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Desmoplakin Polyclonal Antibody

View all (23) Desmoplakin antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (13)

Desmoplakin Antibody in Immunocytochemistry (ICC/IF)

Desmoplakin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 13

Desmoplakin Antibody (25318-1-AP) in ICC/IF

Immunofluorescent analysis of A431 cells using 25318-1-AP (Desmoplakin antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Desmoplakin Antibody in Immunocytochemistry (ICC/IF)

Desmoplakin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Desmoplakin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Desmoplakin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Desmoplakin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Desmoplakin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Desmoplakin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Desmoplakin Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

Desmoplakin Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

Desmoplakin Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

Desmoplakin Antibody in Western Blot (WB)

Desmoplakin Antibody in Western Blot (WB)

Desmoplakin Antibody in Western Blot (WB)

Product Image Gallery

Desmoplakin Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of A431 cells using 25318-1-AP (Desmoplakin antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Desmoplakin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded Mouse heart tissue slide using (Product # 25318-1-AP) Desmoplakin antibody at dilution of 1:5,000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Desmoplakin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded Mouse heart tissue slide using (Product # 25318-1-AP) Desmoplakin antibody at dilution of 1:5,000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Desmoplakin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded Rat heart tissue slide using (Product # 25318-1-AP) Desmoplakin antibody at dilution of 1:5,000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Desmoplakin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded Rat heart tissue slide using (Product # 25318-1-AP) Desmoplakin antibody at dilution of 1:5,000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Desmoplakin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 25318-1-AP ( Desmoplakin antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Desmoplakin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 25318-1-AP ( Desmoplakin antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Desmoplakin Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using (Product # 25318-1-AP) (Desmoplakin antibody), at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Desmoplakin Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — Immunofluorescent analysis of ( 4% PFA) fixed mouse heart tissue using 25318-1-AP (Desmoplakin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Desmoplakin Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — Immunofluorescent analysis of ( 4% PFA) fixed mouse heart tissue using 25318-1-AP (Desmoplakin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Desmoplakin Antibody in Western Blot (WB) — Mouse heart tissue were subjected to SDS PAGE followed by western blot with 25318-1-AP (Desmoplakin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Desmoplakin Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 25318-1-AP (Desmoplakin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

Desmoplakin Antibody in Western Blot (WB) — WB result of Desmoplakin antibody (25318-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Desmoplakin transfected HepG2 cells.

Product Details

25318-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:10-1:100

-

Immunocytochemistry (ICC/IF)

1:10-1:100

View 3 publications 3 publications

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Desmoplakin Fusion Protein Ag18065 (1-350 aa encoded by BC140802)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.4 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody detects both DP1 (280-330 kDa) and DP2 (240-260 kDa).

Immunogen sequence: MSCNGGSHP RINTLGRMIR AESGPDLRYE VTSGGGGTSR MYYSRRGVIT DQNSDGYCQT GTMSRHQNQN TIQELLQNCS DCLMRAELIV QPELKYGDGI QLTRSRELDE CFAQANDQME ILDSLIREMR QMGQPCDAYQ KRLLQLQEQM RALYKAISVP RVRRASSKGG GGYTCQSGSG WDEFTKHVTS ECLGWMRQQR AEMDMVAWGV DLASVEQHIN SHRGIHNSIG DYRWQLDKIK ADLREKSAIY QLEEEYENLL KASFERMDHL RQLQNIIQAT SREIMWINDC EEEELLYDWS DKNTNIAQKQ EAFSIRMSQL EVKEKELNKL KQESDQLVLN QHPASDKIEA Y (1-350 aa encoded by BC140802)

Target Information

Desmoplakin (DSP) is a major high molecular weight protein of the desmosomes. It is involved in the organization of the desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains and in the anchoring of intermediate filaments to the desmosomes. Desmoplakin mutations are associated with Keratoderma and cardiomyopathy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Cancer gene therapy

Wang J,Huang TJ,Mei Y,Luo FF,Xie DH,Peng LX,Liu BQ,Fan ML,Zhang JB,Zheng ST,Qian CN,Huang BJ

Wed Feb 01 00:00:00 UTC 2023

Cis-regulatory chromatin loops analysis identifies GRHL3 as a master regulator of surface epithelium commitment.

Science advances

Huang H,Liu J,Li M,Guo H,Zhu J,Zhu L,Wu S,Mo K,Huang Y,Tan J,Chen C,Wang B,Yu Y,Wang L,Liu Y,Ouyang H

Fri Jul 15 00:00:00 UTC 2022

CYLD deubiquitinates plakoglobin to promote Cx43 membrane targeting and gap junction assembly in the heart.

Cell reports

Xie W,Gao S,Yang Y,Li H,Zhou J,Chen M,Yang S,Zhang Y,Zhang L,Meng X,Xie S,Liu M,Li D,Chen Y,Zhou J

Tue Dec 27 00:00:00 UTC 2022

CYLD deubiquitinates plakoglobin to promote Cx43 membrane targeting and gap junction assembly in the heart.

Cell reports

Xie W,Gao S,Yang Y,Li H,Zhou J,Chen M,Yang S,Zhang Y,Zhang L,Meng X,Xie S,Liu M,Li D,Chen Y,Zhou J

Tue Dec 27 00:00:00 UTC 2022

CYLD deubiquitinates plakoglobin to promote Cx43 membrane targeting and gap junction assembly in the heart.

Cell reports

Xie W,Gao S,Yang Y,Li H,Zhou J,Chen M,Yang S,Zhang Y,Zhang L,Meng X,Xie S,Liu M,Li D,Chen Y,Zhou J

Tue Dec 27 00:00:00 UTC 2022

CYLD deubiquitinates plakoglobin to promote Cx43 membrane targeting and gap junction assembly in the heart.

