Invitrogen

DNMT1 Monoclonal Antibody (60B1220.1)

DNMT1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

DNMT1 Antibody (MA5-16169) in ICC/IF

Immunofluorescence analysis of DNMT1 was performed using 70% confluent log phase K-562 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with DNMT1 Monoclonal Antibody (60B1220.1) (Product # MA5-16169) at 1:10 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766... View More

DNMT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DNMT1 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

DNMT1 Antibody — Antibody specificity was demonstrated by detection of enrichment of the target protein at specific gene loci. Chromatin Immunoprecipitation (ChIP) was performed using Anti-DNMT1 monoclonal antibody (Product # MA5-16169) using PCR primer pairs over mir-137, POMC, IL1R2 (positive) and SAT2 satellite repeats (negative). {RE}

Product Details

MA5-16169

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.5 µg/mL

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunohistochemistry (Frozen) (IHC (F))

1:500

Immunohistochemistry - Free Floating (IHC (Free))

1:500

Immunocytochemistry (ICC/IF)

1:10

View 2 publications 2 publications

Flow Cytometry (Flow)

1 - 5 µg/mL

Immunoprecipitation (IP)

1:10-1:500

View 1 publication 1 publication

ChIP assay (ChIP)

1:20-1:1,000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Sheep, Pig, Rat

Published species

Gerbil, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

60B1220.1

Immunogen

Synthetic peptide corresponding to amino acids 637-650 (EKDDREDKENAFKR) of human Dnmt1.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2537688

Product Specific Information

Suggested positive control: human kidney (IHC), mouse ES cells (WB).

Target Information

This gene encodes a subunit of the augmin complex, which regulates centrosome and mitotic spindle integrity, and is necessary for the completion of cytokinesis. The encoded protein was identified by interaction with ubiquitin C-terminal hydrolase 37. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

DNMT1-Mediated DNA Methylation Targets CDKN2B to Promote the Repair of Retinal Ganglion Cells in Streptozotocin-Induced Mongolian Gerbils during Diabetic Retinopathy.

Computational and mathematical methods in medicine

Wang X,Zhang J,Liao Y,Jin Y,Yu X,Li H,Yang Q,Li X,Chen R,Wu D,Zhu H

MA5-16169 was used in Western Blot to explore the role of DNA cytosine-5-methyltransferase 1 (DNMT1) in the development of early diabetic retinopathy and its potential underlying mechanism.

Mon Mar 28 00:00:00 UTC 2022

DNMT1-Mediated DNA Methylation Targets CDKN2B to Promote the Repair of Retinal Ganglion Cells in Streptozotocin-Induced Mongolian Gerbils during Diabetic Retinopathy.

Computational and mathematical methods in medicine

Wang X,Zhang J,Liao Y,Jin Y,Yu X,Li H,Yang Q,Li X,Chen R,Wu D,Zhu H

MA5-16169 was used in Western Blot to explore the role of DNA cytosine-5-methyltransferase 1 (DNMT1) in the development of early diabetic retinopathy and its potential underlying mechanism.

Mon Mar 28 00:00:00 UTC 2022

LncRNA affects epigenetic reprogramming of porcine embryo development by regulating global epigenetic modification and the downstream gene SIN3A.

Frontiers in physiology

Zhang D,Zhou Y,Huang R,Zhai Y,Wu D,An X,Zhang S,Shi L,Li Q,Kong X,Yu H,Li Z

Published figure using DNMT1 monoclonal antibody (Product # MA5-16169) in Immunocytochemistry

Tue Oct 04 00:00:00 UTC 2022

Myocardial tissue-specific Dnmt1 knockout in rats protects against pathological injury induced by Adriamycin.

Laboratory investigation; a journal of technical methods and pathology

Wu TT,Ma YW,Zhang X,Dong W,Gao S,Wang JZ,Zhang LF,Lu D

MA5-16169 was used in Western Blot, Immunocytochemistry to investigate the impact of genome-wide cardiac DNA methyltransferase deficiency on the physiology and pathological processes of heart tissue under stress within an in vivo rat model.

Wed Jul 01 00:00:00 UTC 2020

Myocardial tissue-specific Dnmt1 knockout in rats protects against pathological injury induced by Adriamycin.

