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Invitrogen
DWARF 14 Polyclonal Antibody
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FIGURE: 1 / 3
DWARF 14 Antibody (PA5-119686) in IHC (P)
Immunohistochemistry (Paraffin) staining of paraffin- embedded rice tissue using DWARF 14 Polyclonal Antibody (Product # PA5-119686). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.(sodium citrate buffer (pH 6.0) for 20 mins.) . The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed... View More
Product Details
PA5-119686
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide within N-terminal Rice D14
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
TBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2913259
Product Specific Information
Positive Control: Rice.
Subcellular Location: Cytoplasm, Nucleus.
Target Information
Involved in strigolactone (SL) signaling pathway. May function downstream of SL synthesis, as a component of hormone signaling or as an enzyme that participates in the conversion of SL to the bioactive form. Strigolactones are hormones that inhibit tillering and shoot branching through the MAX-dependent pathway, contribute to the regulation of shoot architectural response to phosphate-limiting conditions and function as rhizosphere signal that stimulates hyphal branching of arbuscular mycorrhizal fungi and trigger seed germination of root parasitic weeds. Strigolactone-dependent association of D14 with D3 and D53 (a repressor of SL signaling) triggers D53 ubiquitination and degradation . Hydrolyzes the butenolide ring of SLs . A reaction product D-OH is trapped in the cavity of D14, inducing the interaction with SLR1, and probably with other proteins such as D3 and D53 . Contributes to the negative regulation of gibberellin signaling .
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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