Proteintech

DYNC1H1 Polyclonal Antibody

DYNC1H1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

DYNC1H1 Antibody (12345-1-AP) in ICC/IF

Immunofluorescent analysis of MCF-7 cells, using DYNC1H1 antibody 12345-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

DYNC1H1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Product Details

12345-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 25 publications 25 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:20-1:200

View 14 publications 14 publications

Immunoprecipitation (IP)

View 4 publications 4 publications

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Not applicable (N/A)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat, Zebrafish

Published species

Human, Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

DYNC1H1 Fusion Protein Ag2999 (590-989 aa encoded by BC021297)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MAQSIYGGR VDNEFDQRLL NTFLERLFTT RSFDSEFKLA CKVDGHKDIQ MPDGIRREEF VQWVELLPDT QTPSWLGLPN NAERVLLTTQ GVDMISKMLK MQMLEDEDDL AYAETEKKTR TDSTSDGRPA WMRTLHTTAS NWLHLIPQTL SHLKRTVENI KDPLFRFFER EVKMGAKLLQ DVRQDLADVV QVCEGKKKQT NYLRTLINEL VKGILPRSWS HYTVPAGMTV IQWVSDFSER IKQLQNISLA AASGGAKELK NIHVCLGGLF VPEAYITATR QYVAQANSWS LEELCLEVNV TTSQGATLDA CSFGVTGLKL QGATCNNNKL SLSNAISTAL PLTQLRWVKQ TNTEKKASVV TLPVYLNFTR ADLIFTVDFE IATKEDPRSF YERGVAVLCT E (590-989 aa encoded by BC021297)

Target Information

ZEB2, initially identified as Smad interacting-protein 1, is normally located in the nucleus and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs. Like the homologous ZEB1, ZEB2 inhibits the transcription of the E-cadherin gene and induces epithelial-mesenchymal transition, a genetic program controlling cell migration during embryonic development and wound healing, in vitro. ZEB2 can also protect cells from DNA damage-induced apoptosis, suggesting that its expression may contribute to tumor progression. Recent evidence has shown that ZEB2 is often observed in the cytoplasm in numerous cancer tissues, indicating that its localization may be regulated in normal and tumor tissues. Mutations in this gene are also associated with Hirschsprung disease/Mowat-Wilson syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Lou JX,Liu YY,Bai JS,Cheng Y,Zhang J,Liu CC,Zhou B

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Kinesin-1 Regulates Endocytic Trafficking of Classical Swine Fever Virus along Acetylated Microtubules.

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Lou JX,Liu YY,Bai JS,Cheng Y,Zhang J,Liu CC,Zhou B

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Kinesin-1 Regulates Endocytic Trafficking of Classical Swine Fever Virus along Acetylated Microtubules.

Journal of virology

Lou JX,Liu YY,Bai JS,Cheng Y,Zhang J,Liu CC,Zhou B

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A conditional null allele of Dync1h1 enables targeted analyses of dynein roles in neuronal length sensing.

Journal of cell science

Di Pizio A,Marvaldi L,Birling MC,Okladnikov N,Dupuis L,Fainzilber M,Rishal I

Wed Mar 01 00:00:00 UTC 2023

A conditional null allele of Dync1h1 enables targeted analyses of dynein roles in neuronal length sensing.

Journal of cell science

Di Pizio A,Marvaldi L,Birling MC,Okladnikov N,Dupuis L,Fainzilber M,Rishal I

Wed Mar 01 00:00:00 UTC 2023

Distinct dynein complexes defined by DYNLRB1 and DYNLRB2 regulate mitotic and male meiotic spindle bipolarity.

Nature communications

He S,Gillies JP,Zang JL,Córdoba-Beldad CM,Yamamoto I,Fujiwara Y,Grantham J,DeSantis ME,Shibuya H

Mon Mar 27 00:00:00 UTC 2023

Distinct dynein complexes defined by DYNLRB1 and DYNLRB2 regulate mitotic and male meiotic spindle bipolarity.

Nature communications

He S,Gillies JP,Zang JL,Córdoba-Beldad CM,Yamamoto I,Fujiwara Y,Grantham J,DeSantis ME,Shibuya H

Mon Mar 27 00:00:00 UTC 2023

Mapping rhodopsin trafficking in rod photoreceptors with quantitative super-resolution microscopy.

bioRxiv : the preprint server for biology

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A dominant negative mitofusin causes mitochondrial perinuclear clusters because of aberrant tethering.

