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DMPK Antibody (H00001760-M01) in WB
Western blot analysis of DMPK over-expressed 293 cell line, cotransfected with DMPK Validated Chimera RNAi (Cat # H00001760-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with DMPK monoclonal antibody (M01) clone 2F7 (Cat # H00001760-M01). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Product Details
H00001760-M01
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
2F7
Immunogen
DMPK (AAH62553, 303 a.a. approximately 420 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Conjugate
Form
Liquid
Concentration
See Label
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Sequence of this protein is as follows: DEGVPEEARD FIQRLLCPPE TRLGRGGAGD FRTHPFFFGL DWDGLRDSVP PFTPDFEGAT DTCNFDLVED GLTAMVSGGG ETLSDIREGA PLGVHLPFVG YSYSCMALRD SEVPGPTP
Target Information
DMPK is a serine/threonine kinase expressed in various muscle and nerve tissues. Mutations in the untranslated region of the DMPK gene are a characteristic of myotonic dystrophy. The protein encoded by this gene is a serine-threonine kinase that is closely related to other kinases that interact with members of the Rho family of small GTPases. Substrates for this enzyme include myogenin, the beta-subunit of the L-type calcium channels, and phospholemman. The 3' untranslated region of this gene contains 5-37 copies of a CTG trinucleotide repeat. Expansion of this unstable motif to 50-5,000 copies causes myotonic dystrophy type I, which increases in severity with increasing repeat element copy number. Repeat expansion is associated with condensation of local chromatin structure that disrupts the expression of genes in this region.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DM protein kinase; DM-kinase; DM1 protein kinase; DMK; DMPK; dystrophia myotonica-protein kinase; MT-PK; myotonic dystrophy associated protein kinase; Myotonic dystrophy protein kinase; myotonin protein kinase A; Myotonin-protein kinase; thymopoietin homolog
Gene Aliases: DM; DM1; DM1PK; DMK; DMPK; MDPK; MT-PK
UniProt ID: (Human) Q09013
Entrez Gene ID: (Human) 1760
regulation of sodium ion transport
protein phosphorylation
cellular calcium ion homeostasis
nuclear envelope organization
regulation of heart contraction
muscle cell apoptotic process
regulation of myotube differentiation
regulation of skeletal muscle contraction by calcium ion signaling
regulation of excitatory postsynaptic membrane potential involved in skeletal muscle contraction
peptidyl-serine phosphorylation
regulation of catalytic activity
regulation of synapse structural plasticity
regulation of cardiac conduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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