Invitrogen

DR3 Monoclonal Antibody (JD3), PE, eBioscience™

FIGURE: 1 / 1

DR3 Antibody (12-6603-41) in Flow

Staining of human DR3-transfected cells with Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (open histogram) or Anti-Human DR3 PE (filled histogram). Total viable cells were used for analysis.

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Product Details

12-6603-41

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 1 publication 1 publication

Product Specifications

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

JD3

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

AB_10870786

Product Specific Information

Description: The JD3 monoclonal antibody reacts with human DR3, also known as Apo-3, WSL-1, TRAMP, LARD, DDR3, and TR3. DR3, a novel death receptor is expressed at low level by monocytes and granulocytes. Interaction of DR3 with its ligand activates NF-kappa B pathway and induces apoptosis. Initially, DR3 was thought to be a receptor for TWEAK, but further studies have shown that TWEAK could induce apoptosis via receptors distinct from DR3. JD3 has been reported for use in flow cytometric analysis.

Applications Reported: The JD3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This JD3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells and human DR3-transfected cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

DR3/TNFRSF25 is a member of the TNF-receptor superfamily. This receptor is expressed preferentially in the tissues enriched in lymphocytes, and it may play a role in regulating lymphocyte homeostasis. This receptor has been shown to stimulate NF-kappa B activity and regulate cell apoptosis. The signal transduction of this receptor is mediated by various death domain containing adaptor proteins. Knockout studies in mice suggested the role of DR3/TNFRSF25 in the removal of self-reactive T cells in the thymus. Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported, most of which are potentially secreted molecules. The alternative splicing of this gene in B and T cells encounters a programmed change upon T-cell activation, which predominantly produces full-length, membrane bound isoforms, and is thought to be involved in controlling lymphocyte proliferation induced by T-cell activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

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Product References

CCL3 and MMP-9 are induced by TL1A during death receptor 3 (TNFRSF25)-dependent osteoclast function and systemic bone loss.

Bone

Collins FL,Williams JO,Bloom AC,Singh RK,Jordan L,Stone MD,McCabe LR,Wang ECY,Williams AS

12-6603 was used in Flow cytometry/Cell sorting to show that the DR3/TL1A pathway enhances human osteoclast function through the modulation of CCL3 and MMP-9 expression.

Sat Apr 01 00:00:00 UTC 2017

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