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FIGURE: 1 / 1
DTL Antibody (PA5-88380) in WB
Western blot analysis of DTL in extracts of various cell lines. Samples were incubated with DTL Polyclonal antibody (Product # PA5-88380) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-88380
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 541-730 of human DTL (NP_0575323)
Conjugate
Form
Liquid
Concentration
1.52 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2804870
Product Specific Information
Immunogen sequence: AEACSESRNR VKRRLDSSCL ESVKQKCVKS CNCVTELDGQ VENLHLDLCC LAGNQEDLSK DSLGPTKSSK IEGAGTSISE PPSPISPYAS ESCGTLPLPL RPCGEGSEMV GKENSSPENK NWLLAMAAKR KAENPSPRSP SSQTPNSRRQ SGKTLPSPVT ITPSSMRKIC TYFHRKSQED FCGPEHSTEL; Positive Samples: Mouse testis, Mouse liver, Rat brain; Cellular Location: Chromosome, Cytoplasm, Nucleoplasmic side, Nucleus, Nucleus membrane, Peripheral membrane protein, centrosome, cytoskeleton, microtubule organizing center
Target Information
Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: denticleless E3 ubiquitin protein ligase homolog (Drosophila); denticleless homolog; Denticleless protein homolog; Lethal(2) denticleless protein homolog; Meth A RAMP; Meth A retinoic acid-regulated nuclear matrix-associated protein; Retinoic acid-regulated nuclear matrix-associated protein
Gene Aliases: 2810047L02Rik; 5730564G15Rik; Cdt2; Dtl; L2dtl; Ramp; RGD1310439
UniProt ID: (Mouse) Q3TLR7
Entrez Gene ID: (Mouse) 76843, (Rat) 305073
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