Laboratory investigation; a journal of technical methods and pathology

Wu TT,Ma YW,Zhang X,Dong W,Gao S,Wang JZ,Zhang LF,Lu D

Wed Jul 01 00:00:00 UTC 2020

DNMT1 and HDAC2 Cooperate to Facilitate Aberrant Promoter Methylation in Inorganic Phosphate-Induced Endothelial-Mesenchymal Transition.

PloS one

Tan X,Xu X,Zeisberg M,Zeisberg EM

MA5-16169 was used in western blot to study the interaction between DNMT1 and HDAC2 and how they cooperate to facilitate aberrant promoter methylation in inorganic phosphate-induced endothelial-mesenchymal transition

Tue Jul 26 00:00:00 UTC 2016

DNMT1 and HDAC2 Cooperate to Facilitate Aberrant Promoter Methylation in Inorganic Phosphate-Induced Endothelial-Mesenchymal Transition.

PloS one

Tan X,Xu X,Zeisberg M,Zeisberg EM

Tue Jul 26 00:00:00 UTC 2016

DNMT1 and HDAC2 Cooperate to Facilitate Aberrant Promoter Methylation in Inorganic Phosphate-Induced Endothelial-Mesenchymal Transition.

PloS one

Tan X,Xu X,Zeisberg M,Zeisberg EM

Tue Jul 26 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: CXXC-type zinc finger protein 9; DNA (cytosine 5 ) methyltransferase 1; DNA (cytosine-5)-methyltransferase 1; DNA methyltransferase (cytosine 5 ) methyltransferase; DNA methyltransferase (cytosine-5) 1; DNA methyltransferase 1; DNA methyltransferase b; DNA methyltransferase HsaI; DNA methyltransferase I; DNA methyltransferase MmuI; DNA MTase HsaI; DNA MTase MmuI; DNA MTase RnoIP; Dnmt1; FLJ16293; m.RnoIP; MCMT; MGC104992

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Gene Aliases: ADCADN; AIM; BOS_7601; CXXC9; DNMT; DNMT1; Dnmt1o; HSN1E; m.HsaI; m.MmuI; MCMT; Met-1; Met1; MommeD2; MTase; Uim

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UniProt ID: (Bovine) Q24K09, (Human) P26358, (Mouse) P13864

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Entrez Gene ID: (Bovine) 281119, (Sheep) 443549, (Pig) 606746, (Human) 1786, (Rat) 84350, (Mouse) 13433

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Function(s)

RNA binding DNA (cytosine-5-)-methyltransferase activity transcription factor binding zinc ion binding methyl-CpG binding DNA binding double-stranded DNA binding protein binding DNA-methyltransferase activity protein domain specific binding estrogen receptor binding histone deacetylase binding unmethylated CpG binding DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates promoter-specific chromatin binding chromatin binding methyltransferase activity S-adenosylmethionine-dependent methyltransferase activity catalytic activity transferase activity metal ion binding

Process(es)

transcription, DNA-templated maintenance of DNA methylation positive regulation of gene expression gene silencing chromatin modification regulation of cell proliferation negative regulation of transcription, DNA-templated positive regulation of histone H3-K4 methylation negative regulation of histone H3-K9 methylation cellular response to amino acid stimulus negative regulation of transcription from RNA polymerase II promoter DNA methylation regulation of transcription, DNA-templated brain development aging response to heat response to toxic substance response to organic substance response to ionizing radiation response to lead ion DNA methylation on cytosine within a CG sequence regulation of gene expression response to activity neuron differentiation response to caffeine response to nutrient levels response to estradiol response to lipopolysaccharide DNA methylation on cytosine response to vitamin A response to testosterone cellular response to platelet-derived growth factor stimulus response to antidepressant response to drug DNA hypermethylation response to ethanol S-adenosylhomocysteine metabolic process S-adenosylmethioninamine metabolic process S-adenosylmethionine metabolic process cellular response to lead ion cellular response to transforming growth factor beta stimulus C-5 methylation of cytosine cellular response to nerve growth factor stimulus Ras protein signal transduction covalent chromatin modification negative regulation of gene expression, epigenetic positive regulation of methylation-dependent chromatin silencing metabolic process methylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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DNMT1 Monoclonal Antibody (60B1220.1)

DNMT1 Antibody (MA5-16169) in ICC/IF

Product Details

MA5-16169

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunohistochemistry - Free Floating (IHC (Free))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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