Proteintech

DKK3 Polyclonal Antibody

DKK3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 17

DKK3 Antibody (10365-1-AP) in ICC/IF

Immunofluorescent analysis of SH-SY5Y cells using 10365-1-AP (DKK3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

DKK3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Product Details

10365-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:20-1:200

View 5 publications 5 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Miscellaneous PubMed (MISC)

View 2 publications 2 publications

Not applicable (N/A)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Dog, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

DKK3 Fusion Protein Ag0417 (37-350 aa encoded by BC007660)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.39 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: ALSYPQEEA TLNEMFREVE ELMEDTQHKL RSAVEEMEAE EAAAKASSEV NLANLPPSYH NETNTDTKVG NNTIHVHREI HKITNNQTGQ MVFSETVITS VGDEEGRRSH ECIIDEDCGP SMYCQFASFQ YTCQPCRGQR MLCTRDSECC GDQLCVWGHC TKMATRGSNG TICDNQRDCQ PGLCCAFQRG LLFPVCTPLP VEGELCHDPA SRLLDLITWE LEPDGALDRC PCASGLLCQP HSHSLVYVCK PTFVGSRDQD GEILLPREVP DEYEVGSFME EVRQELEDLE RSLTEEMALG EPAAAAAALL GGEEI (37-350 aa encoded by B C007660)

Target Information

DKK3, like DKK1, DKK2, and DKK4, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis revealed wide expression of the DKK3 transcript, with highest expression in heart, brain, and spinal cord. In situ hybridization reveals highest expression in mouse brain, eye, and heart.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Immuno-histochemical correlation of fibrosis-related markers with the desmoplastic reaction of the mesentery in small intestine neuroendocrine neoplasms.

Journal of cancer research and clinical oncology

Bösch F,Altendorf-Hofmann A,Koliogiannis V,Ilhan H,Jacob S,Pretzsch E,Nölting S,Werner J,Klauschen F,Auernhammer CJ,Angele MK,Knösel T

Mon May 01 00:00:00 UTC 2023

Deep Sc-RNA sequencing decoding the molecular dynamic architecture of the human retina.

Science China. Life sciences

Huang L,Li R,Ye L,Zhang S,Tian H,Du M,Qu C,Li S,Li J,Yang M,Wu B,Chen R,Huang G,Zhong L,Yang H,Yu M,Shi Y,Wang C,Zhang H,Chen W,Yang Z

Wed Mar 01 00:00:00 UTC 2023

DKK3/CKAP4 axis is associated with advanced stage and poorer prognosis in oral cancer.

Oral diseases

Katase N,Kudo K,Ogawa K,Sakamoto Y,Nishimatsu SI,Yamauchi A,Fujita S

Wed Nov 01 00:00:00 UTC 2023

Retinoic acid effects on in vitro palatal fusion and WNT signaling.

European journal of oral sciences

Roa Fuentes LA,Bloemen M,Carels CE,Wagener FA,Von den Hoff JW

Thu Dec 01 00:00:00 UTC 2022

Single-cell multimodal analysis identifies common regulatory programs in synovial fibroblasts of rheumatoid arthritis patients and modeled TNF-driven arthritis.

Genome medicine

Armaka M,Konstantopoulos D,Tzaferis C,Lavigne MD,Sakkou M,Liakos A,Sfikakis PP,Dimopoulos MA,Fousteri M,Kollias G

Tue Jul 26 00:00:00 UTC 2022

MicroRNA miR-425 promotes tumor progression by inhibiting Dickkopf-related protein-3 in gastric cancer.

Bioengineered

Pei Y,Tang Z,Cai M,Yao Q,Xie B

Wed Dec 01 00:00:00 UTC 2021

Exercise ameliorates insulin resistance and improves ASK1-mediated insulin signalling in obese rats.

Journal of cellular and molecular medicine

Zhang Y,Ye T,Zhou P,Li R,Liu Z,Xie J,Hua T,Sun Q

Wed Dec 01 00:00:00 UTC 2021

Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function.

