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DNAJC11 Antibody (PA5-144019) in ICC/IF
Immunocytochemistry analysis of DNAJC11 in Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in DNAJC11 Polyclonal antibody (Product # PA5-144019) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropria... View More
Product Details
PA5-144019
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human DNAJC11 recombinant protein (Position: N12-Q551).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3075233
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human SH-SY5Y whole cell, human A549 whole cell, rat liver tissue, rat skeletal muscle tissue, mouse liver tissue, mouse skeletal muscle tissue, human HepG2 whole cell. ICC/IF: Hela cell. Flow: PC-3 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Belongs to the DNAJC11 family. Three named isoforms produced by alternative splicing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DnaJ (Hsp40) homolog, subfamily C, member 11; DnaJ homolog subfamily C member 11; novel DnaJ domain-containing protein
Gene Aliases: dJ126A5.1; DNAJC11; E030019A03Rik
UniProt ID: (Human) Q9NVH1, (Mouse) Q5U458
Entrez Gene ID: (Human) 55735, (Mouse) 230935, (Rat) 362666
Performance Guarantee
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