Invitrogen

Dendritic Cell Marker DCIR2 Monoclonal Antibody (33D1), eBioscience™

Dendritic Cell Marker DCIR2 Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 1

Dendritic Cell Marker DCIR2 Antibody (14-5884-82) in Flow

Staining of BALB/c splenocytes with 0.25 µg of Rat IgG2b K Isotype Control Purified (Product # 14-4031-82) (open histogram) or 0.25 µg of Anti-Mouse Dendritic Cell Marker DCIR2 Purified (filled histogram) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and Streptavidin PE (Product # 12-4317-87).Total viable cells were used for analysis.

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Product Details

14-5884-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

View 3 publications 3 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test

View 3 publications 3 publications

Functional Assay (Functional)

Assay-Dependent

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

33D1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_467676

Product Specific Information

Description: The 33D1 monoclonal antibody reacts with a mouse dendritic cell (DC)-specific surface marker, DCIR2. The nature and biological activity of DCIR2 is unknown. DCIR2 has been reported on a variety of dendritic cell subpopulations from mouse thymus, spleen, lymph node, and Peyer's patch. Bone marrow dendritic cells require GM-CSF to express DCIR2, and this expression is downregulated in the presence of IL-4. DCIR2 has been detected in vivo in brain dendritic cells post infection with Toxoplasma gondii.

Applications Reported: This 33D1 antibody has been reported for use in flow cytometric analysis, immunohistology staining of frozen tissue sections, and immunohistochemical staining. It has also been reported in complement-mediated cytotoxicity.

Applications Tested: The 33D1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. For detection of purified 33D1 it is recommended not to use goat anti-rat due to its low affinity for 33D1. Rather, we recommend using biotin mouse anti-rat (Product # 13-4813-85).

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The nature and biological activity of DCIR2 is unknown. DCIR2 has been reported on a variety of dendritic cell subpopulations from mouse thymus, spleen, lymph node, and Peyer's patch. Bone marrow dendritic cells require GM-CSF to express DCIR2, and this expression is downregulated in the presence of IL-4. DCIR2 has been detected in vivo in brain dendritic cells post infection with Toxoplasma gondii.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Protective role for Toll-like receptor-9 in the development of atherosclerosis in apolipoprotein E-deficient mice.

Arteriosclerosis, thrombosis, and vascular biology

Koulis C,Chen YC,Hausding C,Ahrens I,Kyaw TS,Tay C,Allen T,Jandeleit-Dahm K,Sweet MJ,Akira S,Bobik A,Peter K,Agrotis A

14-5884 was used in Immunohistochemistry to investigate whether TLR9 has a role in the development of atherosclerosis in apolipoprotein E-deficient mice.

Sat Mar 01 00:00:00 UTC 2014

Regulation of dendritic cell differentiation in bone marrow during emergency myelopoiesis.

Journal of immunology (Baltimore, Md. : 1950)

Liu H,Zhou J,Cheng P,Ramachandran I,Nefedova Y,Gabrilovich DI

14-5884 was used as a Control in experiments to demonstrate that dendritic cell differentiation during induced myelopoiesis can be differentially regulated by the Notch ligands Jagged-1 and Delta-like ligand 1.

Thu Aug 15 00:00:00 UTC 2013

Colon Macrophages Polarized by Commensal Bacteria Cause Colitis and Cancer through the Bystander Effect.

Translational oncology

Yang Y,Wang X,Huycke T,Moore DR,Lightfoot SA,Huycke MM

14-5884 was used in Immunohistochemistry on paraffin embedded tissues to investigate whether colon macrophages are key effectors for the bystander effect.

Wed Oct 23 00:00:00 UTC 2013

Comparable T helper 1 (Th1) and CD8 T-cell immunity by targeting HIV gag p24 to CD8 dendritic cells within antibodies to Langerin, DEC205, and Clec9A.

Proceedings of the National Academy of Sciences of the United States of America

Idoyaga J,Lubkin A,Fiorese C,Lahoud MH,Caminschi I,Huang Y,Rodriguez A,Clausen BE,Park CG,Trumpfheller C,Steinman RM

Tue Feb 08 00:00:00 UTC 2011

Impact of a probiotic fermented milk in the gut ecosystem and in the systemic immunity using a non-severe protein-energy-malnutrition model in mice.

BMC gastroenterology

Maldonado Galdeano C,Novotny Núñez I,de Moreno de LeBlanc A,Carmuega E,Weill R,Perdigón G

14-5884 was used in Immunofluorescence to evaluate the effects of a probiotic fermented milk as a complement of a re-nutrition diet and for its protection against Salmonella.

Thu May 26 00:00:00 UTC 2011

Targeting Stat3 in the myeloid compartment drastically improves the in vivo antitumor functions of adoptively transferred T cells.

Cancer research

Herrmann A,Kortylewski M,Kujawski M,Zhang C,Reckamp K,Armstrong B,Wang L,Kowolik C,Deng J,Figlin R,Yu H

14-5884 was used in Flow cytometry/Cell sorting to study the effects of inhibiting the signal transducer and activator of transcription 3 (Stat3) on antitumour functions.

Fri Oct 01 00:00:00 UTC 2010

Combined treatment with triptolide and rapamycin prolongs graft survival in a mouse model of cardiac transplantation.

Transplant international : official journal of the European Society for Organ Transplantation

Liu Y,Chen Y,Liu FQ,Lamb JR,Tam PK

14-5884 was used in Immunohistochemistry to study the effects of triptolide and rapamycin combination therapy on the survival of cardiac allografts.

Thu May 01 00:00:00 UTC 2008

Effect of the administration of a fermented milk containing Lactobacillus casei DN-114001 on intestinal microbiota and gut associated immune cells of nursing mice and after weaning until immune maturity.

BMC immunology

de Moreno de LeBlanc A,Dogi CA,Galdeano CM,Carmuega E,Weill R,Perdigón G

14-5884 was used in Immunohistochemistry on paraffin embedded tissues to evaluate the effect of the administration of a probiotic fermented milk on the intestinal bacterial population and on parameters of the gut immune system.

Fri Jun 13 00:00:00 UTC 2008

Differential antigen processing by dendritic cell subsets in vivo.

Science (New York, N.Y.)

Dudziak D,Kamphorst AO,Heidkamp GF,Buchholz VR,Trumpfheller C,Yamazaki S,Cheong C,Liu K,Lee HW,Park CG,Steinman RM,Nussenzweig MC

Fri Jan 05 00:00:00 UTC 2007

Bioinformatics

Protein Aliases: C-type lectin domain family 4, member a4; DC Marker; dendritic cell inhibitory receptor 2

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Gene Aliases: Dcir2

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Entrez Gene ID: (Mouse) 474145

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Function(s)

carbohydrate derivative binding

Process(es)

biological_process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Dendritic Cell Marker DCIR2 Monoclonal Antibody (33D1), eBioscience™

Dendritic Cell Marker DCIR2 Antibody (14-5884-82) in Flow

Product Details

14-5884-82

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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