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GenScript
DYKDDDDK Tag Recombinant Rabbit Monoclonal Antibody (114F12C8), HRP, MonoRab™
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FIGURE: 1 / 3
DYKDDDDK Tag Antibody (A01869-40) in WB
Sensitivity and specificity of MonoRab™ DYKDDDDK Tag Antibody [HRP], mAb, Rabbit (GenScript, A01869-40) to N-DYKDDDDK-tag Fusion Protein by Western blot. Loading: Lane 1 : N-terminal DYKDDDDK-tagged fusion protein (25 ng) Lane 2 : N-terminal DYKDDDDK-tagged fusion protein (10 ng) Lane 3 : N-terminal DYKDDDDK-tagged fusion protein (5 ng) Lane 4 : N-terminal DYKDDDDK-tagged fusion protein (1 ng) Primary Antibody: MonoRab™ DYKDDDDK Tag Antibody [HRP], mAb, Rabbit (GenScript, A01869-40) 1 µg/mL.
Product Details
A01869-40
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Host/Isotype
Rabbit
/ IgG
Expression System
Mammalian cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
114F12C8
Immunogen
DYKDDDDK synthetic peptide coupled to KLH
Conjugate
Form
Lyophilized
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.01% thimerosal
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
The rabbit immune system generates antibody diversity and optimizes affinity. GenScript utilizes MonoRab™ technology to generate the high affinity and specificity monoclonal rabbit antibodies. MonoRab™ DYKDDDDK Tag Antibody [HRP], mAb, Rabbit specific to DYKDDDDK tags placed at C-terminal, N-terminal and internal regions of fusion proteins. The antibody can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify DYKDDDDK fusion proteins in bacteria and mammalian cells.
Target Information
DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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