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Denosumab Antibody (A02102-40) in ELISA
Anti-Denosumab Antibody (9A10) (GenScript, A02102-40) blocks Denosumab binding with Human RANKL recombinant protein. Coating antigen: Denosumab 1 µg/mL. RANKL final concentration: 12.5 ng/mL. Anti-Denosumab antibody dilutions start from 30 μg/mL. IC50= 0.893 µg/mL.
Product Details
A02102-40
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
9A10
Immunogen
Denosumab
Conjugate
Form
Lyophilized
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
Product Specific Information
Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.
Competitive ELISA recommended dilution: 5-30 µg/mL
The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
Target Information
Denosumab is a novel, fully human IgG2 monoclonal antibody specific to receptor activator of nuclear factor kappa-B ligand (RANKL), suppresses bone resorption markers in patients with a variety of metastatic tumors and is being investigated in multiple clinical trials for the prevention and treatment of bone metastases. Chemically, it consists of 2 heavy and 2 light chains. Each light chain consists of 215 amino acids. Each heavy chain consists of 448 amino acids with 4 intramolecular disulfides. FDA approved on June 1, 2010.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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