Invitrogen

DISC1 Recombinant Superclonal™ Antibody (4HCLC)

DISC1 Antibody in Immunocytochemistry (ICC/IF)

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DISC1 Antibody (710203) in ICC/IF

Immunofluorescent analysis of DISC1 was done on 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. The cells were incubated with DISC1 Recombinant Rabbit Superclonal™ Antibody (Product # 710203) at 2 µg-4 µg in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor® 488 Goat anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution ... View More

DISC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

DISC1 Antibody — Fig. 4 The TRAX/DISC1/GSK3beta (TDG) complex is involved in the protective effect of an A 2A R agonist. a , b PC12 cells were treated with an inhibitor of protein kinase A (H89; 10 muM and KT5720; 10 muM) or vehicle for 30 min and then treated with CGS (10 muM) or vehicle for 1 h ( a ) and 8 h ( b ). Cells were lysed and subjected to SDS-PAGE, followed by Western blot analysis using the anti-GSK3beta Ser9, anti-GSK3beta, anti-beta-catenin and anti-alpha-Tubulin antibodies as indicated. The amount of target protein was quantified and normalized to that of alpha-Tubulin, the loading control. These experiments were repeated three times. c - e In PC12 cells and human MSN neurons, cells were infected with lentivirus expressing GSK3beta or a constitutively active GSK3beta mutant (GSK3beta-S9A) for 3 days. Cells were treated with an A 2A R agonist (CGS21680, CGS; 10 muM) or vehicle for 1 h to activate the A 2A R and then treated with H 2 O 2 (100 muM) for 4 h. Cells were lysed and subjected to SDS-PAGE, followed by Western blot analysis using the anti-gammaH2AX, anti-alpha-Tubulin and anti-beta-Actin antibodies as indicated. The amount of target protein was quantified and normalized to that of alpha-Tubulin or beta-Actin, the loading controls. These experiments were repeated three times ( c , d ). Apoptosis was assessed by co-staining with Annexin V-Cy5 and PI for 10 min followed by flow cytometric analysis ( e ). f Protein-protein interactions were monitored by the proximity ligati {TM}

Product Details

710203

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500

Immunocytochemistry (ICC/IF)

2-4 µg

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Superclonal

Type

Antibody

Clone

4HCLC

Immunogen

Peptide corresponding to amino acids 419-428 of human disrupted in schizophrenia 1

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

AB_2532621

Product Specific Information

Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

Target Information

DISC1 (Disrupted in Schizophrenia 1) is a multifunctional protein that interacts with multiple proteins of the centrosome and cytoskeletal system, and is involved in neuronal functions that are dependent upon proper cytoskeletal regulation, including neuronal migration, neurite architecture and intracellular transport. Regions of the brain which express DISC1 include the hippocampus, lateral septum, amygdala, cerebral cortex, cerebellum and paraventricular hypothalamus, and loss of DISC1 function results in differences in structural organization.The DISC1 gene locus is associated with patients afflicted with schizophrenia as a result of chromosomal translocations. DISC-1 encodes a large protein predicted to contain a globular N-terminal domain and a helical C-terminal domain, both of which have the potential to form interactions with other proteins. DISC-1 interacts with proteins involved in the centrosome and cytoskeletal system, including MIPT3, MAP1A and NUDEL; proteins which localize receptors to membranes, including alpha-actinin 2, beta 4-spectrin; and proteins which transduce signals from membrane receptors, including ATF-4 and ATF-5. Therefore, DISC-1 is thought to be involved in intracellular transport, neurite architecture and/or neuronal migration, all of which are thought to be pathogenic in the schizophrenic brain. Decreased expression of DISC1 in neurons caused an accelerated rate of neuronal integration, resulting in aberrant morphological development, suggesting that DISC1 plays a role in dendritic development and synapse formation. DISC-1 is thought to be involved in intracellular transport, neurite architecture and/or neuronal migration, all of which are thought to be pathogenic in the schizophrenic brain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

GSK3β negatively regulates TRAX, a scaffold protein implicated in mental disorders, for NHEJ-mediated DNA repair in neurons.

Molecular psychiatry

Chien T,Weng YT,Chang SY,Lai HL,Chiu FL,Kuo HC,Chuang DM,Chern Y

710203 was used in Western Blotting to report that glycogen synthase kinase 3 beta and disrupted-in-schizophrenia 1 are two novel interacting proteins of Translin-associated protein X.

Sat Dec 01 00:00:00 UTC 2018

Resources for the Comprehensive Discovery of Functional RNA Elements.

Molecular cell

Sundararaman B,Zhan L,Blue SM,Stanton R,Elkins K,Olson S,Wei X,Van Nostrand EL,Pratt GA,Huelga SC,Smalec BM,Wang X,Hong EL,Davidson JM,Lécuyer E,Graveley BR,Yeo GW

710203 was used in RNA immunoprecipitation to develop reagents to study RNA binding proteins

Thu Mar 17 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: Disrupted in schizophrenia 1 homolog; Disrupted in schizophrenia 1 protein; RP4-730B13.1

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Gene Aliases: C1orf136; DISC1; KIAA0457; SCZD9

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UniProt ID: (Human) Q9NRI5, (Mouse) Q811T9, (Rat) Q810H6

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Entrez Gene ID: (Human) 27185, (Mouse) 244667, (Rat) 307940

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Function(s)

protein binding kinesin binding protein complex binding protein complex scaffold cytoskeletal protein binding

Process(es)

microtubule cytoskeleton organization neuron migration positive regulation of cell-matrix adhesion positive regulation of neuroblast proliferation multicellular organism development nervous system development protein localization regulation of neuron projection development positive regulation of neuron projection development Wnt signaling pathway cerebral cortex radially oriented cell migration cell proliferation in forebrain pyramidal neuron migration positive regulation of Wnt signaling pathway TOR signaling cellular protein localization nonmotile primary cilium assembly cilium assembly positive regulation of axon extension mitochondrial calcium ion homeostasis canonical Wnt signaling pathway regulation of dendritic spine development regulation of synapse maturation positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process response to electrical stimulus protein localization to centrosome

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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DISC1 Recombinant Superclonal™ Antibody (4HCLC)

DISC1 Antibody (710203) in ICC/IF

Product Details

710203

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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