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DTYMK Polyclonal Antibody

DTYMK Antibody in Immunocytochemistry (ICC/IF)

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DTYMK Antibody (15360-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using DTYMK antibody 15360-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

DTYMK Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DTYMK Antibody in Immunoprecipitation (IP)

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Product Details

15360-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2400

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

DTYMK Fusion Protein Ag7606 (1-212 aa encoded by BC001827)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.15 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MAARRGALI VLEGVDRAGK STQSRKLVEA LCAAGHRAEL LRFPERSTEI GKLLSSYLQK KSDVEDHSVH LLFSANRWEQ VPLIKEKLSQ GVTLVVDRYA FSGVAFTGAK ENFSLDWCKQ PDVGLPKPDL VLFLQLQLAD AAKRGAFGHE RYENGAFQER ALRCFHQLMK DTTLNWKMVD ASKSIEAVHE DIRVLSEDAI RTATEKPLGE LWK (1-212 aa encoded by BC001827)

Target Information

Thymidylate kinase (DTYMK) catalyzes the phosphorylation of dTMP to form dTDP in the dTTP synthesis pathway for DNA synthesis. DTYMK is essential for DNA synthesis and is an important intermediate enzyme in the pathway of many pyrimidine analog drugs, including 5-Fluorouracil (5-FU), the chemotherapeutic drug of choice for several solid tumors. The human DTYMK gene was isolated via functional complementation of a Saccharomyces cerevisiae cell cycle mutant, cdc8. DTYMK mRNA levels and enzyme activities are regulated at the level of cell cycle and cell growth pathways.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Deoxythymidylate Kinase as a Promising Marker for Predicting Prognosis and Immune Cell Infiltration of Pan-cancer.

Frontiers in molecular biosciences

Lan T,Wang Y,Miao J,Guo H,Wang Z,Wang J,Zhang C,Yang P,Zhang Z,Dunmall LC,Wang Y

Wed Feb 14 00:00:00 UTC 2024

Targeting p300/CBP Attenuates Hepatocellular Carcinoma Progression through Epigenetic Regulation of Metabolism.

Cancer research

Cai LY,Chen SJ,Xiao SH,Sun QJ,Ding CH,Zheng BN,Zhu XY,Liu SQ,Yang F,Yang YX,Zhou B,Luo C,Zhang X,Xie WF

Mon Feb 15 00:00:00 UTC 2021

Targeting p300/CBP Attenuates Hepatocellular Carcinoma Progression through Epigenetic Regulation of Metabolism.

Cancer research

Cai LY,Chen SJ,Xiao SH,Sun QJ,Ding CH,Zheng BN,Zhu XY,Liu SQ,Yang F,Yang YX,Zhou B,Luo C,Zhang X,Xie WF

Mon Feb 15 00:00:00 UTC 2021

Inhibition of DTYMK significantly restrains the growth of HCC and increases sensitivity to oxaliplatin.

Cell death & disease

Sun F,Liu Y,Gong T,Pan Q,Xiang T,Zhao J,Tang Y,Chen H,Han Y,Song M,Huang Y,Li H,Chen Y,Yang C,Yang J,Wang Q,Li Y,He J,Weng D,Peng R,Xia J

Thu Nov 18 00:00:00 UTC 2021

Inhibition of DTYMK significantly restrains the growth of HCC and increases sensitivity to oxaliplatin.

Cell death & disease

Sun F,Liu Y,Gong T,Pan Q,Xiang T,Zhao J,Tang Y,Chen H,Han Y,Song M,Huang Y,Li H,Chen Y,Yang C,Yang J,Wang Q,Li Y,He J,Weng D,Peng R,Xia J

Thu Nov 18 00:00:00 UTC 2021

Distinct Classes of Flavonoids and Epigallocatechin Gallate, Polyphenol Affects an Oncogenic Mutant p53 Protein, Cell Growth and Invasion in a TNBC Breast Cancer Cell Line.

Cells

Kollareddy M,Martinez LA

Fri Apr 02 00:00:00 UTC 2021

Utilizing Optimized Tools to Investigate PTM Crosstalk: Identifying Potential PTM Crosstalk of Acetylated Mitochondrial Proteins.

Proteomes

Horita H,Law A,Middleton K

Tue May 22 00:00:00 UTC 2018

Utilizing Optimized Tools to Investigate PTM Crosstalk: Identifying Potential PTM Crosstalk of Acetylated Mitochondrial Proteins.

Proteomes

Horita H,Law A,Middleton K

Tue May 22 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: deoxythymidylate kinase (thymidylate kinase); dTMP kinase; Thymidylate kinase

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Gene Aliases: AU044245; CDC8; D10Ertd203e; DTYMK; mtmk; PP3731; Tmk; TMPK; TYMK; TYPK

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UniProt ID: (Human) P23919, (Mouse) P97930

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Entrez Gene ID: (Human) 1841, (Mouse) 21915

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Function(s)

thymidylate kinase activity ATP binding uridylate kinase activity nucleoside phosphate kinase activity nucleotide binding kinase activity transferase activity

Process(es)

dUDP biosynthetic process dTDP biosynthetic process dTTP biosynthetic process nucleotide biosynthetic process phosphorylation nucleotide phosphorylation nucleoside monophosphate phosphorylation cellular response to growth factor stimulus cell cycle cell proliferation nucleobase-containing small molecule interconversion response to estrogen myoblast differentiation response to cadmium ion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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DTYMK Polyclonal Antibody

DTYMK Antibody (15360-1-AP) in ICC/IF

Product Details

15360-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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