Invitrogen

Digoxigenin Recombinant Rabbit Monoclonal Antibody (9H27L19)

1 Published Figure

14 References

View all (7) Digoxigenin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (1)

Digoxigenin Antibody in Western Blot (WB)

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Digoxigenin Antibody (700772) in WB

Western blot analysis of recombinant Digoxigenin conjugated to BSA (1 ng/lane) using a Digoxigenin recombinant rabbit monoclonal antibody (Product # 700772) at a dilution of 0.1 µg/mL. NBT/BCIP was used as the substrate (Product # WB7105).

Digoxigenin Antibody in Immunocytochemistry (ICC/IF)

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Product Details

700772

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.5 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

ELISA (ELISA)

View 2 publications 2 publications

In Situ Hybridization (ISH) (ISH)

View 2 publications 2 publications

Miscellaneous PubMed (MISC)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Chemical

Published species

Chemical, Fungi, Mouse, Rat, Zebrafish

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

9H27L19

Immunogen

proprietary

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

AB_2532342

Product Specific Information

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Digoxin, also known as digitalis, is a purified cardiac glycoside extracted from the foxglove plant, Digitalis lanata. Its corresponding aglycone is digoxigenin, and its acetyl derivative is acetyldigoxin. Digoxin is widely used in the treatment of various heart conditions, namely atrial fibrillation, atrial flutter and sometimes heart failure that cannot be controlled by other medication. Digoxin preparations are commonly marketed under the trade names Lanoxin, Digitek, and Lanoxicaps. Digoxin is also used as an affinity system for nonradioactive labeling and detection of proteins and nucleic acids.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Consequences of a telomerase-related fitness defect and chromosome substitution technology in yeast synIX strains.

Cell genomics

McCulloch LH,Sambasivam V,Hughes AL,Annaluru N,Ramalingam S,Fanfani V,Lobzaev E,Mitchell LA,Cai J,Jiang H,LaCava J,Taylor MS,Bishai WR,Stracquadanio G,Steinmetz LM,Bader JS,Zhang W,Boeke JD,Chandrasegaran S

700772 was used in Western Blot to identify and resolved a bug affecting expression of EST3, a crucial gene for telomerase function, producing a synIX strain with near wild-type fitness.

Wed Nov 08 00:00:00 UTC 2023

Origin of thyrotropin-releasing hormone neurons that innervate the tuberomammillary nuclei.

Brain structure & function

Sánchez-Jaramillo E,Wittmann G,Menyhért J,Singru P,Gómez-González GB,Sánchez-Islas E,Yáñez-Recendis N,Pimentel-Cabrera JA,León-Olea M,Gereben B,Fekete C,Charli JL,Lechan RM

700772 was used in Immunohistochemistry to conclude that, in rodents, the TRH innervation of TMN originates in part from TRH neurons in the TuLH, and that this TRH population may contribute to regulate energy homeostasis through histamine Trhr-positive neurons of the TMN.

Thu Sep 01 00:00:00 UTC 2022

Origin of thyrotropin-releasing hormone neurons that innervate the tuberomammillary nuclei.

Brain structure & function

Sánchez-Jaramillo E,Wittmann G,Menyhért J,Singru P,Gómez-González GB,Sánchez-Islas E,Yáñez-Recendis N,Pimentel-Cabrera JA,León-Olea M,Gereben B,Fekete C,Charli JL,Lechan RM

Thu Sep 01 00:00:00 UTC 2022

16pdel lipid changes in iPSC-derived neurons and function of FAM57B in lipid metabolism and synaptogenesis.

iScience

Tomasello DL,Kim JL,Khodour Y,McCammon JM,Mitalipova M,Jaenisch R,Futerman AH,Sive H

700772 was used in Immunohistochemistry to examine the function of the FAM57B gene to further assess the predicted connection with 16p11.2 deletion syndrome.

Fri Jan 21 00:00:00 UTC 2022

Identification of a genetic variant underlying familial cases of recurrent benign paroxysmal positional vertigo.

PloS one

Xu Y,Zhang Y,Lopez IA,Hilbers J,Griswold AJ,Ishiyama A,Blanton S,Liu XZ,Lundberg YW

700772 was used in Immunohistochemistry to show that a variant in the PCDHGA10 gene may be involved in causing or aggravating some familial cases of recurrent idiopathic BPPV.

Tue Oct 19 00:00:00 UTC 2021

Epigenetic cell fate in Candida albicans is controlled by transcription factor condensates acting at super-enhancer-like elements.

