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DLL4 (soluble) Antibody (500-P279-1MG) in WB
Western Blot: To detect Human sDLL-4 by Western Blot analysis DLL4 (soluble) Polyclonal Antibody (Product # 500-P279-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human sDLL-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P279-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
HEK293 cells-derived Recombinant Human sDLL-4
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930219
Product Specific Information
AA Sequence of recombinant protein: SGVFQLQLQE FINERGVLAS GRPCEPGCRT FFRVCLKHFQ AVVSPGPCTF GTVSTPVLGT NSFAVRDDSS GGGRNPLQLP FNFTWPGTFS LIIEAWHAPG DDLRPEALPP DALISKIAIQ GSLAVGQNWL LDEQTSTLTR LRYSYRVICS DNYYGDNCSR LCKKRNDHFG HYVCQPDGNL SCLPGWTGEY CQQPICLSGC HEQNGYCSKP AECLCRPGWQ GRLCNECIPH NGCRHGTCST PWQCTCDEGW GGLFCDQDLN YCTHHSPCKN GATCSNSGQR SYTCTCRPGY TGVDCELELS ECDSNPCRNG GSCKDQEDGY HCLCPPGYYG LHCEHSTLSC ADSPCFNGGS CRERNQGANY ACECPPNFTG SNCEKKVDRC TSNPCANGGQ CLNRGPSRMC RCRPGFTGTY CELHVSDCAR NPCAHGGTCH DLENGLMCTC PAGFSGRRCE VRTSIDACAS SPCFNRATCY TDLSTDTFVC NCPYGFVGSR CEFPVGLP.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human sDLL-4. Anti-Human sDLL-4-specific antibody was purified by affinity chromatography employing an immobilized Human sDLL-4 matrix.
Sandwich ELISA: To detect Human sDLL-4 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human sDLL-4 (500-P279Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human sDLL-4.
Western Blot: To detect Human sDLL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human sDLL-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Delta-like ligand 4 (DLL4), also know as Delta-4, is a homologue of the Drosophila delta protein, and functions as a transmembrane bound ligand to the notch receptor Notch1. DLL4 is expressed in the vasculature and plays a critical role in vascular development. It is induced by vascular endothelial growth factor (VEGF), as a negative feedback regulator to regulate angiogenic sprouting and promote the formulation of a differentiated vascular network. DLL4 has been found to be strongly expressed in tumor vessels of clear-cell renal tumors and bladder cancer, and inhibition of DLL4 results in increased vascular proliferation but defective maturation. This in turn leads to a decrease in tumor growth, with no apparent toxicity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: delta 4; delta ligand 4; delta-like 4 homolog; delta-like 4 protein; Delta-like protein 4; Delta4; Drosophila Delta homolog 4; MGC126344; notch ligand delta-2; notch ligand DLL4
Gene Aliases: AOS6; DLL4; hdelta2; UNQ1895/PRO4341
UniProt ID: (Human) Q9NR61
Entrez Gene ID: (Human) 54567
negative regulation of transcription from RNA polymerase II promoter
angiogenesis
patterning of blood vessels
blood vessel remodeling
cardiac ventricle morphogenesis
cardiac atrium morphogenesis
ventricular trabecula myocardium morphogenesis
pericardium morphogenesis
signal transduction
Notch signaling pathway
Notch receptor processing
visual perception
blood circulation
negative regulation of cell proliferation
negative regulation of endothelial cell migration
positive regulation of gene expression
negative regulation of gene expression
T cell differentiation
dorsal aorta morphogenesis
cellular response to vascular endothelial growth factor stimulus
cellular response to fibroblast growth factor stimulus
negative regulation of Notch signaling pathway
positive regulation of Notch signaling pathway
regulation of neurogenesis
ventral spinal cord interneuron fate commitment
regulation of neural retina development
Notch signaling involved in heart development
blood vessel lumenization
negative regulation of cell migration involved in sprouting angiogenesis
negative regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis
positive regulation of neural precursor cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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