Proteintech

DNAJB2 Polyclonal Antibody

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DNAJB2 Antibody (10838-1-AP) in WB

Various lysates were subjected to SDS PAGE followed by western blot with (Product # 10838-1-AP) DNAJB2 antibody at dilution of 1:4,000 incubated at room temperature for 1.5 hours.

DNAJB2 Antibody in Immunoprecipitation (IP)

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Product Details

10838-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

Assay-dependent

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

DNAJB2 Fusion Protein Ag1271 (1-324 aa encoded by BC011609)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.18 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody detects both isoforms of DNAJB2 with MW between 32-40 kDa.

Immunogen sequence: MASYYEILD VPRSASADDI KKAYRRKALQ WHPDKNPDNK EFAEKKFKEV AEAYEVLSDK HKREIYDRYG REGLTGTGTG PSRAEAGSGG PGFTFTFRSP EEVFREFFGS GDPFAELFDD LGPFSELQNR GSRHSGPFFT FSSSFPGHSD FSSSSFSFSP GAGAFRSVST STTFVQGRRI TTRRIMENGQ ERVEVEEDGQ LKSVTINGVP DDLALGLELS RREQQPSVTS RSGGTQVQQT PASCPLDSDL SEDEDLQLAM AYSLSEMEAA GKKPAGGREA QHRRQGRPKA QHQDPGLGGT QEGARGEATK RSPSPEEKAS RCLIL (1-324 aa encoded by BC011609)

Target Information

The DnaJ family is one of the largest of all the chaperone families and has evolved with diverse cellular localization and functions. The presence of the J domain defines a protein as a member of the DnaJ family. DnaJ heat shock induced proteins are from the bacterium Escherichia coli and are under the control of the htpR regulatory protein. The DnaJ proteins play a critical role in the HSP 70 chaperone machine by interacting with HSP 70 to stimulate ATP hydrolysis. The proteins contain cysteine rich regions that are composed of zinc fingers that form a peptide binding domain responsible for the chaperone function. DnaJ proteins are important mediators of proteolysis and are involved in the regulation of protein degradation, exocytosis and endocytosis. DnaJB2 (DnaJ homolog subfamily B member 2), also known as HSJ1 or HSPF3, is expressed almost exclusively in the brain, with the highest levels in the frontal cortex and hippocampus. Two isoforms are produced due to alternative splicing.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

European journal of neurology

Saveri P,Magri S,Maderna E,Balistreri F,Lombardi R,Ciano C,Moda F,Garavaglia B,Reale C,Lauria Pinter G,Taroni F,Pareyson D,Pisciotta C

Fri Jul 01 00:00:00 UTC 2022

European journal of neurology

Saveri P,Magri S,Maderna E,Balistreri F,Lombardi R,Ciano C,Moda F,Garavaglia B,Reale C,Lauria Pinter G,Taroni F,Pareyson D,Pisciotta C

Fri Jul 01 00:00:00 UTC 2022

Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin.

Cancers

Alalem M,Bhosale M,Ranjan A,Yamamoto S,Kaida A,Nishikawa S,Parrales A,Farooki S,Anant S,Padhye S,Iwakuma T

Mon Aug 29 00:00:00 UTC 2022

PolyQ-expanded proteins impair cellular proteostasis of ataxin-3 through sequestering the co-chaperone HSJ1 into aggregates.

Scientific reports

Yue HW,Hong JY,Zhang SX,Jiang LL,Hu HY

Fri Apr 09 00:00:00 UTC 2021

PolyQ-expanded proteins impair cellular proteostasis of ataxin-3 through sequestering the co-chaperone HSJ1 into aggregates.

Scientific reports

Yue HW,Hong JY,Zhang SX,Jiang LL,Hu HY

Fri Apr 09 00:00:00 UTC 2021

HSP72 is a mitochondrial stress sensor critical for Parkin action, oxidative metabolism, and insulin sensitivity in skeletal muscle.

Diabetes

Drew BG,Ribas V,Le JA,Henstridge DC,Phun J,Zhou Z,Soleymani T,Daraei P,Sitz D,Vergnes L,Wanagat J,Reue K,Febbraio MA,Hevener AL

Thu May 01 00:00:00 UTC 2014

HSP72 is a mitochondrial stress sensor critical for Parkin action, oxidative metabolism, and insulin sensitivity in skeletal muscle.

