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Invitrogen
DNA Ligase III Recombinant Rabbit Monoclonal Antibody (5I7T5)
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DNA Ligase III Antibody (MA5-51407) in IHC (P)
Immunohistochemistry analysis of DNA Ligase III in paraffin-embedded human liver cancer. Samples were incubated with DNA Ligase III Recombinant monoclonal antibody (Product # MA5-51407) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Product Details
MA5-51407
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
5I7T5
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 400-500 of human DNA Ligase III/LIG3 (NP_039269.2).
Conjugate
Form
Liquid
Concentration
0.09 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3093807
Product Specific Information
Immunogen sequence: IASRCTANDL KCIIRLIKHD LKMNSGAKHV LDALDPNAYE AFKASRNLQD VVERVLHNAQ EVEKEPGQRR ALSVQASLMT PVQPMLAEAC KSVEYAMKKC P
Target Information
There are four distinct ligase proteins found in mammalian cells. The activity of these DNA ligases is required for the joining of DNA strand breaks that occur during DNA replication, repair and recombination. DNA Ligase III gene product migrates as a 100 kDa (a-form) protein in somatic tissues, is ubiquitously expressed and complexes with XRCC-1 and a 97 kDa (beta form) protein that is found in testis. DNA Ligase III is involved in rejoining DNA single-strand breaks and in the base excision repair pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA ligase (ATP) 3; DNA ligase 3; DNA ligase III; ligase II, DNA, ATP-dependent; ligase III, DNA, ATP-dependent; Polydeoxyribonucleotide synthase [ATP] 3
Gene Aliases: D11Wsu78e; LIG2; LIG3
UniProt ID: (Human) P49916
Entrez Gene ID: (Human) 3980, (Rat) 303369, (Mouse) 16882
DNA ligation
lagging strand elongation
base-excision repair, DNA ligation
nucleotide-excision repair
double-strand break repair
double-strand break repair via nonhomologous end joining
DNA recombination
mitochondrion organization
V(D)J recombination
mitochondrial DNA repair
negative regulation of DNA recombination
DNA biosynthetic process
double-strand break repair via alternative nonhomologous end joining
DNA replication
DNA repair
cellular response to DNA damage stimulus
cell cycle
DNA ligation involved in DNA repair
cell division
negative regulation of mitochondrial DNA replication
double-strand break repair via homologous recombination
transcription-coupled nucleotide-excision repair
nucleotide-excision repair, DNA gap filling
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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