Nature cell biology

Wang L,Yu P,Zhou B,Song J,Li Z,Zhang M,Guo G,Wang Y,Chen X,Han L,Hu S

Wed Jan 01 00:00:00 UTC 2020

miR-1303 promotes the proliferation, migration and invasion of prostate cancer cells through regulating the Wnt/β-catenin pathway by targeting DKK3.

Experimental and therapeutic medicine

Liu B,Zhou W,Jiang H,Xiang Z,Wang L

Sun Dec 01 00:00:00 UTC 2019

miR-1303 promotes the proliferation, migration and invasion of prostate cancer cells through regulating the Wnt/β-catenin pathway by targeting DKK3.

Experimental and therapeutic medicine

Liu B,Zhou W,Jiang H,Xiang Z,Wang L

Sun Dec 01 00:00:00 UTC 2019

Bone secreted factors induce cellular quiescence in prostate cancer cells.

Scientific reports

Yu-Lee LY,Lee YC,Pan J,Lin SC,Pan T,Yu G,Hawke DH,Pan BF,Lin SH

Mon Dec 09 00:00:00 UTC 2019

Suppression of stromal-derived Dickkopf-3 (DKK3) inhibits tumor progression and prolongs survival in pancreatic ductal adenocarcinoma.

Science translational medicine

Zhou L,Husted H,Moore T,Lu M,Deng D,Liu Y,Ramachandran V,Arumugam T,Niehrs C,Wang H,Chiao P,Ling J,Curran MA,Maitra A,Hung MC,Lee JE,Logsdon CD,Hwang RF

Wed Oct 24 00:00:00 UTC 2018

Suppression of stromal-derived Dickkopf-3 (DKK3) inhibits tumor progression and prolongs survival in pancreatic ductal adenocarcinoma.

Science translational medicine

Zhou L,Husted H,Moore T,Lu M,Deng D,Liu Y,Ramachandran V,Arumugam T,Niehrs C,Wang H,Chiao P,Ling J,Curran MA,Maitra A,Hung MC,Lee JE,Logsdon CD,Hwang RF

Wed Oct 24 00:00:00 UTC 2018

Suppression of stromal-derived Dickkopf-3 (DKK3) inhibits tumor progression and prolongs survival in pancreatic ductal adenocarcinoma.

Science translational medicine

Zhou L,Husted H,Moore T,Lu M,Deng D,Liu Y,Ramachandran V,Arumugam T,Niehrs C,Wang H,Chiao P,Ling J,Curran MA,Maitra A,Hung MC,Lee JE,Logsdon CD,Hwang RF

Wed Oct 24 00:00:00 UTC 2018

MicroRNA-183 promotes the proliferation and metastasis of renal cell carcinoma through targeting Dickkopf-related protein 3.

Oncology letters

Zhang XL,Xu G,Zhou Y,Yan JJ

Sun Apr 01 00:00:00 UTC 2018

MicroRNA-183 promotes the proliferation and metastasis of renal cell carcinoma through targeting Dickkopf-related protein 3.

Oncology letters

Zhang XL,Xu G,Zhou Y,Yan JJ

Sun Apr 01 00:00:00 UTC 2018

Properties of the feline tumour suppressor reduced expression in immortalized cells (REIC/Dkk-3).

Veterinary and comparative oncology

Ochiai K,Oda H,Shono S,Kato Y,Sugihara S,Nakazawa S,Azakami D,Michishita M,Onozawa E,Bonkobara M,Sako T,Shun-Ai L,Ueki H,Watanabe M,Omi T

Fri Dec 01 00:00:00 UTC 2017

Properties of the feline tumour suppressor reduced expression in immortalized cells (REIC/Dkk-3).

