Invitrogen

DNMT3A Polyclonal Antibody

2 Published Figures

3 References

View all (31) DNMT3A antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)
Published Figures (2)

DNMT3A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 4

DNMT3A Antibody (PA3-16557) in IHC (P)

Immunohistochemical analysis of DNMT3A in human testis. Samples were incubated with DNMT3A polyclonal antibody (Product # PA3-16557) followed by using DAB with hematoxylin counterstain.

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Product Details

PA3-16557

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:500

Immunoprecipitation (IP)

1:10-1:500

Product Specifications

Species Reactivity

Human, Mouse

Published species

Hamster, Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This antibody was raised against amino acids 10-118 of the human DNMT3a protein.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_568519

Product Specific Information

This antibody does not neutralize the enzymatic activity of its corresponding methyltransferase.

Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Suggested positive control: antigen standard for DNMT3A (transient overexpression lysate), HCT116 cell lysates.

Target Information

Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Downregulation of DNA methylation enhances differentiation of THP-1 cells and induces M1 polarization of differentiated macrophages.

Scientific reports

Park J,Luo Y,Park JW,Kim SH,Hong YJ,Lim Y,Seo YJ,Bae J,Seo SB

PA3-16557 was used in Western Blotting to suggest that UHRF1 and DNMT1 play a pivotal role in regulating differentiation and proliferation of THP-1 cells and targeting these proteins may improve the efficiency of differentiation therapy in AML patients.

Sat Aug 12 00:00:00 UTC 2023

Downregulation of DNA methylation enhances differentiation of THP-1 cells and induces M1 polarization of differentiated macrophages.

Scientific reports

Park J,Luo Y,Park JW,Kim SH,Hong YJ,Lim Y,Seo YJ,Bae J,Seo SB

Sat Aug 12 00:00:00 UTC 2023

Absence of genomic hypomethylation or regulation of cytosine-modifying enzymes with aging in male and female mice.

Epigenetics & chromatin

Hadad N,Masser DR,Logan S,Wronowski B,Mangold CA,Clark N,Otalora L,Unnikrishnan A,Ford MM,Giles CB,Wren JD,Richardson A,Sonntag WE,Stanford DR,Freeman W

Published figure using DNMT3A polyclonal antibody (Product # PA3-16557) in Western Blot

Thu Jul 14 00:00:00 UTC 2016

Cyclical DNA Methyltransferase 3a Expression Is a Seasonal and Estrus Timer in Reproductive Tissues.

Endocrinology

Lynch EW,Coyle CS,Lorgen M,Campbell EM,Bowman AS,Stevenson TJ

PA3-16557 was used in Immunohistochemistry (Frozen) to indicate that dnmt3a shows marked photoperiod and estrus plasticity that likely has broad downstream effects on the timing of the genomic control of reproductive function.

Wed Jun 01 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: AU015806; Cysteine methyltransferase DNMT3A; DNA (cytosine-5)-methyltransferase 3A; DNA (cytosine-5-)-methyltransferase 3 alpha; DNA cytosine methyltransferase 3A2; DNA methyltransferase HsaIIIA; DNA methyltransferase MmuIIIA; DNA MTase HsaIIIA; DNA MTase MmuIIIA; Dnmt3a; W91664

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Gene Aliases: DNMT3A; DNMT3A2; M.HsaIIIA; MmuIIIA; TBRS

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UniProt ID: (Human) Q9Y6K1, (Mouse) O88508

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Entrez Gene ID: (Human) 1788, (Mouse) 13435

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Function(s)

DNA binding chromatin binding DNA (cytosine-5-)-methyltransferase activity protein binding DNA-methyltransferase activity identical protein binding unmethylated CpG binding metal ion binding DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates methyltransferase activity transferase activity

Process(es)

negative regulation of transcription from RNA polymerase II promoter DNA methylation methylation-dependent chromatin silencing regulation of gene expression by genetic imprinting transcription, DNA-templated regulation of transcription, DNA-templated spermatogenesis maintenance of DNA methylation DNA methylation on cytosine within a CG sequence positive regulation of cell death chromatin modification methylation DNA methylation on cytosine DNA methylation involved in embryo development DNA methylation involved in gamete generation hypermethylation of CpG island S-adenosylhomocysteine metabolic process S-adenosylmethioninamine metabolic process cellular response to amino acid stimulus C-5 methylation of cytosine mitotic cell cycle aging response to toxic substance response to ionizing radiation response to lead ion neuron differentiation response to estradiol response to vitamin A response to antidepressant response to cocaine negative regulation of gene expression, epigenetic cellular response to ethanol cellular response to hypoxia hepatocyte apoptotic process

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DNMT3A Polyclonal Antibody

DNMT3A Antibody (PA3-16557) in IHC (P)

Product Details

PA3-16557

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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