Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 2
DYKDDDDK Tag Antibody (701629RHRP) in WB
Western blot was performed using DYKDDDDK tag Recombinant Rabbit monoclonal antibody, HRP (8H8L17) (Product # 701629RHRP) by loading 30 µg modified whole cell extracts (1% SDS) of untransfected and transiently transfected HEK-293E lysates: Untransfected (Lane 1), empty vector control (Lane 2), HA-H3-DYKDDDDK (Lane 3), H3.3K4M-DYKDDDDK (Lane 4), H3.3K9M-DYKDDDDK (Lane 5), H3.3G34W-DYKDDDDK (Lane 6), DYKDDDDK-p65-HA (Lane 7), and 25 ng of Positope (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). R... View More
Product Details
701629RHRP
Product Specifications
Species Reactivity
Tag
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
8H8L17
Immunogen
Synthetic peptide (DYKDDDDK)
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with proprietary stabilizer
Contains
proprietary preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2942241
Product Specific Information
DYKDDDDK Tag recombinant rabbit monoclonal antibody conjugated with HRP specifically recognizes DYKDDDDK Tag at the C-terminus but not N-terminus of proteins.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity, lot-to-lot consistency, and broader immunoreactivity to diverse targets due to a larger rabbit immune repertoire.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support