Product References
The Intrinsically Disordered N Terminus in Atg12 from Yeast Is Necessary for the Functional Structure of the Protein.
International journal of molecular sciences
Popelka H,Lahiri V,Hawkins WD,da Veiga Leprevost F,Nesvizhskii AI,Klionsky DJ
A-6429 was used in Western Blot to provide evidence that the intrinsically disordered protein region (IDPR) is an indispensable part of the Atg12 protein from yeast.
Tue Oct 10 00:00:00 UTC 2023
Functional Analysis of the P-Type ATPases Apt2-4 from Cryptococcus neoformans by Heterologous Expression in Saccharomyces cerevisiae.
Journal of fungi (Basel, Switzerland)
Veit S,Laerbusch S,López-Marqués RL,Günther Pomorski T
A-6429 was used in Western Blot to identify C. neoformans Cdc50 as an essential subunit for Apt1-3p and provide a first insight into the molecular mechanisms underlying their physiological functions.
Sat Feb 04 00:00:00 UTC 2023
Eisosome protein Pil1 regulates mitochondrial morphology, mitophagy, and cell death in Saccharomyces cerevisiae.
The Journal of biological chemistry
Pal A,Paripati AK,Deolal P,Chatterjee A,Prasad PR,Adla P,Sepuri NBV
A-6429 was used in Western Blotting to suggest that Pil1, an eisosome protein, is a novel regulator of mitochondrial morphology, mitophagy, and cell death.
Tue Nov 01 00:00:00 UTC 2022
Eisosome protein Pil1 regulates mitochondrial morphology, mitophagy, and cell death in Saccharomyces cerevisiae.
The Journal of biological chemistry
Pal A,Paripati AK,Deolal P,Chatterjee A,Prasad PR,Adla P,Sepuri NBV
A-6429 was used in Western Blotting to suggest that Pil1, an eisosome protein, is a novel regulator of mitochondrial morphology, mitophagy, and cell death.
Tue Nov 01 00:00:00 UTC 2022
A method for the isolation and characterization of autophagic bodies from yeast provides a key tool to investigate cargos of autophagy.
The Journal of biological chemistry
Kawamata T,Makino S,Kagohashi Y,Sasaki M,Ohsumi Y
Published figure using DPM1 monoclonal antibody (Product # A-6429) in Western Blot
Thu Dec 01 00:00:00 UTC 2022
Post-transcriptional regulation of ATG1 is a critical node that modulates autophagy during distinct nutrient stresses.
Autophagy
Lahiri V,Metur SP,Hu Z,Song X,Mari M,Hawkins WD,Bhattarai J,Delorme-Axford E,Reggiori F,Tang D,Dengjel J,Klionsky DJ
A-6429 was used in Western Blotting to show that DDX3 regulates ULK1 expression post-transcriptionally, establishing mechanistic conservation and highlighting the power of yeast biology in uncovering regulatory mechanisms that can inform therapeutic approaches.
Fri Jul 01 00:00:00 UTC 2022
Follicular lymphoma-associated mutations in the V-ATPase chaperone VMA21 activate autophagy creating a targetable dependency.
Autophagy
Wang F,Yang Y,Boudagh G,Eskelinen EL,Klionsky DJ,Malek SN
A-6429 was used in Western Blot to report on novel mutations in the ER-resident chaperone VMA21, which is involved in V-ATPase assembly in 12% of FL.
Mon Aug 01 00:00:00 UTC 2022
Dimerization-dependent membrane tethering by Atg23 is essential for yeast autophagy.
Cell reports
Hawkins WD,Leary KA,Andhare D,Popelka H,Klionsky DJ,Ragusa MJ
A-6429 was used in Immunoprecipitation, Western Blot to show that Atg23 exists primarily as a homodimer, a conformation facilitated by a putative amphipathic helix.
Tue Apr 19 00:00:00 UTC 2022
Selectivity of mRNA degradation by autophagy in yeast.
Nature communications
Makino S,Kawamata T,Iwasaki S,Ohsumi Y
A-6429 was used in Western Blotting to demonstrate selective mRNA degradation by rapamycin-induced autophagy in yeast.
Mon Apr 19 00:00:00 UTC 2021
Human apolipoprotein L1 interferes with mitochondrial function in Saccharomyces cerevisiae.
Molecular medicine reports
Chidiac M,Daher J,Boeckstaens M,Poelvoorde P,Badran B,Marini AM,Khalaf R,Vanhamme L
A-6429 was used in Western Blotting to present evidence which suggests that APOL1 could be a physiological regulator of mitochondrial function.
Tue Sep 01 00:00:00 UTC 2020
Human apolipoprotein L1 interferes with mitochondrial function in Saccharomyces cerevisiae.
