Proteintech

DLK1 Polyclonal Antibody

DLK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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DLK1 Antibody (10636-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human breast cancer using 10636-1-AP (DLK1 antibody) at dilution of 1:50 (under 40x lens).

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Product Details

10636-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Flow Cytometry (Flow)

Assay-dependent

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

DLK1 Fusion Protein Ag0991 (83-383 aa encoded by BC007741)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.34 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: ELCDRDVRA CSSAPCANNG TCVSLDDGLY ECSCAPGYSG KDCQKKDGPC VINGSPCQHG GTCVDDEGRA SHASCLCPPG FSGNFCEIVA NSCTPNPCEN DGVCTDIGGD FRCRCPAGFI DKTCSRPVTN CASSPCQNGG TCLQHTQVSY ECLCKPEFTG LTCVKKRALS PQQVTRLPNG YGLAYRLTPG VHELPVQQPE HRILKVSMKE LNKKTPLLTE GQAICFTILG VLTSLVVLGT VGIVFLNKCE TWVSNLRYNH MLRKKKNLLL QYNSGEDLAV NIIFPEKIDM TTFSKEAGDE EI (83-383 aa encoded by BC007741)

Target Information

The Delta-like 1 homolog (DLK1) is a transmembrane protein containing six epidermal growth factor repeats. It is involved in the differentiation of several cell types, including adipocytes and is also thought to be a tumor suppressor. The DLK1 gene is one of several imprinted genes located in a region on chromosome 14q32; certain mutations in this imprinted region can cause phenotypes similar to maternal and paternal uniparental disomy of chromosome 14 (UPD14). DLK1 is expressed from the paternal allele; a polymorphism within this gene has been associated with child and adolescent obesity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Identifying a dynamic transcriptomic landscape of the cynomolgus macaque placenta during pregnancy at single-cell resolution.

Developmental cell

Jiang X,Zhai J,Xiao Z,Wu X,Zhang D,Wan H,Xu Y,Qi L,Wang M,Yu D,Liu Y,Wu H,Sun R,Xia S,Yu K,Guo J,Wang H

Mon May 08 00:00:00 UTC 2023

FASEB bioAdvances

Sun X,Kato H,Sato H,Han X,Hirofuji Y,Kato TA,Sakai Y,Ohga S,Fukumoto S,Masuda K

Fri Jul 01 00:00:00 UTC 2022

LAMC1-mediated preadipocytes differentiation promoted peritoneum pre-metastatic niche formation and gastric cancer metastasis.

International journal of biological sciences

Fang Y,Dou R,Huang S,Han L,Fu H,Yang C,Song J,Zheng J,Zhang X,Liu K,Xiang Z,Zhang X,Wang S,Xiong B

Thu May 12 00:00:00 UTC 2022

Vitamin C attenuates predisposition to high-fat diet-induced metabolic dysregulation in GLUT10-deficient mouse model.

Genes & nutrition

Jiang CL,Tsao CY,Lee YC

Sat Jul 16 00:00:00 UTC 2022

A Na⁺/K⁺ ATPase Pump Regulates Chondrocyte Differentiation and Bone Length Variation in Mice.

Frontiers in cell and developmental biology

Marchini M,Ashkin MR,Bellini M,Sun MM,Workentine ML,Okuyan HM,Krawetz R,Beier F,Rolian C

Sat Jan 01 00:00:00 UTC 2022

Visceral adipogenesis inhibited by Pref-1 is associated with peritoneal angiogenesis.

Nephrology (Carlton, Vic.)

Jing R,Feng H,Jiang N,Zhang H,Fang W,Ni Z,Yuan J

Sun Mar 01 00:00:00 UTC 2020

TEA Domain Transcription Factor 4 Is the Major Mediator of Yes-Associated Protein Oncogenic Activity in Mouse and Human Hepatoblastoma.

The American journal of pathology

Zhang J,Liu P,Tao J,Wang P,Zhang Y,Song X,Che L,Sumazin P,Ribback S,Kiss A,Schaff Z,Cigliano A,Dombrowski F,Cossu C,Pascale RM,Calvisi DF,Monga SP,Chen X

Wed May 01 00:00:00 UTC 2019

Abnormally localized DLK1 interacts with NCOR1 in non-small cell lung cancer cell nuclear.

