Apr 27 00:00:00 UTC 2016

Synergistic interactions between PDE4B and GSK-3: DISC1 mutant mice.

Neuropharmacology

Lipina TV,Wang M,Liu F,Roder JC

Thu Mar 01 00:00:00 UTC 2012

Synergistic interactions between PDE4B and GSK-3: DISC1 mutant mice.

Neuropharmacology

Lipina TV,Wang M,Liu F,Roder JC

Thu Mar 01 00:00:00 UTC 2012

Genetic and pharmacological evidence for schizophrenia-related Disc1 interaction with GSK-3.

Synapse (New York, N.Y.)

Lipina TV,Kaidanovich-Beilin O,Patel S,Wang M,Clapcote SJ,Liu F,Woodgett JR,Roder JC

Tue Mar 01 00:00:00 UTC 2011

Genetic and pharmacological evidence for schizophrenia-related Disc1 interaction with GSK-3.

Synapse (New York, N.Y.)

Lipina TV,Kaidanovich-Beilin O,Patel S,Wang M,Clapcote SJ,Liu F,Woodgett JR,Roder JC

Tue Mar 01 00:00:00 UTC 2011

The von Economo neurons in frontoinsular and anterior cingulate cortex in great apes and humans.

Brain structure & function

Allman JM,Tetreault NA,Hakeem AY,Manaye KF,Semendeferi K,Erwin JM,Park S,Goubert V,Hof PR

Tue Jun 01 00:00:00 UTC 2010

The von Economo neurons in frontoinsular and anterior cingulate cortex in great apes and humans.

Brain structure & function

Allman JM,Tetreault NA,Hakeem AY,Manaye KF,Semendeferi K,Erwin JM,Park S,Goubert V,Hof PR

Tue Jun 01 00:00:00 UTC 2010

DISC1 regulates primary cilia that display specific dopamine receptors.

PloS one

Marley A,von Zastrow M

Fri May 28 00:00:00 UTC 2010

DISC1 regulates primary cilia that display specific dopamine receptors.

PloS one

Marley A,von Zastrow M

Fri May 28 00:00:00 UTC 2010

DISC1 regulates primary cilia that display specific dopamine receptors.

PloS one

Marley A,von Zastrow M

Fri May 28 00:00:00 UTC 2010

DISC1 regulates primary cilia that display specific dopamine receptors.

PloS one

Marley A,von Zastrow M

Fri May 28 00:00:00 UTC 2010

Bioinformatics

Protein Aliases: Disrupted in schizophrenia 1 homolog; Disrupted in schizophrenia 1 protein; RP4-730B13.1

View more View less

Gene Aliases: C1orf136; DISC1; KIAA0457; SCZD9

View more View less

UniProt ID: (Human) Q9NRI5, (Mouse) Q811T9

View more View less

Entrez Gene ID: (Human) 27185, (Mouse) 244667

View more View less

Function(s)

protein binding kinesin binding protein complex binding protein complex scaffold

Process(es)

microtubule cytoskeleton organization neuron migration positive regulation of cell-matrix adhesion positive regulation of neuroblast proliferation multicellular organism development nervous system development protein localization regulation of neuron projection development positive regulation of neuron projection development Wnt signaling pathway cerebral cortex radially oriented cell migration cell proliferation in forebrain pyramidal neuron migration positive regulation of Wnt signaling pathway TOR signaling cellular protein localization nonmotile primary cilium assembly cilium assembly positive regulation of axon extension mitochondrial calcium ion homeostasis canonical Wnt signaling pathway regulation of dendritic spine development regulation of synapse maturation positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process response to electrical stimulus protein localization to centrosome

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

DISC1 Polyclonal Antibody

DISC1 Antibody (40-6800) in ICC/IF

Product Details

40-6800

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!