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Product Details
PA1-7212
Product Specifications
Species Reactivity
Bacteria
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Purified diphtheria toxoid.
Conjugate
Form
Liquid
Concentration
4-5 mg/mL
Purification
IgG fraction
Storage buffer
PBS, pH 7.2
Contains
0.1% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_558645
Product Specific Information
PA1-7212 detects diphtheria toxin.
PA1-7212 has been successfully used in ELISA procedures.
The PA1-7212 immunogen is purified diphtheria toxoid.
For longer term storage, aliquot and freeze at -20ºC.
Target Information
Diphtheria toxin is a 58 kDa protein secreted by lysogenic strains of Corynebacterium diphtheriae. The toxin causes the disease diphtheria in humans by gaining entry into the cell cytoplasm and inhibiting protein synthesis. The mechanism of inhibition involves transfer of the ADP-ribose group of NAD to elongation factor-2 (EF-2), rendering EF-2 inactive. The catalysed reaction is as follows: NAD + + peptide diphthamide = nicotinamide + peptide N-(ADP-D-ribosyl)diphthamide The crystal structure of the diphtheria toxin homodimer has been determined to 2.5A resolution. The structure reveals a Y-shaped molecule of 3 domains, a catalytic domain (fragment A), whose fold is of the alpha + beta type; a transmembrane (TM) domain, which consists of 9 alpha-helices, 2 pairs of which may participate in pH-triggered membrane insertion and translocation; and a receptor-binding domain, which forms a flattened beta-barrel with a jelly-roll-like topology. The TM- and receptor binding-domains together constitute fragment B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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