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Invitrogen
DR3 Monoclonal Antibody (JD3), eBioscience™
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Product Details
14-6603-82
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
JD3
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_468177
Product Specific Information
Description: The JD3 monoclonal antibody reacts with human DR3, also known as Apo-3, WSL-1, TRAMP, LARD, DDR3, and TR3. DR3, a novel death receptor is expressed at low level by monocytes and granulocytes. Interaction of DR3 with its ligand activates NF-kappa B pathway and induces apoptosis. Initially, DR3 was thought to be a receptor for TWEAK, but further studies have shown that TWEAK could induce apoptosis via receptors distinct from DR3. JD3 has been reported for use in flow cytometric analysis.
Applications Reported: The JD3 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The JD3 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells and human DR3-transfected cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
DR3/TNFRSF25 is a member of the TNF-receptor superfamily. This receptor is expressed preferentially in the tissues enriched in lymphocytes, and it may play a role in regulating lymphocyte homeostasis. This receptor has been shown to stimulate NF-kappa B activity and regulate cell apoptosis. The signal transduction of this receptor is mediated by various death domain containing adaptor proteins. Knockout studies in mice suggested the role of DR3/TNFRSF25 in the removal of self-reactive T cells in the thymus. Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported, most of which are potentially secreted molecules. The alternative splicing of this gene in B and T cells encounters a programmed change upon T-cell activation, which predominantly produces full-length, membrane bound isoforms, and is thought to be involved in controlling lymphocyte proliferation induced by T-cell activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Apo-3; apoptosis inducing receptor; Apoptosis-inducing receptor AIR; Apoptosis-mediating receptor DR3; Apoptosis-mediating receptor TRAMP; death domain receptor 3 soluble form; Death receptor 3; death receptor beta; LARD; Lymphocyte-associated receptor of death; Protein WSL; Protein WSL-1; RP4-650H14.2; Tumor necrosis factor receptor superfamily member 25; tumor necrosis factor receptor superfamily, member 12 (translocating chain-association membrane protein); tumor necrosis factor receptor superfamily, member 25
Gene Aliases: APO-3; APO3; DDR3; DR3; LARD; TNFRSF12; TNFRSF25; TR3; TRAMP; UNQ455/PRO779; WSL; WSL-1; WSL-LR; WSL1
UniProt ID: (Human) Q9UME0
Entrez Gene ID: (Human) 8718
apoptotic process
inflammatory response
immune response
signal transduction
cell surface receptor signaling pathway
multicellular organism development
response to lipopolysaccharide
tumor necrosis factor-mediated signaling pathway
regulation of cell proliferation
regulation of apoptotic process
positive regulation of MAPK cascade
apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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