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DNA polymerase beta Polyclonal Antibody

DNA polymerase beta Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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DNA polymerase beta Antibody (18003-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human spleen using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 40x lens).

DNA polymerase beta Antibody in Western Blot (WB)

DNA polymerase beta Antibody in Immunoprecipitation (IP)

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Product Details

18003-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2400

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunoprecipitation (IP)

0.5-4.0 µg

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

DNA polymerase beta Fusion Protein Ag12496 (1-335 aa encoded by BC100288)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.33 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MSKRKAPQE TLNGGITDML TELANFEKNV SQAIHKYNAY RKAASVIAKY PHKIKSGAEA KKLPGVGTKI AEKIDEFLAT GKLRKLEKIR QDDTSSSINF LTRVSGIGPS AARKFVDEGI KTLEDLRKNE DKLNHHQRIG LKYFGDFEKR IPREEMLQMQ DIVLNEVKKV DSEYIATVCG SFRRGAESSG DMDVLLTHPS FTSESTKQPK LLHQVVEQLQ KVHFITDTLS KGETKFMGVC QLPSKNDEKE YPHRRIDIRL IPKDQYYCGV LYFTGSDIFN KNMRAHALEK GFTINEYTIR PLGVTGVAGE PLPVDSEKDI FDYIQWKYRE PKDRSE (1-335 aa encoded by B C100288)

Target Information

DNA polymerase beta comprises an amino-terminal 8- kDa domain and a carboxy-terminal 31- kDa domain. The N-terminal ssDNA binding domain has a deoxyribose phosphodiesterase activity while the C-terminal domain has a nucleotidyltransferase activity. Mammalian DNA polymerase beta, a DNA repair polymerase, is constitutively expressed in cultured cells, but treatment of cells with the DNA-alkylating agents such as N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) or methyl methanesulfonate up-regulates beta-pol level. DNA polymerase beta fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair.

Science advances

Nguyen DD,Kim E,Le NT,Ding X,Jaiswal RK,Kostlan RJ,Nguyen TNT,Shiva O,Le MT,Chai W

Wed May 10 00:00:00 UTC 2023

Single-molecule analysis of DNA-binding proteins from nuclear extracts (SMADNE).

Nucleic acids research

Schaich MA,Schnable BL,Kumar N,Roginskaya V,Jakielski RC,Urban R,Zhong Z,Kad NM,Van Houten B

Mon Apr 24 00:00:00 UTC 2023

Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia.

Nature communications

Lei H,Xu HZ,Shan HZ,Liu M,Lu Y,Fang ZX,Jin J,Jing B,Xiao XH,Gao SM,Gao FH,Xia L,Yang L,Liu LG,Wang WW,Liu CX,Tong Y,Wu YZ,Zheng JK,Chen GQ,Zhou L,Wu YL

Mon Jan 04 00:00:00 UTC 2021

Ligand-induced gene activation is associated with oxidative genome damage whose repair is required for transcription.

Proceedings of the National Academy of Sciences of the United States of America

Sengupta S,Wang H,Yang C,Szczesny B,Hegde ML,Mitra S

Tue Sep 08 00:00:00 UTC 2020

XRCC1 promotes replication restart, nascent fork degradation and mutagenic DNA repair in BRCA2-deficient cells.

NAR cancer

Eckelmann BJ,Bacolla A,Wang H,Ye Z,Guerrero EN,Jiang W,El-Zein R,Hegde ML,Tomkinson AE,Tainer JA,Mitra S

Tue Sep 01 00:00:00 UTC 2020

Acetylation of oxidized base repair-initiating NEIL1 DNA glycosylase required for chromatin-bound repair complex formation in the human genome increases cellular resistance to oxidative stress.

DNA repair

Sengupta S,Yang C,Hegde ML,Hegde PM,Mitra J,Pandey A,Dutta A,Datarwala AT,Bhakat KK,Mitra S

Tue Oct 02 00:00:00 UTC 2018

Overexpression of A613T and G462T variants of DNA polymerase β weakens chemotherapy sensitivity in esophageal cancer cell lines.

Cancer cell international

Wang Y,Chen X,Sun Q,Zang W,Li M,Dong Z,Zhao G

Wed Sep 30 00:00:00 UTC 2020

Overexpression of A613T and G462T variants of DNA polymerase β weakens chemotherapy sensitivity in esophageal cancer cell lines.

Cancer cell international

Wang Y,Chen X,Sun Q,Zang W,Li M,Dong Z,Zhao G

Wed Sep 30 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: 5'-deoxyribose-phosphate lyase; 5'-dRP lyase; AP lyase; DNA pol beta; DNA polymerase beta; DNA polymerase beta subunit; DNA-directed DNA polymerase beta; MGC125976; POL B; Pol beta; polymerase (DNA directed), beta; polymerase (DNA) beta; polymerase B; polymerase-beta

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Gene Aliases: A430088C08Rik; POLB

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UniProt ID: (Human) P06746, (Rat) P06766, (Mouse) Q8K409

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Entrez Gene ID: (Human) 5423, (Rat) 29240, (Mouse) 18970

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Function(s)

damaged DNA binding DNA-directed DNA polymerase activity DNA-(apurinic or apyrimidinic site) lyase activity protein binding microtubule binding lyase activity enzyme binding metal ion binding DNA binding transferase activity nucleotidyltransferase activity DNA polymerase activity

Process(es)

DNA-dependent DNA replication DNA repair base-excision repair base-excision repair, base-free sugar-phosphate removal base-excision repair, gap-filling base-excision repair, DNA ligation pyrimidine dimer repair nucleotide-excision repair, DNA gap filling inflammatory response cellular response to DNA damage stimulus salivary gland morphogenesis aging intrinsic apoptotic signaling pathway in response to DNA damage response to gamma radiation somatic hypermutation of immunoglobulin genes response to ethanol lymph node development spleen development homeostasis of number of cells neuron apoptotic process response to hyperoxia immunoglobulin heavy chain V-D-J recombination DNA biosynthetic process DNA replication apoptotic process somatic diversification of immunoglobulins

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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DNA polymerase beta Polyclonal Antibody

DNA polymerase beta Antibody (18003-1-AP) in IHC (P)

Product Details

18003-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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