Invitrogen

Desmocollin 2/3 Monoclonal Antibody (7G6)

Desmocollin 2/3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Desmocollin 2/3 Antibody (32-6200) in IHC (P)

Immunohistochemistry analysis of Desmocollin-2/3 showing staining in the membrane of paraffin-embedded human heart tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room tempera... View More

Desmocollin 2/3 Antibody in Western Blot (WB)

Desmocollin 2/3 Antibody in Immunocytochemistry (ICC/IF)

Desmocollin 2/3 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Desmocollin 2/3 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

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Product Details

32-6200

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Immunoprecipitation (IP)

5-10 µg

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

7G6

Immunogen

Human desmocollin-2 extracellular domain.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533090

Target Information

This gene encodes a member of the desmocollin protein subfamily. Desmocollins, along with desmogleins, are cadherin-like transmembrane glycoproteins that are major components of the desmosome. Desmosomes are cell-cell junctions that help resist shearing forces and are found in high concentrations in cells subject to mechanical stress. This gene is found in a cluster with other desmocollin family members on chromosome 18. Mutations in this gene are associated with arrhythmogenic right ventricular dysplasia-11, and reduced protein expression has been described in several types of cancer. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Plakophilin 2 regulates intestinal barrier function by modulating protein kinase C activity in vitro.

Tissue barriers

Nagler S,Ghoreishi Y,Kollmann C,Kelm M,Gerull B,Waschke J,Burkard N,Schlegel N

Published figure using Desmocollin 2/3 monoclonal antibody (Product # 32-6200) in Western Blot

Mon Oct 02 00:00:00 UTC 2023

The Actin-Binding Protein α-Adducin Modulates Desmosomal Turnover and Plasticity.

The Journal of investigative dermatology

Hiermaier M,Kliewe F,Schinner C,Stüdle C,Maly IP,Wanuske MT,Rötzer V,Endlich N,Vielmuth F,Waschke J,Spindler V

32-6200 was used in Western Blot to demonstrate the plasticity of desmosomal molecules in response to mechanical stimuli and unravel a mechanism of how the actin cytoskeleton indirectly shapes intercellular adhesion by restricting the membrane mobility of desmosomal molecules.

Sat May 01 00:00:00 UTC 2021

Regulation of intestinal epithelial intercellular adhesion and barrier function by desmosomal cadherin desmocollin-2.

Molecular biology of the cell

Raya-Sandino A,Luissint AC,Kusters DHM,Narayanan V,Flemming S,Garcia-Hernandez V,Godsel LM,Green KJ,Hagen SJ,Conway DE,Parkos CA,Nusrat A

Published figure using Desmocollin 2/3 monoclonal antibody (Product # 32-6200) in Western Blot

Thu Apr 15 00:00:00 UTC 2021

Desmosomal proteins of DSC2 and PKP1 promote cancer cells survival and metastasis by increasing cluster formation in circulatory system.

Science advances

Li K,Wu R,Zhou M,Tong H,Luo KQ

Published figure using Desmocollin 2/3 monoclonal antibody (Product # 32-6200) in Western Blot

Fri Oct 01 00:00:00 UTC 2021

Desmocollin-2 promotes intestinal mucosal repair by controlling integrin-dependent cell adhesion and migration.

Molecular biology of the cell

Flemming S,Luissint AC,Kusters DHM,Raya-Sandino A,Fan S,Zhou DW,Hasegawa M,Garcia-Hernandez V,García AJ,Parkos CA,Nusrat A

32-6200 was used in Immunohistochemistry to demonstrate that epithelial Dsc2 is a key contributor to intestinal mucosal wound healing in vivo.

Sun Mar 15 00:00:00 UTC 2020

Desmocollin-2 promotes intestinal mucosal repair by controlling integrin-dependent cell adhesion and migration.

