Invitrogen

DNA-PK Recombinant Rabbit Monoclonal Antibody (SC57-08)

2 Published Figures
3 References
View all (47) DNA-PK antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-32192

Applications
Tested Dilution
Publications

Western Blot (WB)

1:5000
View 2 publications 2 publications

Immunohistochemistry (IHC)

1:50-1:200
-

Immunohistochemistry (Paraffin) (IHC (P))

1:200
-

Immunocytochemistry (ICC/IF)

1:50-1:200
View 1 publication 1 publication

ChIP assay (ChIP)

2.5 µg/10^6 cells
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

SC57-08

Immunogen

Synthetic peptide within Human DNA PKcs aa 4062-4109
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2809479

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

DNA-PK is a heterotrimeric, DNA-dependent protein kinase that initiates signaling cascades in response to DNA damage. DNA-PK is an atypical kinase, closely related to the PI3 kinases, and is often potently inhibited by small molecules designed to target PI3 kinases. DNA-PK encodes a component of the mediator complex, a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. Multiple transcript variants encoding different isoforms have been found for DNA-PK.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNPK1; doxorubicin nephropathy; hyper-radiosensitivity of murine scid mutation, complementing 1; p460; S473K; Ser-473 kinase; severe combined immunodeficiency

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Gene Aliases: AI326420; AU019811; DNA-PKcs; DNAPDcs; DNAPK; DNPK1; DOXNPH; dxnph; HYRC; HYRC1; IMD26; p350; p460; PRKDC; scid; slip; XRCC7

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UniProt ID: (Human) P78527, (Mouse) P97313

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Entrez Gene ID: (Human) 5591, (Mouse) 19090, (Rat) 360748

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Function(s)
double-stranded DNA binding protein kinase activity protein serine/threonine kinase activity DNA-dependent protein kinase activity protein binding ATP binding transcription factor binding enzyme binding poly(A) RNA binding nucleotide binding DNA binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups phosphotransferase activity, alcohol group as acceptor molecular_function
Process(es)
telomere maintenance double-strand break repair via homologous recombination somitogenesis negative regulation of protein phosphorylation B cell lineage commitment pro-B cell differentiation T cell lineage commitment immunoglobulin production negative regulation of immunoglobulin production positive regulation of immune system process DNA repair double-strand break repair double-strand break repair via nonhomologous end joining protein phosphorylation cellular response to DNA damage stimulus brain development heart development intrinsic apoptotic signaling pathway in response to DNA damage response to ionizing radiation response to gamma radiation phosphorylation peptidyl-serine phosphorylation lymphocyte differentiation protein destabilization cellular response to insulin stimulus T cell differentiation in thymus V(D)J recombination immunoglobulin V(D)J recombination T cell receptor V(D)J recombination ectopic germ cell programmed cell death regulation of circadian rhythm positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of transcription from RNA polymerase II promoter positive regulation of fibroblast proliferation rhythmic process spleen development thymus development positive regulation of developmental growth signal transduction involved in mitotic G1 DNA damage checkpoint negative regulation of cellular senescence negative regulation of response to gamma radiation response to activity cellular protein modification process cell proliferation telomere capping positive regulation of type I interferon production regulation of smooth muscle cell proliferation double-strand break repair via alternative nonhomologous end joining
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