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Product Details
H00001843-M05
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
3A9
Immunogen
DUSP1 (NP_004408, 305 a.a. approximately 367 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Conjugate
Form
Liquid
Concentration
See Label
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Sequence of this protein is as follows: LLQFESQVLA PHCSAEAGSP AMAVLDRGTS TTTVFNFPVS IPVHSTNSAL SYLQSPITTS PSC
Target Information
The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CL 100; Dual specificity protein phosphatase 1; Dual specificity protein phosphatase hVH1; DUS1; EC 3.1.3.16; EC 3.1.3.48; MAP kinase phosphatase 1; Mitogen-activated protein kinase phosphatase 1; Protein-tyrosine phosphatase CL100; serine/threonine specific protein phosphatase
Gene Aliases: CL100; DUSP1; HVH1; MKP-1; MKP1; PTPN10; VH1
UniProt ID: (Human) P28562
Entrez Gene ID: (Human) 1843
inactivation of MAPK activity
endoderm formation
response to oxidative stress
response to light stimulus
response to estradiol
response to retinoic acid
cellular response to hormone stimulus
response to testosterone
peptidyl-tyrosine dephosphorylation
intracellular signal transduction
peptidyl-threonine dephosphorylation
response to hydrogen peroxide
regulation of apoptotic process
positive regulation of apoptotic process
negative regulation of apoptotic process
negative regulation of MAP kinase activity
negative regulation of MAPK cascade
response to glucocorticoid
negative regulation of meiotic cell cycle
response to cAMP
response to calcium ion
negative regulation of ERK1 and ERK2 cascade
mitotic cell cycle arrest
regulation of mitotic cell cycle spindle assembly checkpoint
negative regulation of DNA biosynthetic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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