Invitrogen

DNAJC3 Monoclonal Antibody (J.98.6)

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DNAJC3 Antibody (MA5-14820) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), Hep G2 (Lane 2), Raji (Lane 3), NIH/3T3 (Lane 4), MOLT-4 (Lane 5), COLO 205 (Lane 6), tissue extracts of Mouse Pancreas (Lane 7), Mouse Stomach (Lane 8) and Mouse Colon (Lane 9). The blot was probed with Anti-DNAJC3 Monoclonal Antibody (Product # MA5-14820, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 58 kD... View More

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Product Details

MA5-14820

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 3 publications 3 publications

Immunoprecipitation (IP)

1:50

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

J.98.6

Immunogen

Synthetic peptide corresponding to the sequence of human p58IPK

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10984142

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

This gene encodes a protein with multiple tetratricopeptide repeat (TPR) motifs as well as the highly conserved J domain found in DNAJ chaperone family members. It is a member of the tetratricopeptide repeat family of proteins and acts as an inhibitor of the interferon-induced, dsRNA-activated protein kinase (PKR).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

XBP1 variant 1 promotes mitosis of cancer cells involving upregulation of the polyglutamylase TTLL6.

Human molecular genetics

Zhong Y,Yan W,Ruan J,Fang M,Yu C,Du S,Rai G,Tao D,Henderson MJ,Fang S

MA5-14820 was used in Western Blotting to suggest that Xv1 is essential for cancer cell mitosis, which is mediated, at least in part, by increasing TTLL6 expression.

Wed Aug 17 00:00:00 UTC 2022

Inhibition of eIF2α Dephosphorylation Protects Hepatocytes from Apoptosis by Alleviating ER Stress in Acute Liver Injury.

BioMed research international

Tang YJ,Chen H,Yi Y,Chen GM,Yang FW,Li Y,Tian RD,Huang WG,Cheng QJ,He YH

MA5-14820 was used in Western Blotting to investigate the effects of eIF2α phosphorylation on hepatocyte apoptosis and the ER stress mechanisms in acute liver injury.

Mon Mar 22 00:00:00 UTC 2021

Inhibiting alpha subunit of eukaryotic initiation factor 2 dephosphorylation protects injured hepatocytes and reduces hepatocyte proliferation in acute liver injury.

Croatian medical journal

Chen G,Yang X,He Y,Tang Y,Tian R,Huang W,Chen H,Yang F,Li Y,Lin S

Published figure using DNAJC3 monoclonal antibody (Product # MA5-14820) in Western Blot

Tue Dec 31 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: DnaJ (Hsp40) homolog, subfamily C, member 3; DnaJ (Hsp40) homolog, subfamily C, member 3A; DnaJ (Hsp40) homolog, subfamily C, member 3B; DnaJ homolog subfamily C member 3; Endoplasmic reticulum DNA J domain-containing protein 6; ER-resident protein ERdj6; FLJ21288; Interferon-induced, double-stranded RNA-activated protein kinase inhibitor; Protein kinase inhibitor of 58 kDa; Protein kinase inhibitor p58; protein kinase, interferon inducible double stranded RNA dependent inhibitor; protein-kinase, interferon-inducible double stranded RNA dependent inhibitor

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Gene Aliases: AA408985; ACPHD; AU067833; DNAJC3; Dnajc3a; Dnajc3b; ERdj6; HP58; mp58; P58; P58IPK; PRKRI

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UniProt ID: (Human) Q13217, (Mouse) Q91YW3

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Entrez Gene ID: (Human) 5611, (Mouse) 100037258

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Function(s)

protein kinase inhibitor activity protein kinase binding chaperone binding misfolded protein binding protein binding

Process(es)

positive regulation of translation initiation in response to endoplasmic reticulum stress IRE1-mediated unfolded protein response negative regulation of apoptotic process proteolysis involved in cellular protein catabolic process defense response to virus cellular response to cold negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation negative regulation of cyclin-dependent protein kinase activity negative regulation of protein kinase activity response to unfolded protein endoplasmic reticulum unfolded protein response

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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DNAJC3 Monoclonal Antibody (J.98.6)

DNAJC3 Antibody (MA5-14820) in WB

Product Details

MA5-14820

Western Blot (WB)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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