Proteintech

DNA Ligase I Polyclonal Antibody

DNA Ligase I Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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DNA Ligase I Antibody (18051-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human placenta using 18051-1-AP (DNA Ligase I antibody) at dilution of 1:100 (under 40x lens).

DNA Ligase I Antibody in Western Blot (WB)

DNA Ligase I Antibody in Immunoprecipitation (IP)

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Product Details

18051-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

DNA Ligase I Fusion Protein Ag12508 (670-919 aa encoded by BC108318)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.14 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: LVREPLSRR RQLLRENFVE TEGEFVFATS LDTKDIEQIA EFLEQSVKDS CEGLMVKTLD VDATYEIAKR SHNWLKLKKD YLDGVGDTLD LVVIGAYLGR GKRAGRYGGF LLASYDEDSE ELQAICKLGT GFSDEELEEH HQSLKALVLP SPRPYVRIDG AVIPDHWLDP SAVWEVKCAD LSLSPIYPAA RGLVDSDKGI SLRFPRFIRV REDKQPEQAT TSAQVACLYR KQSQIQNQQG EDSGSDPEDT Y (670-919 aa encoded by BC108318)

Target Information

The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. There are three alternatively spliced transcript variants encoding the same protein described for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cas9-induced large deletions and small indels are controlled in a convergent fashion.

Nature communications

Kosicki M,Allen F,Steward F,Tomberg K,Pan Y,Bradley A

Tue Jun 14 00:00:00 UTC 2022

eccDNAs are apoptotic products with high innate immunostimulatory activity.

Nature

Wang Y,Wang M,Djekidel MN,Chen H,Liu D,Alt FW,Zhang Y

Mon Nov 01 00:00:00 UTC 2021

The histone and non-histone methyllysine reader activities of the UHRF1 tandem Tudor domain are dispensable for the propagation of aberrant DNA methylation patterning in cancer cells.

Epigenetics & chromatin

Vaughan RM,Kupai A,Foley CA,Sagum CA,Tibben BM,Eden HE,Tiedemann RL,Berryhill CA,Patel V,Shaw KM,Krajewski K,Strahl BD,Bedford MT,Frye SV,Dickson BM,Rothbart SB

Fri Oct 23 00:00:00 UTC 2020

Biallelic mutations in DNA ligase 1 underlie a spectrum of immune deficiencies.

The Journal of clinical investigation

Maffucci P,Chavez J,Jurkiw TJ,O'Brien PJ,Abbott JK,Reynolds PR,Worth A,Notarangelo LD,Felgentreff K,Cortes P,Boisson B,Radigan L,Cobat A,Dinakar C,Ehlayel M,Ben-Omran T,Gelfand EW,Casanova JL,Cunningham-Rundles C

Mon Dec 03 00:00:00 UTC 2018

Biallelic mutations in DNA ligase 1 underlie a spectrum of immune deficiencies.

The Journal of clinical investigation

Mon Dec 03 00:00:00 UTC 2018

Nuclear localization of Beclin 1 promotes radiation-induced DNA damage repair independent of autophagy.

Scientific reports

Xu F,Fang Y,Yan L,Xu L,Zhang S,Cao Y,Xu L,Zhang X,Xie J,Jiang G,Ge C,An N,Zhou D,Yuan N,Wang J

Mon Mar 27 00:00:00 UTC 2017

Nuclear localization of Beclin 1 promotes radiation-induced DNA damage repair independent of autophagy.

Scientific reports

Xu F,Fang Y,Yan L,Xu L,Zhang S,Cao Y,Xu L,Zhang X,Xie J,Jiang G,Ge C,An N,Zhou D,Yuan N,Wang J

Mon Mar 27 00:00:00 UTC 2017

Redundant function of DNA ligase 1 and 3 in alternative end-joining during immunoglobulin class switch recombination.

Proceedings of the National Academy of Sciences of the United States of America

Masani S,Han L,Meek K,Yu K

Tue Feb 02 00:00:00 UTC 2016

Redundant function of DNA ligase 1 and 3 in alternative end-joining during immunoglobulin class switch recombination.

Proceedings of the National Academy of Sciences of the United States of America

Masani S,Han L,Meek K,Yu K

Tue Feb 02 00:00:00 UTC 2016

Ligase I and ligase III mediate the DNA double-strand break ligation in alternative end-joining.

Proceedings of the National Academy of Sciences of the United States of America

Lu G,Duan J,Shu S,Wang X,Gao L,Guo J,Zhang Y

Tue Feb 02 00:00:00 UTC 2016

Ligase I and ligase III mediate the DNA double-strand break ligation in alternative end-joining.

Proceedings of the National Academy of Sciences of the United States of America

Lu G,Duan J,Shu S,Wang X,Gao L,Guo J,Zhang Y

Tue Feb 02 00:00:00 UTC 2016

DNA ligase I is not essential for mammalian cell viability.

Cell reports

Han L,Masani S,Hsieh CL,Yu K

Thu Apr 24 00:00:00 UTC 2014

DNA ligase I is not essential for mammalian cell viability.

Cell reports

Han L,Masani S,Hsieh CL,Yu K

Thu Apr 24 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: DNA ligase (ATP) 1; DNA ligase 1; DNA ligase I; ligase 1; ligase I, DNA, ATP-dependent; MGC117397; MGC130025; Polydeoxyribonucleotide synthase [ATP] 1

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Gene Aliases: AL033288; Lig-1; LIG1; LigI

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UniProt ID: (Human) P18858, (Mouse) P37913

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Entrez Gene ID: (Human) 3978, (Mouse) 16881, (Rat) 81513

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Function(s)

DNA binding DNA ligase activity DNA ligase (ATP) activity ATP binding metal ion binding nucleotide binding ligase activity

Process(es)

telomere maintenance via recombination DNA metabolic process DNA repair transcription-coupled nucleotide-excision repair base-excision repair nucleotide-excision repair, DNA gap filling mismatch repair anatomical structure morphogenesis V(D)J recombination DNA ligation involved in DNA repair cell division DNA biosynthetic process Okazaki fragment processing involved in mitotic DNA replication DNA replication DNA ligation lagging strand elongation double-strand break repair via nonhomologous end joining DNA recombination cellular response to DNA damage stimulus cell cycle response to hydrogen peroxide removal of RNA primer involved in mitotic DNA replication nucleotide-excision repair

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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DNA Ligase I Polyclonal Antibody

DNA Ligase I Antibody (18051-1-AP) in IHC (P)

Product Details

18051-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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