Cell reports

Xie W,Gao S,Yang Y,Li H,Zhou J,Chen M,Yang S,Zhang Y,Zhang L,Meng X,Xie S,Liu M,Li D,Chen Y,Zhou J

Tue Dec 27 00:00:00 UTC 2022

CYLD deubiquitinates plakoglobin to promote Cx43 membrane targeting and gap junction assembly in the heart.

Cell reports

Xie W,Gao S,Yang Y,Li H,Zhou J,Chen M,Yang S,Zhang Y,Zhang L,Meng X,Xie S,Liu M,Li D,Chen Y,Zhou J

Tue Dec 27 00:00:00 UTC 2022

CYLD deubiquitinates plakoglobin to promote Cx43 membrane targeting and gap junction assembly in the heart.

Cell reports

Xie W,Gao S,Yang Y,Li H,Zhou J,Chen M,Yang S,Zhang Y,Zhang L,Meng X,Xie S,Liu M,Li D,Chen Y,Zhou J

Tue Dec 27 00:00:00 UTC 2022

Inhibition of the Growth of Breast Cancer-Associated Brain Tumors by the Osteocyte-Derived Conditioned Medium.

Cancers

Sano T,Sun X,Feng Y,Liu S,Hase M,Fan Y,Zha R,Wu D,Aryal UK,Li BY,Sudo A,Yokota H

Wed Mar 03 00:00:00 UTC 2021

Overexpression of Lrp5 enhanced the anti-breast cancer effects of osteocytes in bone.

Bone research

Liu S,Wu D,Sun X,Fan Y,Zha R,Jalali A,Feng Y,Li K,Sano T,Vike N,Li F,Rispoli J,Sudo A,Liu J,Robling A,Nakshatri H,Li BY,Yokota H

Tue Jul 06 00:00:00 UTC 2021

Long noncoding RNA LYPLAL1-AS1 regulates adipogenic differentiation of human mesenchymal stem cells by targeting desmoplakin and inhibiting the Wnt/β-catenin pathway.

Cell death discovery

Yang Y,Fan J,Xu H,Fan L,Deng L,Li J,Li D,Li H,Zhang F,Zhao RC

Sat May 15 00:00:00 UTC 2021

ETV4 is a theranostic target in clear cell renal cell carcinoma that promotes metastasis by activating the pro-metastatic gene FOSL1 in a PI3K-AKT dependent manner.

Cancer letters

Xu L,Hu H,Zheng LS,Wang MY,Mei Y,Peng LX,Qiang YY,Li CZ,Meng DF,Wang MD,Liu ZJ,Li XJ,Huang BJ,Qian CN

Fri Jul 10 00:00:00 UTC 2020

Wilms' tumour 1-associating protein inhibits endothelial cell angiogenesis by m6A-dependent epigenetic silencing of desmoplakin in brain arteriovenous malformation.

Journal of cellular and molecular medicine

Wang LJ,Xue Y,Li H,Huo R,Yan Z,Wang J,Xu H,Wang J,Cao Y,Zhao JZ

Fri May 01 00:00:00 UTC 2020

Cdc42 Deficiency Leads To Epidermal Barrier Dysfunction by Regulating Intercellular Junctions and Keratinization of Epidermal Cells during Mouse Skin Development.

Theranostics

Zhang M,Wang X,Guo F,Jia Q,Liu N,Chen Y,Yan Y,Huang M,Tang H,Deng Y,Huang S,Zhou Z,Zhang L,Zhang L

Thu Aug 20 00:00:00 UTC 2020

Cdc42 Deficiency Leads To Epidermal Barrier Dysfunction by Regulating Intercellular Junctions and Keratinization of Epidermal Cells during Mouse Skin Development.

Theranostics

Zhang M,Wang X,Guo F,Jia Q,Liu N,Chen Y,Yan Y,Huang M,Tang H,Deng Y,Huang S,Zhou Z,Zhang L,Zhang L

Thu Aug 20 00:00:00 UTC 2020

GTSE1 is involved in breast cancer progression in p53 mutation-dependent manner.

Journal of experimental & clinical cancer research : CR

Lin F,Xie YJ,Zhang XK,Huang TJ,Xu HF,Mei Y,Liang H,Hu H,Lin ST,Luo FF,Lang YH,Peng LX,Qian CN,Huang BJ

Mon Apr 08 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: 250/210 kDa paraneoplastic pemphigus antigen; Desmoplakin; desmoplakin I/II; Desmoplakin II; Desmoplakin-I; desmosomal cytoskeletal connector molecule; DP

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Gene Aliases: 2300002E22Rik; 5730453H04Rik; AA407887; AA407888; AW109828; DCWHKTA; DP; DSP; rul

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UniProt ID: (Human) P15924, (Mouse) E9Q557

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Entrez Gene ID: (Human) 1832, (Mouse) 109620

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protein kinase C binding structural molecule activity structural constituent of cytoskeleton protein binding protein binding, bridging poly(A) RNA binding cell adhesion molecule binding cell adhesive protein binding involved in bundle of His cell-Purkinje myocyte communication scaffold protein binding

desmosome organization ventricular compact myocardium morphogenesis epidermis development peptide cross-linking keratinocyte differentiation adherens junction organization wound healing intermediate filament organization protein localization to adherens junction bundle of His cell-Purkinje myocyte adhesion involved in cell communication regulation of heart rate by cardiac conduction regulation of ventricular cardiac muscle cell action potential single organismal cell-cell adhesion skin development intermediate filament cytoskeleton organization

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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