Molecular medicine reports
Chidiac M,Daher J,Boeckstaens M,Poelvoorde P,Badran B,Marini AM,Khalaf R,Vanhamme L
A-6429 was used in Western Blotting to present evidence which suggests that APOL1 could be a physiological regulator of mitochondrial function.
Tue Sep 01 00:00:00 UTC 2020
Mitochondrial Localization of the Yeast Forkhead Factor Hcm1.
International journal of molecular sciences
Rodríguez Colman MJ,Ros J,Cabiscol E
A-6429 was used in Western Blotting to show a new localization of Hcm1 and suggest that it acts as a mitochondrial transcription factor regulating the metabolism of this organelle.
Wed Dec 16 00:00:00 UTC 2020
Mitochondrial Localization of the Yeast Forkhead Factor Hcm1.
International journal of molecular sciences
Rodríguez Colman MJ,Ros J,Cabiscol E
A-6429 was used in Western Blotting to show a new localization of Hcm1 and suggest that it acts as a mitochondrial transcription factor regulating the metabolism of this organelle.
Wed Dec 16 00:00:00 UTC 2020
The mitochondrial phosphatidylserine decarboxylase Psd1 is involved in nitrogen starvation-induced mitophagy in yeast.
Journal of cell science
Vigié P,Cougouilles E,Bhatia-Kiššová I,Salin B,Blancard C,Camougrand N
A-6429 was used in Western Blotting to suggest that the mitophagy defect observed in δpsd1 yeast cells after nitrogen starvation may be due to a failure of Atg8 recruitment to mitochondria.
Wed Jan 02 00:00:00 UTC 2019
A switch element in the autophagy E2 Atg3 mediates allosteric regulation across the lipidation cascade.
Nature communications
Zheng Y,Qiu Y,Grace CRR,Liu X,Klionsky DJ,Schulman BA
A-6429 was used in Western Blotting to study the molecular mechanisms which underly Atg lipidation.
Fri Aug 09 00:00:00 UTC 2019
Mapping the Saccharomyces cerevisiae Spatial Proteome with High Resolution Using hyperLOPIT.
Methods in molecular biology (Clifton, N.J.)
Nightingale DJH,Oliver SG,Lilley KS
A-6429 was used in Western Blotting to describe a protocol for the unbiased and high-throughput study of protein subcellular localization in Saccharomyces cerevisiae via hyperLOPIT.
Mon Jun 22 00:00:00 UTC 2020
The exoribonuclease Xrn1 is a post-transcriptional negative regulator of autophagy.
Autophagy
Delorme-Axford E,Abernathy E,Lennemann NJ,Bernard A,Ariosa A,Coyne CB,Kirkegaard K,Klionsky DJ
Published figure using DPM1 monoclonal antibody (Product # A-6429) in Western Blot
Thu Oct 03 00:00:00 UTC 2019
Architecture of Lipid Droplets in Endoplasmic Reticulum Is Determined by Phospholipid Intrinsic Curvature.
Current biology : CB
Choudhary V,Golani G,Joshi AS,Cottier S,Schneiter R,Prinz WA,Kozlov MM
A-6429 was used in Western Blotting to study the mechanism by which cells regulate lipid droplet integration into the endoplasmic reticulum.
Mon Mar 19 00:00:00 UTC 2018
Regulation of lipid droplets by metabolically controlled Ldo isoforms.
The Journal of cell biology
Teixeira V,Johnsen L,Martínez-Montañés F,Grippa A,Buxó L,Idrissi FZ,Ejsing CS,Carvalho P
A-6429 was used in Immunofluorescence to support a model in which Ldo proteins modulate the activity of the seipin complex, thereby affecting lipid droplet (LD) properties, essential for energy homeostasis.
Tue Jan 02 00:00:00 UTC 2018
Formation of a Snf1-Mec1-Atg1 Module on Mitochondria Governs Energy Deprivation-Induced Autophagy by Regulating Mitochondrial Respiration.
Developmental cell
Yi C,Tong J,Lu P,Wang Y,Zhang J,Sun C,Yuan K,Xue R,Zou B,Li N,Xiao S,Dai C,Huang Y,Xu L,Li L,Chen S,Miao D,Deng H,Li H,Yu L
A-6429 was used in Western Blot to demonstrate that Mec1/ATR, a member of the DNA damage response pathway, is essential for glucose starvation-induced autophagy.
Mon Apr 10 00:00:00 UTC 2017
Structure and function of yeast Atg20, a sorting nexin that facilitates autophagy induction.
