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Invitrogen
Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, TRITC
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FIGURE: 1 / 1
Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A16040) in ICC/IF
Immunofluorescence analysis of Donkey anti-Rabbit IgG (H+L) Secondary Antibody, TRITC was performed using HeLa cells stained with alpha Tubulin Rabbit Polyclonal Primary Antibody (Product # PA5-16891). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2 µg/mL rabbit primary antibody for 3 hours ... View More
Product Details
A16040
Applications
Tested Dilution
Immunocytochemistry (ICC/IF)
4 µg/mL
Product Specifications
Species Reactivity
Rabbit
Host/Isotype
Donkey
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Gamma Immunoglobins Heavy and Light chains
Conjugate
Excitation/Emission Max
552/578 nm
View spectra
Form
Lyophilized
Concentration
2.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2534714
Target
IgG
Cross Adsorption
Against bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat and sheep IgG
Antibody Form
Whole Antibody
Product Specific Information
Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily. 1 year from date of receipt. Prepare working dilution prior to use and then discard. Based on Immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins, IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat or sheep.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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