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Science (New York, N.Y.)

So C,Menelaou K,Uraji J,Harasimov K,Steyer AM,Seres KB,Bucevičius J,Lukinavičius G,Möbius W,Sibold C,Tandler-Schneider A,Eckel H,Moltrecht R,Blayney M,Elder K,Schuh M

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Fri Jul 15 00:00:00 UTC 2022

Structuring and validating a prognostic model for low-grade gliomas based on the genes for plasma membrane tension.

Frontiers in neurology

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Tue Nov 22 00:00:00 UTC 2022

A New Monoclonal Antibody Enables BAR Analysis of Subcellular Importin β1 Interactomes.

Molecular & cellular proteomics : MCP

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Nano-graphene oxide depresses neurotransmission by blocking retrograde transport of mitochondria.

Journal of hazardous materials

Kang Y,Yin S,Liu J,Jiang Y,Huang Z,Chen L,Shao L

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Recycling of autophagosomal components from autolysosomes by the recycler complex.

Nature cell biology

Zhou C,Wu Z,Du W,Que H,Wang Y,Ouyang Q,Jian F,Yuan W,Zhao Y,Tian R,Li Y,Chen Y,Gao S,Wong CCL,Rong Y

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The mechanism of acentrosomal spindle assembly in human oocytes.

Science (New York, N.Y.)

Wu T,Dong J,Fu J,Kuang Y,Chen B,Gu H,Luo Y,Gu R,Zhang M,Li W,Dong X,Sun X,Sang Q,Wang L

Fri Nov 18 00:00:00 UTC 2022

Arf-like Protein 2 (ARL2) Controls Microtubule Neogenesis during Early Postnatal Photoreceptor Development.

Cells

Gerstner CD,Reed M,Dahl TM,Ying G,Frederick JM,Baehr W

Fri Dec 30 00:00:00 UTC 2022

Phosphorylation and Pin1 binding to the LIC1 subunit selectively regulate mitotic dynein functions.

The Journal of cell biology

Kumari A,Kumar C,Pergu R,Kumar M,Mahale SP,Wasnik N,Mylavarapu SVS

Mon Dec 06 00:00:00 UTC 2021

Phosphorylation and Pin1 binding to the LIC1 subunit selectively regulate mitotic dynein functions.

The Journal of cell biology

Kumari A,Kumar C,Pergu R,Kumar M,Mahale SP,Wasnik N,Mylavarapu SVS

Mon Dec 06 00:00:00 UTC 2021

A five-m6A regulatory gene signature is a prognostic biomarker in lung adenocarcinoma patients.

Aging

Wu X,Sheng H,Wang L,Xia P,Wang Y,Yu L,Lv W,Hu J

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Effect of conditional deletion of cytoplasmic dynein heavy chain DYNC1H1 on postnatal photoreceptors.

PloS one

Dahl TM,Reed M,Gerstner CD,Ying G,Baehr W

Tue Oct 12 00:00:00 UTC 2021

Microfilaments and microtubules alternately coordinate the multi-step endosomal trafficking of Classical Swine Fever Virus.

Journal of virology

Cheng Y,Lou JX,Liu CC,Liu YY,Chen XN,Liang XD,Zhang J,Yang Q,Go YY,Zhou B

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Affinity-based proteomics reveals novel binding partners for Rab46 in endothelial cells.

Scientific reports

Pedicini L,Wiktor SD,Simmons KJ,Money A,McKeown L

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DYNC1H1 regulates NSCLC cell growth and metastasis by IFN-γ-JAK-STAT signaling and is associated with an aberrant immune response.

Experimental cell research

Pan H,Chai W,Liu X,Yu T,Sun L,Yan M

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DYNC1H1 regulates NSCLC cell growth and metastasis by IFN-γ-JAK-STAT signaling and is associated with an aberrant immune response.

Experimental cell research

Pan H,Chai W,Liu X,Yu T,Sun L,Yan M

Wed Dec 01 00:00:00 UTC 2021

A septin GTPase scaffold of dynein-dynactin motors triggers retrograde lysosome transport.

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Maternal high sugar and fat diet benefits offspring brain function via targeting on the gut-brain axis.