Proceedings of the National Academy of Sciences of the United States of America
Popelka H,Damasio A,Hinshaw JE,Klionsky DJ,Ragusa MJ
A-6429 was used in Western Blotting to demonstrate that Atg20 plays a critical role in the efficient induction of nonselective autophagy.
Tue Nov 21 00:00:00 UTC 2017
Lipid-dependent regulation of exocytosis in S. cerevisiae by OSBP homolog (Osh) 4.
Journal of cell science
Smindak RJ,Heckle LA,Chittari SS,Hand MA,Hyatt DM,Mantus GE,Sanfelippo WA,Kozminski KG
A-6429 was used in Western Blotting to describe a specific in vivo role for lipid binding by an OSBP-related protein in the process of polarized exocytosis, guiding our understanding of where and how OSBP and ORPs may function in more complex organisms.
Wed Nov 15 00:00:00 UTC 2017
Phosphorylation of Atg9 regulates movement to the phagophore assembly site and the rate of autophagosome formation.
Autophagy
Feng Y,Backues SK,Baba M,Heo JM,Harper JW,Klionsky DJ
Published figure using DPM1 monoclonal antibody (Product # A-6429) in Western Blot
Mon Dec 26 00:00:00 UTC 2016
An unrecognized function for COPII components in recruiting the viral replication protein BMV 1a to the perinuclear ER.
Journal of cell science
Li J,Fuchs S,Zhang J,Wellford S,Schuldiner M,Wang X
A6429 was used in immunocytochemistry and proximity ligation assay to demonstrate that some coat protein complex II proteins target proteins to the perinuclear endoplasmic reticulum membrane
Sat Oct 01 00:00:00 UTC 2016
A DNA-dependent protease involved in DNA-protein crosslink repair.
Cell
Stingele J,Schwarz MS,Bloemeke N,Wolf PG,Jentsch S
A-6429 was used in western blot to show that Wss1 is crucial for cell survival upon exposure to formaldehyde and topoisomerase 1-dependent DNA damage
Thu Jul 17 00:00:00 UTC 2014
Secretory vesicles deliver Cdc42p to sites of polarized growth in S. cerevisiae.
PloS one
Dighe SA,Kozminski KG
A-6429 was used in western blot to show that Cdc42p associates with secretory vesicles in vivo using S. cerevisiae
Mon Oct 19 00:00:00 UTC 2015
Proteomic profiling of autophagosome cargo in Saccharomyces cerevisiae.
PloS one
Suzuki K,Nakamura S,Morimoto M,Fujii K,Noda NN,Inagaki F,Ohsumi Y
A-6429 was used in western blot to describe a method for monitoring intact autophagosomes ex vivo.
Thu Jan 14 00:00:00 UTC 2016
Atg4 recycles inappropriately lipidated Atg8 to promote autophagosome biogenesis.
Autophagy
Nakatogawa H,Ishii J,Asai E,Ohsumi Y
A-6429 was used in Western Blotting to propose that Atg4 acts to compensate for intrinsic defects in the lipidation system, recycling Atg8-PE generated on inappropriate membranes.
Wed Feb 01 00:00:00 UTC 2012
Host acyl coenzyme A binding protein regulates replication complex assembly and activity of a positive-strand RNA virus.
Journal of virology
Zhang J,Diaz A,Mao L,Ahlquist P,Wang X
A-6429 was used in western blot to investigate how vesicular spherules and stacks of appressed double-membrane layers are formed.
Tue May 01 00:00:00 UTC 2012
Accumulation of an antidepressant in vesiculogenic membranes of yeast cells triggers autophagy.
PloS one
Chen J,Korostyshevsky D,Lee S,Perlstein EO
A-6429 was used in western blot to investigate the effects of sertraline/Zoloft® on the cell physiology of Saccharomyces cerevisiae.
Mon Nov 19 00:00:00 UTC 2012
Intersection of the multivesicular body pathway and lipid homeostasis in RNA replication by a positive-strand RNA virus.
Journal of virology
Wang X,Diaz A,Hao L,Gancarz B,den Boon JA,Ahlquist P
A-6429 was used in western blot to investigate the relationship between lipid synthesis and brome mosaic virus RNA replication.
Wed Jun 01 00:00:00 UTC 2011
Cbk1 kinase and Bck2 control MAP kinase activation and inactivation during heat shock.
Molecular biology of the cell
Kuravi VK,Kurischko C,Puri M,Luca FC
A-6429 was used in western blot to report that Cbk1 kinase regulates MAPK-dependent stress signaling
Thu Dec 01 00:00:00 UTC 2011
The reconstructed ancestral subunit a functions as both V-ATPase isoforms Vph1p and Stv1p in Saccharomyces cerevisiae.
