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Invitrogen
Defensin alpha-3 Polyclonal Antibody
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Defensin alpha-3 Antibody (PA5-92407) in WB
Western blot analysis of Defensin alpha-3 in extracts of various cell lines. Samples were incubated with Defensin alpha-3 Polyclonal antibody (Product # PA5-92407) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Product Details
PA5-92407
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 20-94 of human DEFA3 (NP_0052081)
Conjugate
Form
Liquid
Concentration
0.61 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2806479
Product Specific Information
Immunogen sequence: EPLQARADEV AAAPEQIAAD IPEVVVSLAW DESLAPKHPG SRKNMDCYCR IPACIAGERR YGTCIYQGRL WAFCC; Positive Samples: MCF-7, 22Rv1, SW480
Target Information
Defensin alpha-1 is part of a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. Defensins are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The Defensin alpha-1 protein is encoded by the DFA1 gene and is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8, and this gene differs from defensin, alpha 3 by only one amino acid. The DFA1 gene and the gene encoding defensin, alpha 3 are both subject to copy number variation. Two transcript variants encoding different isoforms of Defensin alpha-1 have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: defensin 3, neutrophil-specific; Defensin, alpha 3; defensin, alpha 3, neutrophil-specific; HNP-3; HP-3; Neutrophil defensin 3; neutrophil peptide 3
Gene Aliases: DEF3; DEFA3; HNP-3; HNP3; HP-3; HP3
UniProt ID: (Human) P59666
Entrez Gene ID: (Human) 1668
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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