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Invitrogen
DLL1 (delta-like 1) Monoclonal Antibody (HMD1-5), Functional Grade, eBioscience™
Product Details
16-5767-85
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Armenian hamster
/ IgG
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
HMD1-5
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2016576
Product Specific Information
Description: The HMD1-5 monoclonal antibody reacts with mouse Delta-like 1, one of five type I transmembrane proteins that serves as a Notch receptor ligand. Upon binding the Notch receptor (e.g. Notch 1-4), Delta-like 1 undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by gamma-secretase. The second cleavage event releases an intracellular fragment whose biological function remains controversial. Delta-like 1 is expressed by thymic stromal cells, as well as on macrophages, dendritic cells, and stromal cells in the spleen. This protein is also expressed in nonhematopoietic tissues such as the lung and brain. Delta-like 1 has been linked to B cell development and differentiation, especially marginal zone and plasma cells, regulation of splenic dendritic cells, and leukemogenesis.
This monoclonal antibody has been reported to have neutralizing activity.
Applications Reported: This HMD1-5 antibody has been reported for use in functional assays.
Applications Tested: This HMD1-5 antibody has been tested by flow cytometric analysis of Delta-like 1-transfected CHO cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
Delta-like protein 1 (DLL1) is a transmembrane protein characterized by epidermal growth factor (EGF)-like repeats. It is a ligand for Notch receptor. It blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: delta like-1; Delta-like protein 1; Delta1; Drosophila Delta homolog 1; OTTHUMP00000017690
Gene Aliases: Delta1; Dll1
UniProt ID: (Mouse) Q61483
Entrez Gene ID: (Mouse) 13388
in utero embryonic development
somitogenesis
somite specification
heart looping
cell communication
Notch signaling pathway
cell-cell signaling
multicellular organism development
determination of left/right symmetry
compartment pattern specification
nervous system development
organ morphogenesis
auditory receptor cell fate commitment
cell differentiation
negative regulation of interleukin-10 production
inner ear morphogenesis
odontogenesis of dentin-containing tooth
auditory receptor cell differentiation
negative regulation of cell differentiation
negative regulation of auditory receptor cell differentiation
negative regulation of myeloid cell differentiation
negative regulation of neuron differentiation
positive regulation of Notch signaling pathway
positive regulation of transcription from RNA polymerase II promoter
inner ear development
regulation of neurogenesis
Notch signaling involved in heart development
left/right axis specification
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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