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Conditional Deletion of Cytoplasmic Dynein Heavy Chain in Postnatal Photoreceptors.

Investigative ophthalmology & visual science

Dahl TM,Reed M,Gerstner CD,Baehr W

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Posterior Neocortex-Specific Regulation of Neuronal Migration by CEP85L Identifies Maternal Centriole-Dependent Activation of CDK5.

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Transgelin-2 and phosphoregulation of the LIC2 subunit of dynein govern mitotic spindle orientation.

Journal of cell science

Sharma A,Dagar S,Mylavarapu SVS

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A Conserved Role for Vezatin Proteins in Cargo-Specific Regulation of Retrograde Axonal Transport.

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A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.

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So C,Seres KB,Steyer AM,Mönnich E,Clift D,Pejkovska A,Möbius W,Schuh M

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EB1 and cytoplasmic dynein mediate protrusion dynamics for efficient 3-dimensional cell migration.

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EB1 and cytoplasmic dynein mediate protrusion dynamics for efficient 3-dimensional cell migration.

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Regulation of mitochondria-dynactin interaction and mitochondrial retrograde transport in axons.

eLife

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Palladin is a novel microtubule-associated protein responsible for spindle orientation.

Scientific reports

Zhang X,Chen X,Liu J,Xu X,Zhang Y,Ruan Z,Xie Y,Huang Q,Yin T,Chen Z,Chen S

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Palladin is a novel microtubule-associated protein responsible for spindle orientation.

Scientific reports

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Mon Feb 15 00:00:00 UTC 2016

Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway.

The Journal of cell biology

Inaba H,Goto H,Kasahara K,Kumamoto K,Yonemura S,Inoko A,Yamano S,Wanibuchi H,He D,Goshima N,Kiyono T,Hirotsune S,Inagaki M

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Wu CH,Zong Q,Du AL,Zhang W,Yao HC,Yu XQ,Wang YF

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Dynein Light Intermediate Chain 2 Facilitates the Metaphase to Anaphase Transition by Inactivating the Spindle Assembly Checkpoint.

PloS one

Mahale SP,Sharma A,Mylavarapu SV

Mon Jul 31 00:00:00 UTC 2017

Dynein Light Intermediate Chain 2 Facilitates the Metaphase to Anaphase Transition by Inactivating the Spindle Assembly Checkpoint.

PloS one

Mahale SP,Sharma A,Mylavarapu SV

Mon Jul 31 00:00:00 UTC 2017

Endophilin-A2 functions in membrane scission in clathrin-independent endocytosis.

Nature

Renard HF,Simunovic M,Lemière J,Boucrot E,Garcia-Castillo MD,Arumugam S,Chambon V,Lamaze C,Wunder C,Kenworthy AK,Schmidt AA,McMahon HT,Sykes C,Bassereau P,Johannes L

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Endophilin-A2 functions in membrane scission in clathrin-independent endocytosis.

Nature

Renard HF,Simunovic M,Lemière J,Boucrot E,Garcia-Castillo MD,Arumugam S,Chambon V,Lamaze C,Wunder C,Kenworthy AK,Schmidt AA,McMahon HT,Sykes C,Bassereau P,Johannes L

Thu Jan 22 00:00:00 UTC 2015

Tctex1d2 associates with short-rib polydactyly syndrome proteins and is required for ciliogenesis.

Cell cycle (Georgetown, Tex.)

Gholkar AA,Senese S,Lo YC,Capri J,Deardorff WJ,Dharmarajan H,Contreras E,Hodara E,Whitelegge JP,Jackson PK,Torres JZ

Mon Dec 28 00:00:00 UTC 2015

Tctex1d2 associates with short-rib polydactyly syndrome proteins and is required for ciliogenesis.

Cell cycle (Georgetown, Tex.)

Gholkar AA,Senese S,Lo YC,Capri J,Deardorff WJ,Dharmarajan H,Contreras E,Hodara E,Whitelegge JP,Jackson PK,Torres JZ

Mon Dec 28 00:00:00 UTC 2015

A Ras-like domain in the light intermediate chain bridges the dynein motor to a cargo-binding region.

eLife

Schroeder CM,Ostrem JM,Hertz NT,Vale RD

Wed Oct 01 00:00:00 UTC 2014

A Ras-like domain in the light intermediate chain bridges the dynein motor to a cargo-binding region.

eLife

Schroeder CM,Ostrem JM,Hertz NT,Vale RD

Wed Oct 01 00:00:00 UTC 2014

Depletion of pre-mRNA splicing factor Cdc5L inhibits mitotic progression and triggers mitotic catastrophe.