Molecular biology of the cell
Finnigan GC,Hanson-Smith V,Houser BD,Park HJ,Stevens TH
A-6429 was used in western blot to identify the most recent common ancestor of Vph1p and Stv1p and assess its function in yeast
Thu Sep 01 00:00:00 UTC 2011
Identification of human gene products containing Pro-Pro-x-Tyr (PY) motifs that enhance glutathione and endocytotic marker uptake in yeast.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
Shi S,Notenboom S,Dumont ME,Ballatori N
Published figure using DPM1 monoclonal antibody (Product # A-6429) in Western Blot
Thu Apr 08 00:00:00 UTC 2010
Purification of yeast membranes and organelles by sucrose density gradient centrifugation.
Methods in molecular biology (Clifton, N.J.)
Chang J,Ruiz V,Vancura A
A-6429 was used in western blot to use differential and sucrose density gradient centrifugation to separate most yeast organelles in a single experiment
Tue Jan 06 00:00:00 UTC 2009
Proteasome-mediated protein processing by bidirectional degradation initiated from an internal site.
Nature structural & molecular biology
Piwko W,Jentsch S
A-6429 was used in western blot to analyze bidirectional degradation initiated from an internal site due to proteasome-mediated protein processing
Tue Aug 01 00:00:00 UTC 2006
A screen for genes of heme uptake identifies the FLC family required for import of FAD into the endoplasmic reticulum.
The Journal of biological chemistry
Protchenko O,Rodriguez-Suarez R,Androphy R,Bussey H,Philpott CC
A-6429 was used in western blot to identify the FLC family required for import of FAD into the endoplasmic reticulum by a screen for genes of heme uptake
Fri Jul 28 00:00:00 UTC 2006
Evidence for retro-translocation of pokeweed antiviral protein from endoplasmic reticulum into cytosol and separation of its activity on ribosomes from its activity on capped RNA.
Biochemistry
Parikh BA,Baykal U,Di R,Tumer NE
A-6429 was used in western blot to elucidate the cellular effects of pokeweed antiviral protein
Tue Feb 22 00:00:00 UTC 2005
C5a receptor oligomerization. II. Fluorescence resonance energy transfer studies of a human G protein-coupled receptor expressed in yeast.
The Journal of biological chemistry
Floyd DH,Geva A,Bruinsma SP,Overton MC,Blumer KJ,Baranski TJ
A-6429 was used in western blot to characterize fluorescence resonance energy transfer studies of a human G protein-coupled receptor expressed in yeast and C5a receptor oligomerization II
Fri Sep 12 00:00:00 UTC 2003
Mobilization of processed, membrane-tethered SPT23 transcription factor by CDC48(UFD1/NPL4), a ubiquitin-selective chaperone.
Cell
Rape M,Hoppe T,Gorr I,Kalocay M,Richly H,Jentsch S
A-6429 was used in western blot to elucidate SPT23 processing
Fri Nov 30 00:00:00 UTC 2001
Aut7p, a soluble autophagic factor, participates in multiple membrane trafficking processes.
The Journal of biological chemistry
Legesse-Miller A,Sagiv Y,Glozman R,Elazar Z
A-6429 was used in western blot to elucidate the contribution of Aut7p to different membrane trafficking processes
Fri Oct 20 00:00:00 UTC 2000
Desferrioxamine-mediated iron uptake in Saccharomyces cerevisiae. Evidence for two pathways of iron uptake.
The Journal of biological chemistry
Yun CW,Ferea T,Rashford J,Ardon O,Brown PO,Botstein D,Kaplan J,Philpott CC
A-6429 was used in western blot to identify new iron and AFT1-regulated genes in yeast
Fri Apr 07 00:00:00 UTC 2000
Novel localization of a Na+/H+ exchanger in a late endosomal compartment of yeast. Implications for vacuole biogenesis.
The Journal of biological chemistry
Nass R,Rao R
A-6429 was used in western blot to study the role of intracellular Na+/H+ exchangers using yeast
Fri Aug 14 00:00:00 UTC 1998
A novel membrane-bound glutathione S-transferase functions in the stationary phase of the yeast Saccharomyces cerevisiae.
The Journal of biological chemistry
Choi JH,Lou W,Vancura A
A-6429 was used in western blot to identify two genes that encode for glutathione S-transferases in Saccharomyces cerevisiae
Fri Nov 06 00:00:00 UTC 1998
VMA12 encodes a yeast endoplasmic reticulum protein required for vacuolar H+-ATPase assembly.
The Journal of biological chemistry
Jackson DD,Stevens TH
A-6429 was used in western blot to characterize Vma12p
Fri Oct 10 00:00:00 UTC 1997