Veterinary and comparative oncology

Ochiai K,Oda H,Shono S,Kato Y,Sugihara S,Nakazawa S,Azakami D,Michishita M,Onozawa E,Bonkobara M,Sako T,Shun-Ai L,Ueki H,Watanabe M,Omi T

Fri Dec 01 00:00:00 UTC 2017

Dickkopf 3 (Dkk3) Improves Amyloid-β Pathology, Cognitive Dysfunction, and Cerebral Glucose Metabolism in a Transgenic Mouse Model of Alzheimer's Disease.

Journal of Alzheimer's disease : JAD

Zhang L,Sun C,Jin Y,Gao K,Shi X,Qiu W,Ma C,Zhang L

Mon Apr 09 00:00:00 UTC 2018

Canine REIC/Dkk-3 interacts with SGTA and restores androgen receptor signalling in androgen-independent prostate cancer cell lines.

BMC veterinary research

Kato Y,Ochiai K,Kawakami S,Nakao N,Azakami D,Bonkobara M,Michishita M,Morimatsu M,Watanabe M,Omi T

Fri Jun 09 00:00:00 UTC 2017

Canine REIC/Dkk-3 interacts with SGTA and restores androgen receptor signalling in androgen-independent prostate cancer cell lines.

BMC veterinary research

Kato Y,Ochiai K,Kawakami S,Nakao N,Azakami D,Bonkobara M,Michishita M,Morimatsu M,Watanabe M,Omi T

Fri Jun 09 00:00:00 UTC 2017

Canine REIC/Dkk-3 interacts with SGTA and restores androgen receptor signalling in androgen-independent prostate cancer cell lines.

BMC veterinary research

Kato Y,Ochiai K,Kawakami S,Nakao N,Azakami D,Bonkobara M,Michishita M,Morimatsu M,Watanabe M,Omi T

Fri Jun 09 00:00:00 UTC 2017

Canine REIC/Dkk-3 interacts with SGTA and restores androgen receptor signalling in androgen-independent prostate cancer cell lines.

BMC veterinary research

Kato Y,Ochiai K,Kawakami S,Nakao N,Azakami D,Bonkobara M,Michishita M,Morimatsu M,Watanabe M,Omi T

Fri Jun 09 00:00:00 UTC 2017

Phosphatase WIP1 regulates adult neurogenesis and WNT signaling during aging.

The Journal of clinical investigation

Zhu Y,Demidov ON,Goh AM,Virshup DM,Lane DP,Bulavin DV

Tue Jul 01 00:00:00 UTC 2014

Phosphatase WIP1 regulates adult neurogenesis and WNT signaling during aging.

The Journal of clinical investigation

Zhu Y,Demidov ON,Goh AM,Virshup DM,Lane DP,Bulavin DV

Tue Jul 01 00:00:00 UTC 2014

SOX2 promotes tumor metastasis by stimulating epithelial-to-mesenchymal transition via regulation of WNT/β-catenin signal network.

Cancer letters

Li X,Xu Y,Chen Y,Chen S,Jia X,Sun T,Liu Y,Li X,Xiang R,Li N

Mon Aug 19 00:00:00 UTC 2013

SOX2 promotes tumor metastasis by stimulating epithelial-to-mesenchymal transition via regulation of WNT/β-catenin signal network.

Cancer letters

Li X,Xu Y,Chen Y,Chen S,Jia X,Sun T,Liu Y,Li X,Xiang R,Li N

Mon Aug 19 00:00:00 UTC 2013

microRNA-183 is an oncogene targeting Dkk-3 and SMAD4 in prostate cancer.

British journal of cancer

Ueno K,Hirata H,Shahryari V,Deng G,Tanaka Y,Tabatabai ZL,Hinoda Y,Dahiya R

Tue Apr 30 00:00:00 UTC 2013

microRNA-183 is an oncogene targeting Dkk-3 and SMAD4 in prostate cancer.