Bioscience reports

Tan J,Zhang S,Li L,Mu J,Wang Z,Zhang L,Jiang M,Li W,Yang X,Liu Y,Gao Y

Fri Dec 20 00:00:00 UTC 2019

Abnormally localized DLK1 interacts with NCOR1 in non-small cell lung cancer cell nuclear.

Bioscience reports

Tan J,Zhang S,Li L,Mu J,Wang Z,Zhang L,Jiang M,Li W,Yang X,Liu Y,Gao Y

Fri Dec 20 00:00:00 UTC 2019

GREB1 induced by Wnt signaling promotes development of hepatoblastoma by suppressing TGFβ signaling.

Nature communications

Matsumoto S,Yamamichi T,Shinzawa K,Kasahara Y,Nojima S,Kodama T,Obika S,Takehara T,Morii E,Okuyama H,Kikuchi A

Wed Aug 28 00:00:00 UTC 2019

The miR-181d-regulated metalloproteinase Adamts1 enzymatically impairs adipogenesis via ECM remodeling.

Cell death and differentiation

Chen SZ,Ning LF,Xu X,Jiang WY,Xing C,Jia WP,Chen XL,Tang QQ,Huang HY

Tue Nov 01 00:00:00 UTC 2016

Dietary vitamin A restriction affects adipocyte differentiation and fatty acid composition of intramuscular fat in Iberian pigs.

Meat science

Ayuso M,Óvilo C,Rodríguez-Bertos A,Rey AI,Daza A,Fenández A,González-Bulnes A,López-Bote CJ,Isabel B

Thu Oct 01 00:00:00 UTC 2015

Dietary vitamin A restriction affects adipocyte differentiation and fatty acid composition of intramuscular fat in Iberian pigs.

Meat science

Ayuso M,Óvilo C,Rodríguez-Bertos A,Rey AI,Daza A,Fenández A,González-Bulnes A,López-Bote CJ,Isabel B

Thu Oct 01 00:00:00 UTC 2015

Activation of β-catenin and Yap1 in human hepatoblastoma and induction of hepatocarcinogenesis in mice.

Gastroenterology

Tao J,Calvisi DF,Ranganathan S,Cigliano A,Zhou L,Singh S,Jiang L,Fan B,Terracciano L,Armeanu-Ebinger S,Ribback S,Dombrowski F,Evert M,Chen X,Monga SPS

Mon Sep 01 00:00:00 UTC 2014

Activation of β-catenin and Yap1 in human hepatoblastoma and induction of hepatocarcinogenesis in mice.

Gastroenterology

Tao J,Calvisi DF,Ranganathan S,Cigliano A,Zhou L,Singh S,Jiang L,Fan B,Terracciano L,Armeanu-Ebinger S,Ribback S,Dombrowski F,Evert M,Chen X,Monga SPS

Mon Sep 01 00:00:00 UTC 2014

DLK1 promotes lung cancer cell invasion through upregulation of MMP9 expression depending on Notch signaling.

PloS one

Li L,Tan J,Zhang Y,Han N,Di X,Xiao T,Cheng S,Gao Y,Liu Y

Fri May 29 00:00:00 UTC 2015

DLK1 promotes lung cancer cell invasion through upregulation of MMP9 expression depending on Notch signaling.

PloS one

Li L,Tan J,Zhang Y,Han N,Di X,Xiao T,Cheng S,Gao Y,Liu Y

Fri May 29 00:00:00 UTC 2015

Delta-like 1 homologue is a hypothalamus-enriched protein that is present in orexin-containing neurones of the lateral hypothalamic area.

Journal of neuroendocrinology

Meister B,Perez-Manso M,Daraio T

Mon Jul 01 00:00:00 UTC 2013

Delta-like 1 homologue is a hypothalamus-enriched protein that is present in orexin-containing neurones of the lateral hypothalamic area.

Journal of neuroendocrinology

Meister B,Perez-Manso M,Daraio T

Mon Jul 01 00:00:00 UTC 2013

Recovery of mature hepatocytic phenotype following bile ductular transdifferentiation of rat hepatocytes in vitro.