Molecular biology of the cell

Flemming S,Luissint AC,Kusters DHM,Raya-Sandino A,Fan S,Zhou DW,Hasegawa M,Garcia-Hernandez V,García AJ,Parkos CA,Nusrat A

32-6200 was used in Immunohistochemistry to demonstrate that epithelial Dsc2 is a key contributor to intestinal mucosal wound healing in vivo.

Sun Mar 15 00:00:00 UTC 2020

Desmocollin-2 promotes intestinal mucosal repair by controlling integrin-dependent cell adhesion and migration.

Molecular biology of the cell

Flemming S,Luissint AC,Kusters DHM,Raya-Sandino A,Fan S,Zhou DW,Hasegawa M,Garcia-Hernandez V,García AJ,Parkos CA,Nusrat A

32-6200 was used in Immunohistochemistry to demonstrate that epithelial Dsc2 is a key contributor to intestinal mucosal wound healing in vivo.

Sun Mar 15 00:00:00 UTC 2020

Desmocollin-2 promotes intestinal mucosal repair by controlling integrin-dependent cell adhesion and migration.

Molecular biology of the cell

Flemming S,Luissint AC,Kusters DHM,Raya-Sandino A,Fan S,Zhou DW,Hasegawa M,Garcia-Hernandez V,García AJ,Parkos CA,Nusrat A

32-6200 was used in Immunohistochemistry to demonstrate that epithelial Dsc2 is a key contributor to intestinal mucosal wound healing in vivo.

Sun Mar 15 00:00:00 UTC 2020

TGF-β1 affects cell-cell adhesion in the heart in an NCAM1-dependent mechanism.

Journal of molecular and cellular cardiology

Ackermann MA,Petrosino JM,Manring HR,Wright P,Shettigar V,Kilic A,Janssen PML,Ziolo MT,Accornero F

32-6200 was used in Immunofluorescence in immunohistochemistry on frozen tissues to investigate the molecular function of Transforming Growth Factor beta 1 in controlling cell-cell adhesion in the heart.

Wed Nov 01 00:00:00 UTC 2017

Inflammation-induced desmoglein-2 ectodomain shedding compromises the mucosal barrier.

Molecular biology of the cell

Kamekura R,Nava P,Feng M,Quiros M,Nishio H,Weber DA,Parkos CA,Nusrat A

32-6200 was used in Western Blotting to reveal a response pathway in which inflammation-induced modification of columnar epithelial cell cadherins decreases intercellular adhesion while enhancing cellular proliferation.

Tue Sep 15 00:00:00 UTC 2015

Desmocollin‑2 affects the adhesive strength and cytoskeletal arrangement in esophageal squamous cell carcinoma cells.

Molecular medicine reports

Fang WK,Liao LD,Zeng FM,Zhang PX,Wu JY,Shen J,Xu LY,Li EM

32-6200 was used in immunocytochemistry and western blot to study the role of desmocollin-2 in adhesive strength and cytoskeleton architecture in esophageal squamous cell carcinoma

Sat Nov 01 00:00:00 UTC 2014

Desmocollin‑2 affects the adhesive strength and cytoskeletal arrangement in esophageal squamous cell carcinoma cells.

Molecular medicine reports

Fang WK,Liao LD,Zeng FM,Zhang PX,Wu JY,Shen J,Xu LY,Li EM

32-6200 was used in immunocytochemistry and western blot to study the role of desmocollin-2 in adhesive strength and cytoskeleton architecture in esophageal squamous cell carcinoma

Sat Nov 01 00:00:00 UTC 2014

Homozygous founder mutation in desmocollin-2 (DSC2) causes arrhythmogenic cardiomyopathy in the Hutterite population.

Circulation. Cardiovascular genetics

Gerull B,Kirchner F,Chong JX,Tagoe J,Chandrasekharan K,Strohm O,Waggoner D,Ober C,Duff HJ

32-6200 was used in immunohistochemistry (paraffin) to discuss the mutation that result in arrhythmogenic cardiomyopathy and cardiocutaneous syndromes in the Hutterites.

