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Invitrogen
Donkey anti-Mouse IgG (H+L) ReadyProbes™ Secondary Antibody, Alexa Fluor™ 488
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FIGURE: 1 / 16
Mouse IgG (H+L) Secondary Antibody (R37114) in IHC (F)
Immunofluorescent analysis of beta-3 Tubulin (green) and PAX6 (red) in human iPSC-derived forebrain organoids derived at Day 40. The organoids were fixed with 4% PFA for 1 hour at room temperature, followed by incubation with 30% sucrose solution overnight at 4°C. The organoids were then embedded in OCT and cryosectioned at 5 µm, permeabilized with 0.2% Triton X-100 for 20 min, and blocked with 10% donkey s... View More
Product Details
R37114
Applications
Tested Dilution
Western Blot (WB)
1:2,500-1:5,000
Immunohistochemistry (Frozen) (IHC (F))
Assay-Dependent
Immunocytochemistry (ICC/IF)
1:2,000
Flow Cytometry (Flow)
Assay-Dependent
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Donkey
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Gamma Immunoglobin
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Purification
Affinity chromatography
Storage buffer
PBS with 0.1% BSA
Contains
<0.1% sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2556542
Target
IgG
Antibody Form
Whole Antibody
Product Specific Information
No pipetting or dilutions required. Just tip and drip two drops per mL buffer to stain your cells.
This antibody shows minimum cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat, and sheep serum proteins. Like other ReadyProbes reagents, Alexa Fluor 488 Donkey anti-Mouse IgG Antibody comes in a convenient dropper bottle.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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