Nature microbiology

Frazer C,Staples MI,Kim Y,Hirakawa M,Dowell MA,Johnson NV,Hernday AD,Ryan VH,Fawzi NL,Finkelstein IJ,Bennett RJ

700772 was used in Flow Cytometry to reveal that PrLDs support the assembly of TF complexes that control fungal cell identity and highlight parallels with the 'super-enhancers' that regulate mammalian cell fate.

Sun Nov 01 00:00:00 UTC 2020

Multi-enhancer transcriptional hubs confer phenotypic robustness.

eLife

Tsai A,Alves MR,Crocker J

700772 was used in Immunohistochemistry to demonstrate a mechanism for phenotypical robustness by use of multiple enhancers which can reinforce a local transcriptional hub to buffer against environmental stresses and genetic pertubations.

Thu Jul 11 00:00:00 UTC 2019

RNA from a simple-tandem repeat is required for sperm maturation and male fertility in Drosophila melanogaster.

eLife

Mills WK,Lee YCG,Kochendoerfer AM,Dunleavy EM,Karpen GH

700772 was used in Immunocytochemistry to find AAGAG transcripts are necessary for male fertility, and that AAGAG RNA depletion results in defective histone-protamine exchange, sperm maturation and chromatin organization.

Tue Nov 05 00:00:00 UTC 2019

Selective Restoration of Pomc Expression in Glutamatergic POMC Neurons: Evidence for a Dynamic Hypothalamic Neurotransmitter Network.

eNeuro

Jones GL,Wittmann G,Yokosawa EB,Yu H,Mercer AJ,Lechan RM,Low MJ

700772 was used in In Situ Hybridization to study the role of glutamatergic POMC neurons in maintaining energy homeostasis.

Thu Mar 05 00:00:00 UTC 2020

Cadherin complexes recruit mRNAs and RISC to regulate epithelial cell signaling.

The Journal of cell biology

Kourtidis A,Necela B,Lin WH,Lu R,Feathers RW,Asmann YW,Thompson EA,Anastasiadis PZ

700772 was used in an In situ hybridization assay to reveal a mechanism that directly links junction integrity to the silencing of a set of mRNAs that critically affect epithelial homeostasis.

Mon Oct 02 00:00:00 UTC 2017

Dynamic DNA binding licenses a repair factor to bypass roadblocks in search of DNA lesions.

Nature communications

Brown MW,Kim Y,Williams GM,Huck JD,Surtees JA,Finkelstein IJ

Published figure using Digoxigenin monoclonal antibody (Product # 700772) in Immunofluorescence

Wed Feb 03 00:00:00 UTC 2016

High-Throughput Universal DNA Curtain Arrays for Single-Molecule Fluorescence Imaging.

Langmuir : the ACS journal of surfaces and colloids

Gallardo IF,Pasupathy P,Brown M,Manhart CM,Neikirk DP,Alani E,Finkelstein IJ

700772 was used in ELISA to report a high-throughput method for DNA curtain studies

Tue Sep 22 00:00:00 UTC 2015

Isoform-specific expression of the neuropeptide orcokinin in Drosophila melanogaster.

Peptides

Chen J,Choi MS,Mizoguchi A,Veenstra JA,Kang K,Kim YJ,Kwon JY

700772 was used in immunocytochemistry to examine the distribution of orcokinin isoforms in Drosophilia

Mon Jun 01 00:00:00 UTC 2015

Targeting epithelial-to-mesenchymal transition with Met inhibitors reverts chemoresistance in small cell lung cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research

Cañadas I,Rojo F,Taus Á,Arpí O,Arumí-Uría M,Pijuan L,Menéndez S,Zazo S,Dómine M,Salido M,Mojal S,García de Herreros A,Rovira A,Albanell J,Arriola E

700772 was used in in situ hybridization to study the ability of Met inhibitors to reverse chemoresistance in small cell lung cancer and the role of epithelial-to-mesenchymal transition

Sat Feb 15 00:00:00 UTC 2014

Application of DNA Aptamers and Quantum Dots to Lateral Flow Test Strips for Detection of Foodborne Pathogens with Improved Sensitivity versus Colloidal Gold.

Pathogens (Basel, Switzerland)

Bruno JG

700772 was used in ELISA to report a proof-of-principle assay for the development of a highly sensitive aptamer-Qdot LF strip assay for rapid foodborne pathogen detection.

Thu Apr 10 00:00:00 UTC 2014

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Digoxigenin Recombinant Rabbit Monoclonal Antibody (9H27L19)

Digoxigenin Antibody (700772) in WB

Product Details

700772

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

In Situ Hybridization (ISH) (ISH)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Performance Guarantee

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