Cell death & disease

Mu R,Wang YB,Wu M,Yang Y,Song W,Li T,Zhang WN,Tan B,Li AL,Wang N,Xia Q,Gong WL,Wang CG,Zhou T,Guo N,Sang ZH,Li HY

Thu Mar 27 00:00:00 UTC 2014

Depletion of pre-mRNA splicing factor Cdc5L inhibits mitotic progression and triggers mitotic catastrophe.

Cell death & disease

Mu R,Wang YB,Wu M,Yang Y,Song W,Li T,Zhang WN,Tan B,Li AL,Wang N,Xia Q,Gong WL,Wang CG,Zhou T,Guo N,Sang ZH,Li HY

Thu Mar 27 00:00:00 UTC 2014

Proteome analysis of the sera from Chinese Parkinson's disease patients.

Neuroscience letters

Zhao X,Xiao WZ,Pu XP,Zhong LJ

Mon Jul 26 00:00:00 UTC 2010

Proteome analysis of the sera from Chinese Parkinson's disease patients.

Neuroscience letters

Zhao X,Xiao WZ,Pu XP,Zhong LJ

Mon Jul 26 00:00:00 UTC 2010

Analysis of a zebrafish dync1h1 mutant reveals multiple functions for cytoplasmic dynein 1 during retinal photoreceptor development.

Neural development

Insinna C,Baye LM,Amsterdam A,Besharse JC,Link BA

Thu Apr 22 00:00:00 UTC 2010

Analysis of a zebrafish dync1h1 mutant reveals multiple functions for cytoplasmic dynein 1 during retinal photoreceptor development.

Neural development

Insinna C,Baye LM,Amsterdam A,Besharse JC,Link BA

Thu Apr 22 00:00:00 UTC 2010

Analysis of a zebrafish dync1h1 mutant reveals multiple functions for cytoplasmic dynein 1 during retinal photoreceptor development.

Neural development

Insinna C,Baye LM,Amsterdam A,Besharse JC,Link BA

Thu Apr 22 00:00:00 UTC 2010

Analysis of a zebrafish dync1h1 mutant reveals multiple functions for cytoplasmic dynein 1 during retinal photoreceptor development.

Neural development

Insinna C,Baye LM,Amsterdam A,Besharse JC,Link BA

Thu Apr 22 00:00:00 UTC 2010

Bioinformatics

Protein Aliases: Cytoplasmic dynein 1 heavy chain 1; Cytoplasmic dynein heavy chain 1; cytoplasmic dynein heavy chain polypeptide 1; Dynein heavy chain, cytosolic; dynein heavy chain, retrograde transport; dynein, cytoplasmic 1, heavy chain 1; dynein, cytoplasmic, heavy chain 1; dynein, cytoplasmic, heavy polypeptide 1; legs at odd angle; MAP 1C; sprawling

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Gene Aliases: 9930018I23Rik; AI894280; CMT2O; DHC1; DHC1a; DNCH1; Dnchc1; DNCL; Dnec1; DNECL; DYHC; DYNC1H1; fk70a07; HL-3; KIAA0325; Loa; MAP1C; mKIAA0325; P22; SMALED1; Swl; wu:fk70a07

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UniProt ID: (Human) Q14204, (Rat) P38650, (Mouse) Q9JHU4

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Entrez Gene ID: (Human) 1778, (Rat) 29489, (Mouse) 13424, (Zebrafish) 335110

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Function(s)

microtubule motor activity protein binding ATP binding ATPase activity poly(A) RNA binding dynein light intermediate chain binding nucleotide binding motor activity

Process(es)

transport microtubule-based movement biological_process metabolic process cytoplasmic mRNA processing body assembly stress granule assembly G2/M transition of mitotic cell cycle ER to Golgi vesicle-mediated transport mitotic spindle organization antigen processing and presentation of exogenous peptide antigen via MHC class II establishment of spindle localization cilium movement central nervous system myelination myelination camera-type eye photoreceptor cell differentiation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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DYNC1H1 Polyclonal Antibody

DYNC1H1 Antibody (12345-1-AP) in ICC/IF

Product Details

12345-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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