Thu Aug 01 00:00:00 UTC 2013

Hailey-Hailey disease and tight junctions: Claudins 1 and 4 are regulated by ATP2C1 gene encoding Ca(2+) /Mn(2+) ATPase SPCA1 in cultured keratinocytes.

Experimental dermatology

Raiko L,Siljamäki E,Mahoney MG,Putaala H,Suominen E,Peltonen J,Peltonen S

Wed Aug 01 00:00:00 UTC 2012

The keratin-binding protein Albatross regulates polarization of epithelial cells.

The Journal of cell biology

Sugimoto M,Inoko A,Shiromizu T,Nakayama M,Zou P,Yonemura S,Hayashi Y,Izawa I,Sasoh M,Uji Y,Kaibuchi K,Kiyono T,Inagaki M

32-6200 was used in western blot to elucidate how the keratin intermediate filament network is involved in the establishment and maintenance of cell polarity

Mon Oct 06 00:00:00 UTC 2008

Characterization of a novel type of adherens junction in meningiomas and the derived cell line HBL-52.

Cell and tissue research

Akat K,Bleck CK,Lee YM,Haselmann-Weiss U,Kartenbeck J

32-6200 was used in immunocytochemistry identify and characterize a new type of adherens junction in human meningiomas

Fri Feb 01 00:00:00 UTC 2008

C-terminal truncation of connexin43 changes number, size, and localization of cardiac gap junction plaques.

Circulation research

Maass K,Shibayama J,Chase SE,Willecke K,Delmar M

32-6200 was used in Western Blotting to conclude that the carboxyl-terminal domain of Cx43 (Cx43CT) is involved in regulating the localization, number and size of Cx43 plaques in vivo.

Fri Dec 07 00:00:00 UTC 2007

Remodeling of myocyte gap junctions in arrhythmogenic right ventricular cardiomyopathy due to a deletion in plakoglobin (Naxos disease).

Heart rhythm

Kaplan SR,Gard JJ,Protonotarios N,Tsatsopoulou A,Spiliopoulou C,Anastasakis A,Squarcioni CP,McKenna WJ,Thiene G,Basso C,Brousse N,Fontaine G,Saffitz JE

32-6200 was used in immunohistochemistry - frozen section to investigate the effect of the plakoglobin mutation in Naxos disease on connexin43

Sat May 01 00:00:00 UTC 2004

Structural and molecular pathology of the heart in Carvajal syndrome.

Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology

Kaplan SR,Gard JJ,Carvajal-Huerta L,Ruiz-Cabezas JC,Thiene G,Saffitz JE

32-6200 was used in immunohistochemistry - paraffin section to describe the pathology of Carvajal syndrome

Tue Nov 16 00:00:00 UTC 2004

Bioinformatics

Protein Aliases: ARVC10; ARVD10; Cadherin family member 2; Cadherin family member 3; CDHF5; CDHF6; CMD1BB; Desmocollin-2; Desmocollin-3; Desmocollin-4; desmoglein-2; desmoglein-3; Desmosomal glycoprotein II; desmosomal glycoprotein II/III; Desmosomal glycoprotein III; HDGC; HT-CP; PVA

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Gene Aliases: ARVD11; CDHF2; CDHF3; DG2; DGII/III; DSC; DSC1; DSC2; DSC3; DSC4; HT-CP

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UniProt ID: (Human) Q02487, (Human) Q14574

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Entrez Gene ID: (Human) 1824, (Human) 1825

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Function(s)

calcium ion binding protein binding cell adhesive protein binding involved in bundle of His cell-Purkinje myocyte communication

Process(es)

cell adhesion homophilic cell adhesion via plasma membrane adhesion molecules cellular response to starvation cardiac muscle cell-cardiac muscle cell adhesion bundle of His cell-Purkinje myocyte adhesion involved in cell communication regulation of heart rate by cardiac conduction regulation of ventricular cardiac muscle cell action potential

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Desmocollin 2/3 Monoclonal Antibody (7G6)

Desmocollin 2/3 Antibody (32-6200) in IHC (P)

Product Details

32-6200

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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