The American journal of pathology

Sone M,Nishikawa Y,Nagahama Y,Kumagai E,Doi Y,Omori Y,Yoshioka T,Tokairin T,Yoshida M,Sugiyama T,Enomoto K

Sat Dec 01 00:00:00 UTC 2012

Recovery of mature hepatocytic phenotype following bile ductular transdifferentiation of rat hepatocytes in vitro.

The American journal of pathology

Sone M,Nishikawa Y,Nagahama Y,Kumagai E,Doi Y,Omori Y,Yoshioka T,Tokairin T,Yoshida M,Sugiyama T,Enomoto K

Sat Dec 01 00:00:00 UTC 2012

Paternal uniparental disomy 14 and related disorders: placental gene expression analyses and histological examinations.

Epigenetics

Kagami M,Matsuoka K,Nagai T,Yamanaka M,Kurosawa K,Suzumori N,Sekita Y,Miyado M,Matsubara K,Fuke T,Kato F,Fukami M,Ogata T

Mon Oct 01 00:00:00 UTC 2012

Paternal uniparental disomy 14 and related disorders: placental gene expression analyses and histological examinations.

Epigenetics

Kagami M,Matsuoka K,Nagai T,Yamanaka M,Kurosawa K,Suzumori N,Sekita Y,Miyado M,Matsubara K,Fuke T,Kato F,Fukami M,Ogata T

Mon Oct 01 00:00:00 UTC 2012

Gene dosage effects of the imprinted delta-like homologue 1 (dlk1/pref1) in development: implications for the evolution of imprinting.

PLoS genetics

da Rocha ST,Charalambous M,Lin SP,Gutteridge I,Ito Y,Gray D,Dean W,Ferguson-Smith AC

Sun Feb 01 00:00:00 UTC 2009

Gene dosage effects of the imprinted delta-like homologue 1 (dlk1/pref1) in development: implications for the evolution of imprinting.

PLoS genetics

da Rocha ST,Charalambous M,Lin SP,Gutteridge I,Ito Y,Gray D,Dean W,Ferguson-Smith AC

Sun Feb 01 00:00:00 UTC 2009

Macrophage-secreted factors promote a profibrotic phenotype in human preadipocytes.

Molecular endocrinology (Baltimore, Md.)

Keophiphath M,Achard V,Henegar C,Rouault C,Clément K,Lacasa D

Thu Jan 01 00:00:00 UTC 2009

Macrophage-secreted factors promote a profibrotic phenotype in human preadipocytes.

Molecular endocrinology (Baltimore, Md.)

Keophiphath M,Achard V,Henegar C,Rouault C,Clément K,Lacasa D

Thu Jan 01 00:00:00 UTC 2009

CLA differently regulates adipogenesis in stromal vascular cells from porcine subcutaneous adipose and skeletal muscle.

Journal of lipid research

Zhou X,Li D,Yin J,Ni J,Dong B,Zhang J,Du M

Wed Aug 01 00:00:00 UTC 2007

CLA differently regulates adipogenesis in stromal vascular cells from porcine subcutaneous adipose and skeletal muscle.

Journal of lipid research

Zhou X,Li D,Yin J,Ni J,Dong B,Zhang J,Du M

Wed Aug 01 00:00:00 UTC 2007

Bioinformatics

Protein Aliases: Adipocyte differentiation inhibitor protein; delta-like 1 homolog; DLK-1; fetal antigen 1; pG2; Preadipocyte factor 1; preadipocyte factor-1; PREF; Pref-1; Protein delta homolog 1; secredeltin; stromal cell derived protein 1

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Gene Aliases: AW742678; Delta1; DLK; DLK-1; DLK1; DlkI; FA1; Ly107; Peg9; pG2; Pref-1; PREF1; Scp-1; SCP1; ZOG

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UniProt ID: (Human) P80370, (Mouse) Q09163, (Rat) O70534

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Entrez Gene ID: (Human) 8788, (Mouse) 13386, (Rat) 114587

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Function(s)

molecular_function calcium ion binding protein binding

Process(es)

embryo development post-embryonic development regulation of gene expression cell differentiation negative regulation of Notch signaling pathway embryonic skeletal system development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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DLK1 Polyclonal Antibody

DLK1 Antibody (10636-1-AP) in IHC (P)

Product Details

10636-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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