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DNAJB12 Antibody (PA5-89947) in WB
Western blot analysis of DNAJB12 in extracts of various cell lines. Samples were incubated with DNAJB12 Polyclonal antibody (Product # PA5-89947) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-89947
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 250-409 of human DNAJB12 (NP_0600963)
Conjugate
Form
Liquid
Concentration
1.94 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805859
Product Specific Information
Immunogen sequence: NVHVYSNGRM RYTYQQRQDR RDNQGDGGLG VFVQLMPILI LILVSALSQL MVSSPPYSLS PRPSVGHIHR RVTDHLGVVY YVGDTFSEEY TGSSLKTVER NVEDDYIANL RNNCWKEKQQ KEGLLYRARY FGDTDMYHRA QKMGTPSCSR LSEVQASLHG; Positive Samples: 293T, HT-29, BxPC-3, Mouse kidney, Mouse liver, Rat spleen; Cellular Location: Membrane, Single-pass membrane protein
Target Information
DNAJB12 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain (Ohtsuka and Hata, 2000).
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp586B2023; DnaJ (Hsp40) homolog, subfamily B, member 12; DnaJ homolog 10; DnaJ homolog subfamily B member 12; mDj10
Gene Aliases: DJ10; DNAJB12; mDj10
UniProt ID: (Human) Q9NXW2, (Mouse) Q9QYI4
Entrez Gene ID: (Human) 54788, (Rat) 294513, (Mouse) 56709
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