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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Dinitrophenyl-KLH Antibodies

          Invitrogen

          Dinitrophenyl-KLH Polyclonal Antibody, Alexa Fluor™ 488

          4 Published Figures
          28 References

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          Datasheet
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          Cite Dinitrophenyl-KLH Polyclonal Antibody, Alexa Fluor™ 488

          • Published Figures (4)
          Dinitrophenyl-KLH Antibody in Immunocytochemistry (ICC/IF)
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          Dinitrophenyl-KLH Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 4

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          Dinitrophenyl-KLH Antibody (A-11097) in ICC/IF

          American journal of physiology. Cell physiology 2011 - Published figure using Dinitrophenyl-KLH polyclonal antibody (Product # A-11097) in Immunocytochemistry {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Dinitrophenyl-KLH Antibody in Immunocytochemistry (ICC/IF)
          Dinitrophenyl-KLH Antibody in Immunohistochemistry (IHC)
          Dinitrophenyl-KLH Antibody in Flow Cytometry (Flow)
          Dinitrophenyl-KLH Antibody in Flow Cytometry (Flow)

          Product Details

          A-11097

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          Assay-dependent
          View 3 publications 3 publications

          Immunohistochemistry (IHC)

          Assay-dependent
          View 13 publications 13 publications

          Immunocytochemistry (ICC/IF)

          Assay-dependent
          View 6 publications 6 publications

          Flow Cytometry (Flow)

          -
          View 5 publications 5 publications

          Miscellaneous PubMed (MISC)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Chemical

          Published species

          Chemical

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Dinitrophenyl-KLH

          Conjugate

          Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

          Excitation/Emission Max

          499/520 nm View spectra spectra

          Form

          Liquid

          Concentration

          2 mg/mL

          Purification

          purified

          Storage buffer

          PBS, pH 7.5

          Contains

          5mM sodium azide

          Storage conditions

          4°C, store in dark

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2534131

          Product Specific Information

          The anti-DNP-KLH antibody is supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, containing 5 mM sodium azide. The degree of labeling is indicated on the product label. This antibody was raised in rabbit against DNP conjugated to keyhole limpet hemocyanin. The IgG fraction, which contains both anti-DNP and anti-KLH IgGs, was isolated from total serum and determined to be >95% pure by SDS-polyacrylamide gel electrophoresis. It is a good practice to centrifuge protein conjugate solutions briefly in a microcentrifuge before use; only the supernatant should then be added to the experiment. This step will eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. Peak absorption and fluorescence emission wavelengths for Alexa Fluor™ 488 anti-DNPKLH conjugate is 494 nm and 520 nm, respectively

          Because of their high affinity for the dinitrophenyl hapten1,2 (DNP), Invitrogen's antibodies against the dinitrophenyl- keyhole limpet hemocyanin conjugate (anti-DNP-KLH) are sensitive probes for detecting DNP-labeled molecules. The fluorescent and biotinylated anti-DNP-KLH conjugates can serve as second-step reagents for signal amplification in protein and nucleic acid detection systems, including in situ hybridization studies employing DNP-modified DNA probes. Because anti-DNP antibody binds well to DAMP, an acidotropic reagent that can penetrate cell membranes, anti-DNP-KLH antibodies can also be used with DAMP for immunochemical localization of intracellular compartments with low pH. In addition, anti-DNP-KLH antibodies may recognize compounds with a trinitrophenyl group, allowing the isolation of cell-surface molecules of human melanoma cells that have been labeled with trinitrobenzene sulfonic acid (TNBS).

          Target Information

          DNP-KLH antibodies are used for detecting DNP-labeled molecules. Fluorescent and biotinylated anti-DNP-KLH conjugates can serve as second-step reagents for signal amplification in protein and nucleic acid detection systems, including in situ hybridization studies employing DNP-modified DNA probes. Because anti-DNP antibody binds well to DAMP (D-1552), an acidotropic reagent that can penetrate cell membranes, anti-DNP-KLH antibodies can also be used with DAMP for immunochemical localization of intracellular compartments with low pH. DNP-KLH antibodies may recognize compounds with a trinitrophenyl group, allowing the isolation of cell-surface molecules of human melanoma cells that have been labeled with trinitrobenzene sulfonic acid (TNBS).

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: C6H3N2O4; DNP-KLH

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          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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