Diabetes

Drew BG,Ribas V,Le JA,Henstridge DC,Phun J,Zhou Z,Soleymani T,Daraei P,Sitz D,Vergnes L,Wanagat J,Reue K,Febbraio MA,Hevener AL

Thu May 01 00:00:00 UTC 2014

A rare recessive distal hereditary motor neuropathy with HSJ1 chaperone mutation.

Annals of neurology

Blumen SC,Astord S,Robin V,Vignaud L,Toumi N,Cieslik A,Achiron A,Carasso RL,Gurevich M,Braverman I,Blumen N,Munich A,Barkats M,Viollet L

Sun Apr 01 00:00:00 UTC 2012

A rare recessive distal hereditary motor neuropathy with HSJ1 chaperone mutation.

Annals of neurology

Blumen SC,Astord S,Robin V,Vignaud L,Toumi N,Cieslik A,Achiron A,Carasso RL,Gurevich M,Braverman I,Blumen N,Munich A,Barkats M,Viollet L

Sun Apr 01 00:00:00 UTC 2012

A rare recessive distal hereditary motor neuropathy with HSJ1 chaperone mutation.

Annals of neurology

Blumen SC,Astord S,Robin V,Vignaud L,Toumi N,Cieslik A,Achiron A,Carasso RL,Gurevich M,Braverman I,Blumen N,Munich A,Barkats M,Viollet L

Sun Apr 01 00:00:00 UTC 2012

A rare recessive distal hereditary motor neuropathy with HSJ1 chaperone mutation.

Annals of neurology

Blumen SC,Astord S,Robin V,Vignaud L,Toumi N,Cieslik A,Achiron A,Carasso RL,Gurevich M,Braverman I,Blumen N,Munich A,Barkats M,Viollet L

Sun Apr 01 00:00:00 UTC 2012

DNAJB2 expression in normal and diseased human and mouse skeletal muscle.

The American journal of pathology

Claeys KG,Sozanska M,Martin JJ,Lacene E,Vignaud L,Stockholm D,Laforêt P,Eymard B,Kichler A,Scherman D,Voit T,Israeli D

Tue Jun 01 00:00:00 UTC 2010

DNAJB2 expression in normal and diseased human and mouse skeletal muscle.

The American journal of pathology

Claeys KG,Sozanska M,Martin JJ,Lacene E,Vignaud L,Stockholm D,Laforêt P,Eymard B,Kichler A,Scherman D,Voit T,Israeli D

Tue Jun 01 00:00:00 UTC 2010

DNAJB2 expression in normal and diseased human and mouse skeletal muscle.

The American journal of pathology

Claeys KG,Sozanska M,Martin JJ,Lacene E,Vignaud L,Stockholm D,Laforêt P,Eymard B,Kichler A,Scherman D,Voit T,Israeli D

Tue Jun 01 00:00:00 UTC 2010

DNAJB2 expression in normal and diseased human and mouse skeletal muscle.

The American journal of pathology

Claeys KG,Sozanska M,Martin JJ,Lacene E,Vignaud L,Stockholm D,Laforêt P,Eymard B,Kichler A,Scherman D,Voit T,Israeli D

Tue Jun 01 00:00:00 UTC 2010

Bioinformatics

Protein Aliases: DnaJ (Hsp40) homolog, subfamily B, member 10; DnaJ (Hsp40) homolog, subfamily B, member 2; DnaJ homolog subfamily B member 10; DnaJ homolog subfamily B member 2; dnaJ protein homolog 1; Heat shock 40 kDa protein 3; Heat shock protein J1; heat shock protein, DNAJ-like 1; heat shock protein, neuronal DNAJ-like 1; HSJ-1; mDj8

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Gene Aliases: 2700059H22Rik; CMT2T; Dnajb10; DNAJB2; DSMA5; HSJ-1; HSJ1; HSPF3; mDj8

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UniProt ID: (Human) P25686, (Mouse) Q9QYI5

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Entrez Gene ID: (Human) 3300, (Mouse) 56812, (Rat) 689593

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Function(s)

unfolded protein binding chaperone binding Hsp70 protein binding polyubiquitin binding proteasome binding

Process(es)

negative regulation of cell proliferation negative regulation of cell growth ER-associated ubiquitin-dependent protein catabolic process positive regulation of protein ubiquitination positive regulation of proteasomal ubiquitin-dependent protein catabolic process protein refolding negative regulation of inclusion body assembly negative regulation of protein deubiquitination protein folding response to unfolded protein

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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DNAJB2 Polyclonal Antibody

DNAJB2 Antibody (10838-1-AP) in WB

Product Details

10838-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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