British journal of cancer

Ueno K,Hirata H,Shahryari V,Deng G,Tanaka Y,Tabatabai ZL,Hinoda Y,Dahiya R

Tue Apr 30 00:00:00 UTC 2013

microRNA-183 is an oncogene targeting Dkk-3 and SMAD4 in prostate cancer.

British journal of cancer

Ueno K,Hirata H,Shahryari V,Deng G,Tanaka Y,Tabatabai ZL,Hinoda Y,Dahiya R

Tue Apr 30 00:00:00 UTC 2013

microRNA-183 is an oncogene targeting Dkk-3 and SMAD4 in prostate cancer.

British journal of cancer

Ueno K,Hirata H,Shahryari V,Deng G,Tanaka Y,Tabatabai ZL,Hinoda Y,Dahiya R

Tue Apr 30 00:00:00 UTC 2013

Molecular cloning and tumour suppressor function analysis of canine REIC/Dkk-3 in mammary gland tumours.

Veterinary journal (London, England : 1997)

Ochiai K,Watanabe M,Azakami D,Michishita M,Yoshikawa Y,Udagawa C,Metheenukul P,Chahomchuen T,Aoki H,Kumon H,Morimatsu M,Omi T

Sun Sep 01 00:00:00 UTC 2013

Molecular cloning and tumour suppressor function analysis of canine REIC/Dkk-3 in mammary gland tumours.

Veterinary journal (London, England : 1997)

Ochiai K,Watanabe M,Azakami D,Michishita M,Yoshikawa Y,Udagawa C,Metheenukul P,Chahomchuen T,Aoki H,Kumon H,Morimatsu M,Omi T

Sun Sep 01 00:00:00 UTC 2013

Molecular cloning and tumour suppressor function analysis of canine REIC/Dkk-3 in mammary gland tumours.

Veterinary journal (London, England : 1997)

Ochiai K,Watanabe M,Azakami D,Michishita M,Yoshikawa Y,Udagawa C,Metheenukul P,Chahomchuen T,Aoki H,Kumon H,Morimatsu M,Omi T

Sun Sep 01 00:00:00 UTC 2013

Wnt antagonist DICKKOPF-3 (Dkk-3) induces apoptosis in human renal cell carcinoma.

Molecular carcinogenesis

Ueno K,Hirata H,Majid S,Chen Y,Zaman MS,Tabatabai ZL,Hinoda Y,Dahiya R

Wed Jun 01 00:00:00 UTC 2011

Wnt antagonist DICKKOPF-3 (Dkk-3) induces apoptosis in human renal cell carcinoma.

Molecular carcinogenesis

Ueno K,Hirata H,Majid S,Chen Y,Zaman MS,Tabatabai ZL,Hinoda Y,Dahiya R

Wed Jun 01 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: Dickkopf; dickkopf 3; dickkopf 3 homolog; Dickkopf gene 3; dickkopf homolog 3; Dickkopf-3; Dickkopf-related protein 3; Dickkopf3; DKK 3; dkk-3; hDkk 3; hDkk-3; hhDkk3; mDkk-3; regulated in glioma; RIG-like 5-6; RIG-like 7-1; Xenopus

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Gene Aliases: AW061014; C87148; DKK3; REIC; RIG; UNQ258/PRO295

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UniProt ID: (Human) Q9UBP4, (Mouse) Q9QUN9

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Entrez Gene ID: (Human) 27122, (Mouse) 50781

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Process(es)

anatomical structure morphogenesis Wnt signaling pathway regulation of transforming growth factor beta receptor signaling pathway adrenal gland development negative regulation of aldosterone biosynthetic process negative regulation of transcription, DNA-templated negative regulation of canonical Wnt signaling pathway negative regulation of anti-Mullerian hormone signaling pathway negative regulation of cortisol biosynthetic process multicellular organism development negative regulation of Wnt signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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DKK3 Polyclonal Antibody

DKK3 Antibody (10365-1-AP) in ICC/IF

Product Details

10365-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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