GenScript

DYKDDDDK Tag Monoclonal Antibody (5A8E5), FITC, THE™

DYKDDDDK Tag Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 1

DYKDDDDK Tag Antibody (A01632) in Flow

Flow cytometric analysis of transfected HEK-293 cell expressing DYKDDDDK-tagged protein using. THE™ Anti-DYKDDDDK [FITC] Monoclonal Antibody (GenScript, A01632; shaded histogram) or with a. negative control antibody (open histogram).

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Product Details

A01632

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

0.5-2 µg/mL

Flow Cytometry (Flow)

0.5-2 µg 1 x 10^6 cells

Miscellaneous PubMed (MISC)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Tag

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

5A8E5

Immunogen

A synthetic peptide DYKDDDDK coupled to KLH

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Lyophilized

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.

GenScript THE™ DYKDDDDK Tag Antibody [FITC], mAb, Mouse is FITC conjugated THE™ DYKDDDDK Tag Antibody, mAb, Mouse (A000187) purified from mice ascites by Protein A affinity column. This product is suitable for detecting the expression level of DYKDDDDK fusion proteins by various immunoassays. Absorption Wavelength: 492 nmEmission Wavelength: 518 nmFluorochrome/Protein Ratio: >3 moles FITC per mole antibody

Target Information

DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Controlling Depth of Cellular Quiescence by an Rb-E2F Network Switch.

Cell reports

Kwon JS,Everetts NJ,Wang X,Wang W,Della Croce K,Xing J,Yao G

Quiescence is a non-proliferative cellular state that is critical to tissue repair and regeneration. Although often described as the G0 phase quiescence is not a single homogeneous state. As cells remain quiescent for longer durations they move progressively deeper and display a reduced sensitivity to growth signals. Deep quiescent cells unlike senescent cells can still re-enter the cell cycle under physiological conditions. Mechanisms controlling quiescence depth are poorly understood

Tue Sep 26 00:00:00 UTC 2017

Nucleoporin Nup50 stabilizes closed conformation of armadillo repeat 10 in importin α5.

The Journal of biological chemistry

Pumroy RA,Nardozzi JD,Hart DJ,Root MJ,Cingolani G

The human genome encodes six isoforms of importin α that show greater than 60% sequence similarity and remarkable substrate specificity. The isoform importin α5 can bind phosphorylated cargos such as STAT1 and Epstein-Barr Virus Nuclear Antigen 1, as well as the influenza virus polymerase subunit PB2. In this work, we have studied the interaction of the nucleoporin Nup50 with importin α5. We show that the first 47 residues of Nup50 bind to the C terminus of importin α5 li

Fri Jan 13 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag

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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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