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Invitrogen
DNAJC6 Polyclonal Antibody
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FIGURE: 1 / 8
DNAJC6 Antibody (PA5-143944) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of DNAJC6 in A549 cells using DNAJC6 Polyclonal Antibody (Product # PA5-143944). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL. DyLight 550 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter ... View More
Product Details
PA5-143944
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human DNAJC6 recombinant protein (Position: R32-Y913).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3075158
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human K562 whole cell, human SH-SY5Y whole cell, rat brain tissue, rat PC-12 whole cell, mouse brain tissue. IHC: rat brain tissue. ICC/IF: A549 cell .|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
DNAJC6 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus, a glycine/phenylalanine (G/F)-rich region, and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Auxilin; DnaJ (Hsp40) homolog, subfamily B, member 6; DnaJ (Hsp40) homolog, subfamily C, member 6; DnaJ homolog subfamily C member 6; putative tyrosine-protein phosphatase auxilin
Gene Aliases: 2810027M23Rik; DJC6; DNAJC6; KIAA0473; mKIAA0473; PARK19
UniProt ID: (Human) O75061, (Mouse) Q80TZ3
Entrez Gene ID: (Human) 9829, (Mouse) 72685, (Rat) 313409
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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