Invitrogen

DOG-1 Recombinant Rabbit Monoclonal Antibody (SP31)

DOG-1 Antibody in Immunocytochemistry (ICC/IF)

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DOG-1 Antibody (MA5-16358) in ICC/IF

Immunofluorescence analysis of DOG-1 was performed using 70% confluent log phase PC-3 and DU 145 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. PC-3 cells were labeled with DOG-1 Monoclonal Antibody (Product # MA5-16358) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Pro... View More

DOG-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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DOG-1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of DOG-1 was observed in PC-3, HT-29, HCT 116 and COLO 205 cells in comparison to DU 145, HEK-293 cells using Anti-DOG-1 Monoclonal Antibody (SP31) (Product # MA5-16358) in Western blot. {RE}

Product Details

MA5-16358

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Immunocytochemistry (ICC/IF)

1:100

Flow Cytometry (Flow)

1:20

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Expression System

proprietary

Class

Recombinant Monoclonal

Type

Antibody

Clone

SP31

Immunogen

Synthetic peptides of human DOG-1 protein (aa 400-500).

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.057 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2537877

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is gastrointestinal stromal tumor.

Target Information

DOG-1, the novel marker, is expressed ubiquitously in gastrointestinal stromal tumors irrespective of kit or PDGFR alpha mutation status.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Identification of potential molecular targets for the treatment of cluster 1 human pheochromocytoma and paraganglioma via comprehensive proteomic characterization.

Clinical proteomics

Vit O,Talacko P,Musil Z,Hartmann I,Pacak K,Petrak J

MA5-16358 was used in Western Blot to identify potential drug targets in pheochromocytomas and paragangliomas (PPGLs) through comprehensive proteomic analysis, with a focus on integral membrane proteins upregulated in PPGLs and druggable non-membrane enzymes.

Mon Sep 25 00:00:00 UTC 2023

TMEM16A/ANO1 Inhibits Apoptosis Via Downregulation of Bim Expression.

Clinical cancer research : an official journal of the American Association for Cancer Research

Godse NR,Khan N,Yochum ZA,Gomez-Casal R,Kemp C,Shiwarski DJ,Seethala RS,Kulich S,Seshadri M,Burns TF,Duvvuri U

Published figure using DOG-1 recombinant monoclonal antibody (Product # MA5-16358) in Western Blot

Fri Dec 01 00:00:00 UTC 2017

TMEM16A/ANO1 is differentially expressed in HPV-negative versus HPV-positive head and neck squamous cell carcinoma through promoter methylation.

Scientific reports

Dixit R,Kemp C,Kulich S,Seethala R,Chiosea S,Ling S,Ha PK,Duvvuri U

MA5-16358 was used in immunohistochemistry to elucidate differential expression of TMEM16A/ANO1 in HPV-positive and HPV-negative head and neck squamous cell carcinoma through promoter methylation

Fri Nov 13 00:00:00 UTC 2015

Plexiform fibromyxoma (plexiform angiomyxoid myofibroblastic tumor) of stomach: an unusual presentation as a fistulating abscess.

International journal of surgical pathology

Lee PW,Yau DT,Lau PP,Chan JK

MA5-16358 was used in immunohistochemistry to report on a case of plexiform fibromyxoma of the stomach

Thu May 01 00:00:00 UTC 2014

Na+/Ca2+ exchangers regulate the migration and proliferation of human gastric myofibroblasts.

American journal of physiology. Gastrointestinal and liver physiology

Kemény LV,Schnúr A,Czepán M,Rakonczay Z,Gál E,Lonovics J,Lázár G,Simonka Z,Venglovecz V,Maléth J,Judák L,Németh IB,Szabó K,Almássy J,Virág L,Geisz A,Tiszlavicz L,Yule DI,Wittmann T,Varró A,Hegyi P

MA5-16358 was used in immunohistochemistry to study the mechanisms by which Na(+)/Ca(2+) exchangers modulate human gastric myofibroblast proliferation and migration

Tue Oct 15 00:00:00 UTC 2013

Contribution of DOG1 expression to the diagnosis of gastrointestinal stromal tumors.

Pathology, research and practice

Kara T,Serinsoz E,Arpaci RB,Gubur O,Orekici G,Ata A,Colak T,Arican A

MA5-16358 was used in immunohistochemistry to study the diagnostic value of the immunohistochemical expression of DOG1 in gastrointestinal stromal tumors

Mon Jul 01 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: anoctamin 1, calcium activated chloride channel; Anoctamin-1; Ca2+-activated Cl- channel; calcium activated chloride channel; Discovered on gastrointestinal stromal tumors protein 1; FLJ10261; oral cancer overexpressed 2; Oral cancer overexpressed protein 2; Transmembrane protein 16A; transmembrane protein 16A (eight membrane-spanning domains); Tumor-amplified and overexpressed sequence 2

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Gene Aliases: ANO1; DOG1; ORAOV2; TAOS2; TMEM16A

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UniProt ID: (Human) Q5XXA6

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Entrez Gene ID: (Human) 55107

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Function(s)

calcium activated cation channel activity intracellular calcium activated chloride channel activity voltage-gated chloride channel activity chloride channel activity protein binding iodide transmembrane transporter activity protein dimerization activity

Process(es)

cation transport chloride transport phospholipase C-activating G-protein coupled receptor signaling pathway multicellular organism development iodide transport ion transmembrane transport cellular response to heat positive regulation of insulin secretion involved in cellular response to glucose stimulus detection of temperature stimulus involved in sensory perception of pain cation transmembrane transport chloride transmembrane transport regulation of anion transmembrane transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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DOG-1 Recombinant Rabbit Monoclonal Antibody (SP31)

DOG-1 Antibody (MA5-16358) in ICC/IF

Product Details

MA5-16358

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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